Restriction map for pF1KE0911
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BsrI        Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI          199  GGCGTC               G[GA]^CG[CT]C
ApaI          161  GGGCCC               GGGCC^C
Asp700I       513  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          121  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         488  GGATCC               G^GATCC
BbsI          712  GAAGAC               GAAGAC(2/6)
BfaI          686  CTAG                 C^TAG
BglI          320  GCCGGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         712  GAAGAC               GAAGAC(2/6)
BsaHI         199  GGCGTC               G[GA]^CG[CT]C
BseRI         253  GAGGAG               GAGGAG(10/8)
BsmAI         202  GTCTC                GTCTC(1/5)
BsmBI         201  CGTCTC               CGTCTC(1/5)
BspHI          17  TCATGA               T^CATGA
BsrDI         358  GCAATG               GCAATG(2/0)
BssHII        192  GCGCGC               G^CGCGC
BssSI          70  CACGAG               CACGAG(-5/-1)
BstAPI        434  GCATCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        468  GGATG                GGATG(2/0)
BstYI         488  GGATCC               [GA]^GATC[CT]
DdeI          353  CTAAG                C^T[ACGT]AG
DrdI          295  GACAGGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          174  CGGCCA               [CT]^GGCC[GA]
EcoNI         102  CCTGGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          468  GGATG                GGATG(9/13)
Hin1I         199  GGCGTC               G[GA]^CG[CT]C
MaeI          686  CTAG                 C^TAG
MflI          488  GGATCC               [GA]^GATC[CT]
MseI          682  TTAA                 T^TAA
PflFI         121  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          551  GACTC                GAGTC(4/5)
RcaI           17  TCATGA               T^CATGA
SgrAI         195  CGCCGGCG             C[GA]^CCGG[CT]G
TaqI          293  TCGA                 T^CGA
TfiI          697  GAATC                G^A[AT]TC
Tru9I         682  TTAA                 T^TAA
Tth111I       121  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       293  TCGA                 T^CGA
XhoII         488  GGATCC               [GA]^GATC[CT]
XmnI          513  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          670  AAATTC               [GA]^AATT[CT]
              678  GAATTT              
AfaI           51  GTAC                 GT^AC
              633  GTAC                
ApoI          670  AAATTC               [GA]^AATT[CT]
              678  GAATTT              
AvaII          27  GGACC                G^G[AT]CC
              303  GGTCC               
BanII         161  GGGCCC               G[GA]GC[CT]^C
              535  GAGCCC              
BbrPI         234  CACGTG               CAC^GTG
              403  CACGTG              
BcnI          158  CCGGG                CC^[GC]GG
              376  CCGGG               
BsaAI         234  CACGTG               [CT]AC^GT[GA]
              403  CACGTG              
BspMI         215  ACCTGC               ACCTGC(4/8)
              439  ACCTGC              
BsrFI         196  GCCGGC               [GA]^CCGG[CT]
              320  GCCGGC              
Cfr10I        196  GCCGGC               [GA]^CCGG[CT]
              320  GCCGGC              
DraII         160  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              161  GGGCCCT             
DraIII         70  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              586  CACGAAGTG           
EcoO109I      160  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              161  GGGCCCT             
HinfI         551  GACTC                G^A[ACGT]TC
              697  GAATC               
HphI          421  GGTGA                GGTGA(8/7)
              437  TCACC               
MspA1I        145  CAGCTG               C[AC]G^C[GT]G
              178  CAGCTG              
NaeI          196  GCCGGC               GCC^GGC
              320  GCCGGC              
NciI          158  CCGGG                CC^[GC]GG
              376  CCGGG               
NgoMIV        196  GCCGGC               G^CCGGC
              320  GCCGGC              
NlaIII         18  CATG                 CATG^
              481  CATG                
PmaCI         234  CACGTG               CAC^GTG
              403  CACGTG              
PmlI          234  CACGTG               CAC^GTG
              403  CACGTG              
PvuII         145  CAGCTG               CAG^CTG
              178  CAGCTG              
RsaI           51  GTAC                 GT^AC
              633  GTAC                
TspRI         151  CACTG                CA[GC]TG[ACGT][ACGT]^
              708  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbvI        
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DpnII       
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       ItaI        
MaeII       MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp509I     
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