Restriction map for pF1KB6634
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvrII       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiHKAI     BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MroI        MseI        MunI        NcoI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        ScaI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI          264  GTAC                 GT^AC
AflIII        176  ACACGT               A^C[GA][CT]GT
ApaI           15  GGGCCC               GGGCC^C
BamHI         629  GGATCC               G^GATCC
BbeI          445  GGCGCC               GGCGC^C
BpmI          450  CTGGAG               CTGGAG(16/14)
BseRI        1164  CTCCTC               GAGGAG(10/8)
BsmAI         703  GAGAC                GTCTC(1/5)
BsmBI         703  GAGACG               CGTCTC(1/5)
BsrBI         461  CCGCTC               CCGCTC(-3/-3)
BssHII        283  GCGCGC               G^CGCGC
BssSI         499  CACGAG               CACGAG(-5/-1)
BstXI         677  CCACCGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         629  GGATCC               [GA]^GATC[CT]
DsaI         1274  CCGCGG               C^C[GA][CT]GG
KasI          445  GGCGCC               G^GCGCC
KspI         1274  CCGCGG               CCGC^GG
MflI          629  GGATCC               [GA]^GATC[CT]
MslI          678  CACCGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          243  GCCGGC               GCC^GGC
NarI          445  GGCGCC               GG^CGCC
NdeI          609  CATATG               CA^TATG
NgoMIV        243  GCCGGC               G^CCGGC
NheI          636  GCTAGC               G^CTAGC
NspI          824  GCATGT               [GA]CATG^[CT]
PvuII        1189  CAGCTG               CAG^CTG
RsaI          264  GTAC                 GT^AC
SacII        1274  CCGCGG               CCGC^GG
SapI          201  GCTCTTC              GCTCTTC(1/4)
SexAI         838  ACCTGGT              A^CC[AT]GGT
SfoI          445  GGCGCC               GGC^GCC
TfiI         1135  GATTC                G^A[AT]TC
Tsp45I        835  GTGAC                ^GT[GC]AC
Tsp509I      1255  AATT                 ^AATT
XhoII         629  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI          630  GATCC                GGATC(4/5)
              629  GGATC               
BanII          15  GGGCCC               G[GA]GC[CT]^C
             1279  GAGCCC              
BsgI          153  CTGCAC               GTGCAG(16/14)
              658  GTGCAG              
BsiEI          89  CGGCCG               CG[GA][CT]^CG
             1291  CGGCCG              
BsrFI         243  GCCGGC               [GA]^CCGG[CT]
              679  ACCGGC              
BsrI          534  CCAGT                ACTGG(1/-1)
              689  CCAGT               
BstAPI        651  GCAGGCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1101  GCAGGTGCTGC         
Cfr10I        243  GCCGGC               [GA]^CCGG[CT]
              679  ACCGGC              
DpnII         141  GATC                 ^GATC
              630  GATC                
EagI           89  CGGCCG               C^GGCCG
             1291  CGGCCG              
EclXI          89  CGGCCG               C^GGCCG
             1291  CGGCCG              
Eco52I         89  CGGCCG               C^GGCCG
             1291  CGGCCG              
EcoNI         620  CCTACTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1095  CCTACAGCAGG         
MboI          141  GATC                 ^GATC
              630  GATC                
NdeII         141  GATC                 ^GATC
              630  GATC                
NotI           88  GCGGCCGC             GC^GGCCGC
             1290  GCGGCCGC            
PpuMI         721  AGGACCC              [GA]G^G[AT]CC[CT]
             1217  AGGACCC             
Sau3AI        141  GATC                 ^GATC
              630  GATC                
SmaI           66  CCCGGG               CCC^GGG
             1022  CCCGGG              
StuI          695  AGGCCT               AGG^CCT
              897  AGGCCT              
TspRI         535  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1055  CACTG               
XcmI          182  CCAACTTCTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              797  CCAGCCTGACGCTGG     
XmaCI          66  CCCGGG               C^CCGGG
             1022  CCCGGG              
XmaI           66  CCCGGG               C^CCGGG
             1022  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
AvaI        AvaII       AviII       BalI        BanI        
BbvI        BcnI        BfaI        BglI        BmyI        
BsaHI       BsaJI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboII       MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       PleI        PstI        Sau96I      
ScrFI       SfaNI       SfcI        SfiI        TaqI        
TseI        TthHB8I     
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