Restriction map for pF1KE0907
##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BseAI       
BsgI        BsiHKAI     BsiWI       BsiYI       BslI        
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeIII      HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TseI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          544  GTATAC               GT^[AC][GT]AC
AfaI          225  GTAC                 GT^AC
BanII         218  GGGCTC               G[GA]GC[CT]^C
BbsI          208  GAAGAC               GAAGAC(2/6)
BpuAI         208  GAAGAC               GAAGAC(2/6)
BsaWI          15  ACCGGA               [AT]^CCGG[AT]
BseRI          55  GAGGAG               GAGGAG(10/8)
BsiEI         378  CGATCG               CG[GA][CT]^CG
BsmBI         363  GAGACG               CGTCTC(1/5)
BsmFI         594  GTCCC                GGGAC(10/14)
Bsp1407I      224  TGTACA               T^GTACA
BspMI         176  ACCTGC               ACCTGC(4/8)
BsrBI         618  GAGCGG               CCGCTC(-3/-3)
BsrGI         224  TGTACA               T^GTACA
BsrI          237  CCAGT                ACTGG(1/-1)
BssKI         259  CCTGG                ^CC[ACGT]GG
Bst1107I      544  GTATAC               GTA^TAC
BstNI         259  CCTGG                CC^[AT]GG
BstZ17I       544  GTATAC               GTA^TAC
Cac8I         168  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          452  GCGC                 GCG^C
DdeI          614  CTAAG                C^T[ACGT]AG
DraI          142  TTTAAA               TTT^AAA
EarI           52  GAAGAG               CTCTTC(1/4)
EcoRII        259  CCTGG                ^CC[AT]GG
HaeII         451  GGCGCT               [GA]GCGC^[CT]
HapII          16  CCGG                 C^CGG
HhaI          452  GCGC                 GCG^C
HinP1I        452  GCGC                 G^CGC
HpaII          16  CCGG                 C^CGG
HphI          478  GGTGA                GGTGA(8/7)
Ksp632I        52  GAAGAG               CTCTTC(1/4)
MslI          398  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           16  CCGG                 C^CGG
MvaI          259  CCTGG                CC^[AT]GG
MwoI          601  GCAGACACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         622  GGCTCC               GG[ACGT]^[ACGT]CC
PvuI          378  CGATCG               CGAT^CG
RsaI          225  GTAC                 GT^AC
ScrFI         259  CCTGG                CC^[ACGT]GG
SspBI         224  TGTACA               T^GTACA
TspRI         435  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI          180  GCGG                 CCGC(-3/-1)
              620  GCGG                
BfaI          245  CTAG                 C^TAG
              391  CTAG                
BmyI          128  GGGCAC               G[AGT]GC[ACT]^C
              218  GGGCTC              
BsmAI         363  GAGAC                GTCTC(1/5)
              394  GAGAC               
Bsp1286I      128  GGGCAC               G[AGT]GC[ACT]^C
              218  GGGCTC              
BstF5I        109  CATCC                GGATG(2/0)
              285  GGATG               
DpnII         186  GATC                 ^GATC
              379  GATC                
Fnu4HI        180  GCGGC                GC^[ACGT]GC
              620  GCGGC               
FokI          109  CATCC                GGATG(9/13)
              285  GGATG               
HinfI          67  GAATC                G^A[ACGT]TC
              418  GAATC               
ItaI          180  GCGGC                GC^[ACGT]GC
              620  GCGGC               
MaeI          245  CTAG                 C^TAG
              391  CTAG                
MboI          186  GATC                 ^GATC
              379  GATC                
NdeII         186  GATC                 ^GATC
              379  GATC                
Sau3AI        186  GATC                 ^GATC
              379  GATC                
TaqI           11  TCGA                 T^CGA
              377  TCGA                
TfiI           67  GAATC                G^A[AT]TC
              418  GAATC               
Tsp45I        266  GTGAC                ^GT[GC]AC
              371  GTGAC               
Tsp509I       250  AATT                 ^AATT
              342  AATT                
TthHB8I        11  TCGA                 T^CGA
              377  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AluI        MaeIII      MboII       MnlI        MseI        
SfcI        Tru9I       
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