Restriction map for
pF1KE0736
(Linear) (Six-base) MAPSORT of: KIEE0736.seq Check: 8341 from: 1 to: 1269
KIEE0736.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1149 1269
Size: 356 793 120
Fragments arranged by size:
793 356 120
AflIII A'CryG_T
Cuts at: 0 1232 1269
Size: 1232 37
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1211 1269
Size: 1211 58
ApaI G_GGCC'C
Cuts at: 0 546 1269
Size: 546 723
ApaLI G'TGCA_C
Cuts at: 0 1186 1269
Size: 1186 83
AvaI C'yCGr_G
Cuts at: 0 554 1269
Size: 554 715
BanII G_rGCy'C
Cuts at: 0 185 381 419 457 546 773 911 1269
Size: 185 196 38 38 89 227 138 358
Fragments arranged by size:
358 227 196 185 138 89 38 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 1269
Size: 323 946
BlpI GC'TnA_GC
Cuts at: 0 360 1269
Size: 360 909
Bme1580I G_kGCm'C
Cuts at: 0 546 1190 1269
Size: 546 644 79
Fragments arranged by size:
644 546 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 789 1269
Size: 789 480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 204 1269
Size: 204 1065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 799 1269
Size: 73 726 470
Fragments arranged by size:
726 470 73
Bpu10I CC'TnA_GC
Cuts at: 0 767 898 1269
Size: 767 131 371
Fragments arranged by size:
767 371 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1269
Size: 1066 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 1045 1269
Size: 77 968 224
Fragments arranged by size:
968 224 77
BsaHI Gr'CG_yC
Cuts at: 0 995 1269
Size: 995 274
BsaWI w'CCGG_w
Cuts at: 0 43 745 807 1259 1269
Size: 43 702 62 452 10
Fragments arranged by size:
702 452 62 43 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 135 165 1269
Size: 5 30 100 30 1104
Fragments arranged by size:
1104 100 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 526 610 853 1269
Size: 39 487 84 243 416
Fragments arranged by size:
487 416 243 84 39
BseYI C'CCAG_C
Cuts at: 0 122 772 910 1143 1269
Size: 122 650 138 233 126
Fragments arranged by size:
650 233 138 126 122
BsiHKAI G_wGCw'C
Cuts at: 0 1190 1269
Size: 1190 79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 155 1269
Size: 155 1114
Bsp1286I G_dGCh'C
Cuts at: 0 185 381 419 457 546 773 911 1190
Size: 185 196 38 38 89 227 138 279
Cuts at: 1190 1269
Size: 79
Fragments arranged by size:
279 227 196 185 138 89 79 38
38
BspEI T'CCGG_A
Cuts at: 0 1259 1269
Size: 1259 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1013 1269
Size: 1013 256
BsrBI CCG'CTC
Cuts at: 0 630 1269
Size: 630 639
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1035 1269
Size: 1035 234
BstYI r'GATC_y
Cuts at: 0 446 936 1269
Size: 446 490 333
Fragments arranged by size:
490 446 333
Bsu36I CC'TnA_GG
Cuts at: 0 593 1269
Size: 593 676
BtgI C'CryG_G
Cuts at: 0 206 1269
Size: 206 1063
BtsI GCAGTG_nn'
Cuts at: 0 165 943 1269
Size: 165 778 326
Fragments arranged by size:
778 326 165
DrdI GACnn_nn'nnGTC
Cuts at: 0 661 1269
Size: 661 608
EaeI y'GGCC_r
Cuts at: 0 440 831 1253 1269
Size: 440 391 422 16
Fragments arranged by size:
440 422 391 16
EarI CTCTTCn'nnn_
Cuts at: 0 808 1269
Size: 808 461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 108 261 1269
Size: 108 153 1008
Fragments arranged by size:
1008 153 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 356 799 1149 1269
Size: 73 283 443 350 120
Fragments arranged by size:
443 350 283 120 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 1269
Size: 571 698
EcoO109I rG'GnC_Cy
Cuts at: 0 16 390 542 543 587 1269
Size: 16 374 152 1 44 682
Fragments arranged by size:
682 374 152 44 16 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1083 1269
Size: 1083 186
FspI TGC'GCA
Cuts at: 0 265 1042 1269
Size: 265 777 227
Fragments arranged by size:
777 265 227
HaeII r_GCGC'y
Cuts at: 0 32 794 1269
Size: 32 762 475
Fragments arranged by size:
762 475 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 1269
Size: 456 32 781
Fragments arranged by size:
781 456 32
HincII GTy'rAC
Cuts at: 0 654 1269
Size: 654 615
MscI TGG'CCA
Cuts at: 0 1255 1269
Size: 1255 14
MslI CAynn'nnrTG
Cuts at: 0 1105 1136 1193 1269
Size: 1105 31 57 76
Fragments arranged by size:
1105 76 57 31
MspA1I CmG'CkG
Cuts at: 0 702 1269
Size: 702 567
NspI r_CATG'y
Cuts at: 0 1236 1269
Size: 1236 33
PasI CC'CwG_GG
Cuts at: 0 611 921 1269
Size: 611 310 348
Fragments arranged by size:
611 348 310
PciI A'CATG_T
Cuts at: 0 1232 1269
Size: 1232 37
PfoI T'CCnGG_A
Cuts at: 0 133 1269
Size: 133 1136
PpuMI rG'GwC_Cy
Cuts at: 0 16 390 1269
Size: 16 374 879
Fragments arranged by size:
879 374 16
PspOMI G'GGCC_C
Cuts at: 0 542 1269
Size: 542 727
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 99 131 1269
Size: 99 32 1138
Fragments arranged by size:
1138 99 32
PstI C_TGCA'G
Cuts at: 0 873 1269
Size: 873 396
PvuII CAG'CTG
Cuts at: 0 702 1269
Size: 702 567
SapI GCTCTTCn'nnn_
Cuts at: 0 808 1269
Size: 808 461
SexAI A'CCwGG_T
Cuts at: 0 959 1034 1269
Size: 959 75 235
Fragments arranged by size:
959 235 75
SfcI C'TryA_G
Cuts at: 0 36 869 1269
Size: 36 833 400
Fragments arranged by size:
833 400 36
SmaI CCC'GGG
Cuts at: 0 556 1269
Size: 556 713
SmlI C'TyrA_G
Cuts at: 0 1081 1269
Size: 1081 188
StyI C'CwwG_G
Cuts at: 0 101 458 679 1124 1269
Size: 101 357 221 445 145
Fragments arranged by size:
445 357 221 145 101
TatI w'GTAC_w
Cuts at: 0 250 1269
Size: 250 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5 37 135 167 1269
Size: 5 32 98 32 1102
Fragments arranged by size:
1102 98 32 32 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 918 1269
Size: 918 351
XmaI C'CCGG_G
Cuts at: 0 554 1269
Size: 554 715
XmnI GAAnn'nnTTC
Cuts at: 0 1066 1269
Size: 1066 203
Enzymes that do cut:
AcuI AflIII AlfI ApaI ApaLI AvaI BanII BbsI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BstXI BstYI Bsu36I BtgI BtsI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII MscI MslI MspA1I NspI
PasI PciI PfoI PpuMI PspOMI PsrI PstI PvuII
SapI SexAI SfcI SmaI SmlI StyI TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BsaAI BsaBI
BsgI BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FseI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
ZraI