Restriction map for pF1KB6634
 (Linear) (Six-base) MAPSORT of: KIBB6634.seq  Check: 1693  from: 1  to: 1320



KIBB6634.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     651     702    1092    1320 
   Size:        651      51     390     228

  Fragments arranged by size: 

                 651     390     228      51
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1320 
   Size:        811     509
 
AflIII A'CryG_T

Cuts at:      0     176    1320 
   Size:        176    1144
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     649    1018    1320 
   Size:        649     369     302

  Fragments arranged by size: 

                 649     369     302
 
AlwNI CAG_nnn'CTG

Cuts at:      0      80    1107    1194    1320 
   Size:         80    1027      87     126

  Fragments arranged by size: 

                1027     126      87      80
 
ApaI G_GGCC'C

Cuts at:      0      19    1320 
   Size:         19    1301
 
AvaI C'yCGr_G

Cuts at:      0      66     600    1022    1167    1282    1320 
   Size:         66     534     422     145     115      38

  Fragments arranged by size: 

                 534     422     145     115      66      38
 
BamHI G'GATC_C

Cuts at:      0     629    1320 
   Size:        629     691
 
BanI G'GyrC_C

Cuts at:      0     445     604     714    1320 
   Size:        445     159     110     606

  Fragments arranged by size: 

                 606     445     159     110
 
BanII G_rGCy'C

Cuts at:      0      19    1283    1320 
   Size:         19    1264      37

  Fragments arranged by size: 

                1264      37      19
 
BbeI G_GCGC'C

Cuts at:      0     449    1320 
   Size:        449     871
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     694     793    1042    1320 
   Size:         46     648      99     249     278

  Fragments arranged by size: 

                 648     278     249      99      46
 
Bme1580I G_kGCm'C

Cuts at:      0      19     429     719    1320 
   Size:         19     410     290     601

  Fragments arranged by size: 

                 601     410     290      19
 
BmgBI CAC'GTC

Cuts at:      0     179     209     318    1320 
   Size:        179      30     109    1002

  Fragments arranged by size: 

                1002     179     109      30
 
BmtI G_CTAG'C

Cuts at:      0     640    1320 
   Size:        640     680
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471    1320 
   Size:        471     849
 
Bpu10I CC'TnA_GC

Cuts at:      0    1090    1155    1320 
   Size:       1090      65     165

  Fragments arranged by size: 

                1090     165      65
 
BsaHI Gr'CG_yC

Cuts at:      0     415     446     574     759    1320 
   Size:        415      31     128     185     561

  Fragments arranged by size: 

                 561     415     185     128      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     874     904    1320 
   Size:        874      30     416

  Fragments arranged by size: 

                 874     416      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1155    1320 
   Size:       1155     165
 
BseYI C'CCAG_C

Cuts at:      0      80     272     489     504     807     819     921     990 
   Size:         80     192     217      15     303      12     102      69

Cuts at:    990    1187    1320 
   Size:        197     133

  Fragments arranged by size: 

                 303     217     197     192     133     102      80      69
                  15      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     679    1320 
   Size:        138     541     641

  Fragments arranged by size: 

                 641     541     138
 
BsiEI CG_ry'CG

Cuts at:      0      92    1294    1320 
   Size:         92    1202      26

  Fragments arranged by size: 

                1202      92      26
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     697    1320 
   Size:        697     623
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     429     719    1283    1320 
   Size:         19     410     290     564      37

  Fragments arranged by size: 

                 564     410     290      37      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383     651     702     986    1092    1320 
   Size:        383     268      51     284     106     228

  Fragments arranged by size: 

                 383     284     268     228     106      51
 
BsrBI CCG'CTC

Cuts at:      0     463    1320 
   Size:        463     857
 
BsrFI r'CCGG_y

Cuts at:      0     243     679    1320 
   Size:        243     436     641

  Fragments arranged by size: 

                 641     436     243
 
BssHII G'CGCG_C

Cuts at:      0     283    1320 
   Size:        283    1037
 
BssSI C'ACGA_G

Cuts at:      0     499    1320 
   Size:        499     821
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     657    1107    1320 
   Size:        657     450     213

  Fragments arranged by size: 

                 657     450     213
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     684    1320 
   Size:        684     636
 
BstYI r'GATC_y

Cuts at:      0     629    1320 
   Size:        629     691
 
BtgI C'CryG_G

Cuts at:      0    1274    1320 
   Size:       1274      46
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      93    1320 
   Size:         93    1227
 
BtsI GCAGTG_nn'

Cuts at:      0    1054    1320 
   Size:       1054     266
 
EaeI y'GGCC_r

Cuts at:      0      89     125     245     517     531     548     686     857 
   Size:         89      36     120     272      14      17     138     171

Cuts at:    857    1291    1320 
   Size:        434      29

  Fragments arranged by size: 

                 434     272     171     138     120      89      36      29
                  17      14
 
EagI C'GGCC_G

Cuts at:      0      89    1291    1320 
   Size:         89    1202      29

  Fragments arranged by size: 

                1202      89      29
 
EarI CTCTTCn'nnn_

Cuts at:      0     208     907     970    1320 
   Size:        208     699      63     350

  Fragments arranged by size: 

                 699     350     208      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471     811    1320 
   Size:        471     340     509

  Fragments arranged by size: 

                 509     471     340
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     624    1099    1320 
   Size:        624     475     221

  Fragments arranged by size: 

                 624     475     221
 
EcoO109I rG'GnC_Cy

Cuts at:      0     722     786    1218    1320 
   Size:        722      64     432     102

  Fragments arranged by size: 

                 722     432     102      64
 
FspI TGC'GCA

Cuts at:      0     172     409    1060    1320 
   Size:        172     237     651     260

  Fragments arranged by size: 

                 651     260     237     172
 
HaeII r_GCGC'y

Cuts at:      0     273     335     449     991    1032    1320 
   Size:        273      62     114     542      41     288

  Fragments arranged by size: 

                 542     288     273     114      62      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     874     906    1126    1138    1158    1170    1320 
   Size:        874      32     220      12      20      12     150

  Fragments arranged by size: 

                 874     220     150      32      20      12      12
 
KasI G'GCGC_C

Cuts at:      0     445    1320 
   Size:        445     875
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1320 
   Size:        206    1114
 
MscI TGG'CCA

Cuts at:      0     533     550     688     859    1320 
   Size:        533      17     138     171     461

  Fragments arranged by size: 

                 533     461     171     138      17
 
MslI CAynn'nnrTG

Cuts at:      0     682    1320 
   Size:        682     638
 
MspA1I CmG'CkG

Cuts at:      0      77     393    1073    1113    1191    1227    1243    1276 
   Size:         77     316     680      40      78      36      16      33

Cuts at:   1276    1320 
   Size:         44

  Fragments arranged by size: 

                 680     316      78      77      44      40      36      33
                  16
 
NaeI GCC'GGC

Cuts at:      0     245    1320 
   Size:        245    1075
 
NarI GG'CG_CC

Cuts at:      0     446    1320 
   Size:        446     874
 
NdeI CA'TA_TG

Cuts at:      0     610    1320 
   Size:        610     710
 
NgoMIV G'CCGG_C

Cuts at:      0     243    1320 
   Size:        243    1077
 
NheI G'CTAG_C

Cuts at:      0     636    1320 
   Size:        636     684
 
NotI GC'GGCC_GC

Cuts at:      0      89    1291    1320 
   Size:         89    1202      29

  Fragments arranged by size: 

                1202      89      29
 
NspI r_CATG'y

Cuts at:      0     828    1320 
   Size:        828     492
 
PasI CC'CwG_GG

Cuts at:      0     733    1320 
   Size:        733     587
 
PpuMI rG'GwC_Cy

Cuts at:      0     722    1218    1320 
   Size:        722     496     102

  Fragments arranged by size: 

                 722     496     102
 
PspOMI G'GGCC_C

Cuts at:      0      15    1320 
   Size:         15    1305
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     247     279    1320 
   Size:        247      32    1041

  Fragments arranged by size: 

                1041     247      32
 
PstI C_TGCA'G

Cuts at:      0     331     628     713    1320 
   Size:        331     297      85     607

  Fragments arranged by size: 

                 607     331     297      85
 
PvuII CAG'CTG

Cuts at:      0    1191    1320 
   Size:       1191     129
 
SacII CC_GC'GG

Cuts at:      0    1277    1320 
   Size:       1277      43
 
SapI GCTCTTCn'nnn_

Cuts at:      0     208    1320 
   Size:        208    1112
 
SexAI A'CCwGG_T

Cuts at:      0     838    1320 
   Size:        838     482
 
SfcI C'TryA_G

Cuts at:      0     327     624     709    1096    1320 
   Size:        327     297      85     387     224

  Fragments arranged by size: 

                 387     327     297     224      85
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     694     793    1042    1320 
   Size:        694      99     249     278

  Fragments arranged by size: 

                 694     278     249      99
 
SfoI GGC'GCC

Cuts at:      0     447    1320 
   Size:        447     873
 
SmaI CCC'GGG

Cuts at:      0      68    1024    1320 
   Size:         68     956     296

  Fragments arranged by size: 

                 956     296      68
 
StuI AGG'CCT

Cuts at:      0     697     899    1320 
   Size:        697     202     421

  Fragments arranged by size: 

                 697     421     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      72     264    1228    1320 
   Size:         72     192     964      92

  Fragments arranged by size: 

                 964     192      92      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1292    1320 
   Size:       1292      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     189     804    1320 
   Size:        189     615     516

  Fragments arranged by size: 

                 615     516     189
 
XmaI C'CCGG_G

Cuts at:      0      66    1022    1320 
   Size:         66     956     298

  Fragments arranged by size: 

                 956     298      66
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbeI     BglI Bme1580I    BmgBI     BmtI     BpmI
   Bpu10I    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI     EarI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NspI     PasI    PpuMI   PspOMI     PsrI     PstI    PvuII    SacII
     SapI    SexAI     SfcI     SfiI     SfoI     SmaI     StuI    TaqII
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PvuI    RsrII     SacI     SalI    SanDI     SbfI
     ScaI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com