Restriction map for
pF1KE0738
(Circular) (Six-base) MAPSORT of: pF1KE0738.seq Check: 8222 from: 1 to: 4385
pF1KE0738 4385 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 438 438
Size: 4385
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 984 1337 2210 2642 3389 3657 582
Size: 402 353 873 432 747 268 1310
Fragments arranged by size:
1310 873 747 432 402 353 268
AfeI AGC'GCT
Cuts at: 304 304
Size: 4385
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AleI CACnn'nnGTG
Cuts at: 1057 1057
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 372 384 1103 1690 3257 372
Size: 12 719 587 1567 1500
Fragments arranged by size:
1567 1500 719 587 12
ApaI G_GGCC'C
Cuts at: 415 415
Size: 4385
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 1389 3738 3994 1389
Size: 2349 256 1780
Fragments arranged by size:
2349 1780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 146 884 899 1224 1405 146
Size: 738 15 325 181 3126
Fragments arranged by size:
3126 738 325 181 15
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 151 1357 1401 1815 2063 2098 151
Size: 1206 44 414 248 35 2438
Fragments arranged by size:
2438 1206 414 248 44 35
BanII G_rGCy'C
Cuts at: 415 649 993 1399 2429 415
Size: 234 344 406 1030 2371
Fragments arranged by size:
2371 1030 406 344 234
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbsI GAAGACnn'nnnn_
Cuts at: 606 606
Size: 4385
BbvCI CC'TCA_GC
Cuts at: 1273 1279 1285 1273
Size: 6 6 4373
Fragments arranged by size:
4373 6 6
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 293 293
Size: 4385
BglII A'GATC_T
Cuts at: 782 1900 3937 782
Size: 1118 2037 1230
Fragments arranged by size:
2037 1230 1118
BlpI GC'TnA_GC
Cuts at: 454 1497 454
Size: 1043 3342
Bme1580I G_kGCm'C
Cuts at: 154 415 2010 2103 3159 154
Size: 261 1595 93 1056 1380
Fragments arranged by size:
1595 1380 1056 261 93
BmrI ACTGGGnnnn_n'
Cuts at: 1597 1678 2013 4157 1597
Size: 81 335 2144 1825
Fragments arranged by size:
2144 1825 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 858 1239 520
Size: 338 381 3666
Fragments arranged by size:
3666 381 338
Bpu10I CC'TnA_GC
Cuts at: 648 870 1273 1279 1285 648
Size: 222 403 6 6 3748
Fragments arranged by size:
3748 403 222 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 1561 2440 2932 3230 593
Size: 968 879 492 298 1748
Fragments arranged by size:
1748 968 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1360 4089 1360
Size: 2729 1656
BsaAI yAC'GTr
Cuts at: 1738 1738
Size: 4385
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 842 214
Size: 628 3757
BseYI C'CCAG_C
Cuts at: 186 291 665 986 1184 1810 2568 3145 3641
Size: 105 374 321 198 626 758 577 496
Cuts at: 3641 186
Size: 930
Fragments arranged by size:
930 758 626 577 496 374 321 198
105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 447 313
Size: 134 4251
BsiEI CG_ry'CG
Cuts at: 104 254 1927 1973 3181 104
Size: 150 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 150 46
BsiHKAI G_wGCw'C
Cuts at: 455 1399 2177 2367 3159 455
Size: 944 778 190 792 1681
Fragments arranged by size:
1681 944 792 778 190
BsmI GAATG_Cn'
Cuts at: 1038 1038
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 154 415 455 649 993 1399 2010 2103 2177
Size: 261 40 194 344 406 611 93 74
Cuts at: 2177 2367 2429 3159 154
Size: 190 62 730 1380
Fragments arranged by size:
1380 730 611 406 344 261 194 190
93 74 62 40
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 438 452 569 1435 1649 1951 2332 438
Size: 14 117 866 214 302 381 2491
Fragments arranged by size:
2491 866 381 302 214 117 14
BsrBI CCG'CTC
Cuts at: 50 1253 2677 2731 4215 4380 50
Size: 1203 1424 54 1484 165 55
Fragments arranged by size:
1484 1424 1203 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2297 2297
Size: 4385
BsrFI r'CCGG_y
Cuts at: 738 1199 2383 2564 2773 738
Size: 461 1184 181 209 2350
Fragments arranged by size:
2350 1184 461 209 181
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 1762 1762
Size: 4385
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstEII G'GTnAC_C
Cuts at: 741 741
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 240 495 1059 240
Size: 255 564 3566
Fragments arranged by size:
3566 564 255
BstYI r'GATC_y
Cuts at: 1 782 935 1115 1410 1877 1900 2235 2481
Size: 781 153 180 295 467 23 335 246
Cuts at: 2481 3493 3579 3591 3937 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 781 467 449 346 335 295 246
180 153 86 23 12
BtgI C'CryG_G
Cuts at: 107 353 694 1147 107
Size: 246 341 453 3345
Fragments arranged by size:
3345 453 341 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 552 963 2335 2516 2679 552
Size: 411 1372 181 163 2258
Fragments arranged by size:
2258 1372 411 181 163
BtsI GCAGTG_nn'
Cuts at: 1628 1628
Size: 4385
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 206 230 350 485 587 1265 1970 2144 2535
Size: 24 120 135 102 678 705 174 391
Cuts at: 2535 2562 3907 206
Size: 27 1345 684
Fragments arranged by size:
1345 705 684 678 391 174 135 120
102 27 24
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 367 1313 2408 2618 367
Size: 946 1095 210 2134
Fragments arranged by size:
2134 1095 946 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 582 858 984 1239 1337 2210 2642 3389
Size: 62 276 126 255 98 873 432 747
Cuts at: 3389 3657 520
Size: 268 1248
Fragments arranged by size:
1248 873 747 432 276 268 255 126
98 62
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 4385
EcoO109I rG'GnC_Cy
Cuts at: 411 412 854 1180 1524 411
Size: 1 442 326 344 3272
Fragments arranged by size:
3272 442 344 326 1
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 972 2404 972
Size: 1432 2953
FspI TGC'GCA
Cuts at: 529 2166 529
Size: 1637 2748
HaeII r_GCGC'y
Cuts at: 306 361 1819 2067 3089 4068 306
Size: 55 1458 248 1022 979 623
Fragments arranged by size:
1458 1022 979 623 248 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 841 873 2227 2257 2259 2289 841
Size: 32 1354 30 2 30 2937
Fragments arranged by size:
2937 1354 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1235 3056 3240 1235
Size: 1821 184 2380
Fragments arranged by size:
2380 1821 184
MscI TGG'CCA
Cuts at: 208 232 487 2146 208
Size: 24 255 1659 2447
Fragments arranged by size:
2447 1659 255 24
MslI CAynn'nnrTG
Cuts at: 475 1057 1075 2374 2501 475
Size: 582 18 1299 127 2359
Fragments arranged by size:
2359 1299 582 127 18
MspA1I CmG'CkG
Cuts at: 291 381 1100 1149 1259 1363 1496 1810 2170
Size: 90 719 49 110 104 133 314 360
Cuts at: 2170 3183 3428 291
Size: 1013 245 1248
Fragments arranged by size:
1248 1013 719 360 314 245 133 110
104 90 49
NaeI GCC'GGC
Cuts at: 1201 2566 1201
Size: 1365 3020
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 107
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 1199 2564 1199
Size: 1365 3020
NspI r_CATG'y
Cuts at: 315 462 480 1438 1938 2845 315
Size: 147 18 958 500 907 1855
Fragments arranged by size:
1855 958 907 500 147 18
PasI CC'CwG_GG
Cuts at: 282 282
Size: 4385
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 1180 1180
Size: 4385
PshAI GACnn'nnGTC
Cuts at: 958 958
Size: 4385
PspOMI G'GGCC_C
Cuts at: 411 411
Size: 4385
PstI C_TGCA'G
Cuts at: 449 1432 2117 449
Size: 983 685 2717
Fragments arranged by size:
2717 983 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 291 381 1100 1259 1810 2170 291
Size: 90 719 159 551 360 2506
Fragments arranged by size:
2506 719 551 360 159 90
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SacII CC_GC'GG
Cuts at: 1150 1150
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SanDI GG'GwC_CC
Cuts at: 1180 1180
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 367 2408 2618 367
Size: 2041 210 2134
Fragments arranged by size:
2134 2041 210
SbfI CC_TGCA'GG
Cuts at: 449 1432 449
Size: 983 3402
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 445 762 1428 2113 3106 3297 33
Size: 412 317 666 685 993 191 1121
Fragments arranged by size:
1121 993 685 666 412 317 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 293 293
Size: 4385
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SgrAI Cr'CCGG_yG
Cuts at: 1199 1199
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 608 1224 1540 2455 2947 3209 608
Size: 616 316 915 492 262 1784
Fragments arranged by size:
1784 915 616 492 316 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 315 1438 1938 315
Size: 1123 500 2762
Fragments arranged by size:
2762 1123 500
StuI AGG'CCT
Cuts at: 814 1314 814
Size: 500 3885
StyI C'CwwG_G
Cuts at: 107 209 685 1327 1519 1873 107
Size: 102 476 642 192 354 2619
Fragments arranged by size:
2619 642 476 354 192 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1968 2101 2560 2769 1968
Size: 133 459 209 3584
Fragments arranged by size:
3584 459 209 133
TatI w'GTAC_w
Cuts at: 3900 3900
Size: 4385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 383 1880 383
Size: 1497 2888
XhoI C'TCGA_G
Cuts at: 1224 1224
Size: 4385
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PsiI PspXI PsrI
SexAI SnaBI SrfI SspI SwaI XmnI ZraI