Restriction map for pF1KE0913
 (Linear) (Six-base) MAPSORT of: KIEE0913.seq  Check: 2026  from: 1  to: 762



KIEE0913.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AfeI AGC'GCT

Cuts at:      0      57     301     762 
   Size:         57     244     461

  Fragments arranged by size: 

                 461     244      57
 
AflIII A'CryG_T

Cuts at:      0      87     762 
   Size:         87     675
 
AhdI GACnn_n'nnGTC

Cuts at:      0     677     762 
   Size:        677      85
 
AleI CACnn'nnGTG

Cuts at:      0     466     762 
   Size:        466     296
 
ApoI r'AATT_y

Cuts at:      0     214     762 
   Size:        214     548
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     311     344     762 
   Size:        311      33     418

  Fragments arranged by size: 

                 418     311      33
 
BanII G_rGCy'C

Cuts at:      0     380     762 
   Size:        380     382
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     474     503     762 
   Size:        474      29     259

  Fragments arranged by size: 

                 474     259      29
 
BclI T'GATC_A

Cuts at:      0      92     762 
   Size:         92     670
 
Bme1580I G_kGCm'C

Cuts at:      0     663     762 
   Size:        663      99
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     585     615     762 
   Size:        585      30     147

  Fragments arranged by size: 

                 585     147      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     762 
   Size:        317     445
 
Bpu10I CC'TnA_GC

Cuts at:      0      59     762 
   Size:         59     703
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     583     762 
   Size:        227     356     179

  Fragments arranged by size: 

                 356     227     179
 
BsaAI yAC'GTr

Cuts at:      0     434     762 
   Size:        434     328
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       5      35     762 
   Size:          5      30     727

  Fragments arranged by size: 

                 727      30       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     350     575     762 
   Size:        350     225     187

  Fragments arranged by size: 

                 350     225     187
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     187     762 
   Size:        187     575
 
Bsp1286I G_dGCh'C

Cuts at:      0     350     380     575     663     762 
   Size:        350      30     195      88      99

  Fragments arranged by size: 

                 350     195      99      88      30
 
BspHI T'CATG_A

Cuts at:      0     290     762 
   Size:        290     472
 
BsrDI GCAATG_nn'

Cuts at:      0     741     762 
   Size:        741      21
 
BstBI TT'CG_AA

Cuts at:      0     441     762 
   Size:        441     321
 
EaeI y'GGCC_r

Cuts at:      0       2     254     762 
   Size:          2     252     508

  Fragments arranged by size: 

                 508     252       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     599     762 
   Size:        599     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     762 
   Size:        317     445
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      23     762 
   Size:         23     739
 
EcoO109I rG'GnC_Cy

Cuts at:      0     754     762 
   Size:        754       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     520     762 
   Size:        520     242
 
FspI TGC'GCA

Cuts at:      0     727     762 
   Size:        727      35
 
HaeII r_GCGC'y

Cuts at:      0      59     303     762 
   Size:         59     244     459

  Fragments arranged by size: 

                 459     244      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      20      42      52      74     663     695     762 
   Size:         20      22      10      22     589      32      67

  Fragments arranged by size: 

                 589      67      32      22      22      20      10
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     762 
   Size:        329     433
 
MscI TGG'CCA

Cuts at:      0       4     256     762 
   Size:          4     252     506

  Fragments arranged by size: 

                 506     252       4
 
MslI CAynn'nnrTG

Cuts at:      0      43     265     466     762 
   Size:         43     222     201     296

  Fragments arranged by size: 

                 296     222     201      43
 
NspI r_CATG'y

Cuts at:      0      91     762 
   Size:         91     671
 
PasI CC'CwG_GG

Cuts at:      0     491     663     762 
   Size:        491     172      99

  Fragments arranged by size: 

                 491     172      99
 
PciI A'CATG_T

Cuts at:      0      87     762 
   Size:         87     675
 
PmlI CAC'GTG

Cuts at:      0     434     762 
   Size:        434     328
 
PstI C_TGCA'G

Cuts at:      0     565     762 
   Size:        565     197
 
SfcI C'TryA_G

Cuts at:      0     561     641     762 
   Size:        561      80     121

  Fragments arranged by size: 

                 561     121      80
 
SmlI C'TyrA_G

Cuts at:      0     242     598     762 
   Size:        242     356     164

  Fragments arranged by size: 

                 356     242     164
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       5      37     762 
   Size:          5      32     725

  Fragments arranged by size: 

                 725      32       5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     264     762 
   Size:        264     498
 

 Enzymes that do cut:

     AfeI   AflIII     AhdI     AleI     ApoI     BaeI    BanII     BbsI
     BclI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrDI    BstBI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I     MmeI
     MscI     MslI     NspI     PasI     PciI     PmlI     PstI     SfcI
     SmlI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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