Restriction map for pF1KB6522
 (Linear) (Six-base) MAPSORT of: KIBB6522.seq  Check: 8184  from: 1  to: 1161



KIBB6522.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     755     925    1161 
   Size:        755     170     236

  Fragments arranged by size: 

                 755     236     170
 
ApoI r'AATT_y

Cuts at:      0     184     199     367     422     600     626    1161 
   Size:        184      15     168      55     178      26     535

  Fragments arranged by size: 

                 535     184     178     168      55      26      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     419    1161 
   Size:        419     742
 
BbvCI CC'TCA_GC

Cuts at:      0    1058    1161 
   Size:       1058     103
 
BclI T'GATC_A

Cuts at:      0     387    1161 
   Size:        387     774
 
BlpI GC'TnA_GC

Cuts at:      0     484    1161 
   Size:        484     677
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177    1161 
   Size:        177     984
 
Bpu10I CC'TnA_GC

Cuts at:      0    1058    1161 
   Size:       1058     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     857    1161 
   Size:        857     304
 
BsaAI yAC'GTr

Cuts at:      0     152     822    1097    1161 
   Size:        152     670     275      64

  Fragments arranged by size: 

                 670     275     152      64
 
BsaBI GATnn'nnATC

Cuts at:      0     926    1161 
   Size:        926     235
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25    1161 
   Size:         25    1136
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     545     565     955    1075    1161 
   Size:        545      20     390     120      86

  Fragments arranged by size: 

                 545     390     120      86      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419    1161 
   Size:        419     742
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     207    1161 
   Size:        207     954
 
BsrDI GCAATG_nn'

Cuts at:      0     283    1161 
   Size:        283     878
 
BsrGI T'GTAC_A

Cuts at:      0     810    1161 
   Size:        810     351
 
BssSI C'ACGA_G

Cuts at:      0     176    1161 
   Size:        176     985
 
BstYI r'GATC_y

Cuts at:      0      90     708    1161 
   Size:         90     618     453

  Fragments arranged by size: 

                 618     453      90
 
BtsI GCAGTG_nn'

Cuts at:      0     431    1161 
   Size:        431     730
 
DraI TTT'AAA

Cuts at:      0      86    1161 
   Size:         86    1075
 
EarI CTCTTCn'nnn_

Cuts at:      0     100     459     698     855    1161 
   Size:        100     359     239     157     306

  Fragments arranged by size: 

                 359     306     239     157     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177    1161 
   Size:        177     984
 
EcoO109I rG'GnC_Cy

Cuts at:      0     472     795    1161 
   Size:        472     323     366

  Fragments arranged by size: 

                 472     366     323
 
EcoRV GAT'ATC

Cuts at:      0     220    1032    1161 
   Size:        220     812     129

  Fragments arranged by size: 

                 812     220     129
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     111     247    1161 
   Size:        111     136     914

  Fragments arranged by size: 

                 914     136     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     204     236     601     633    1161 
   Size:         27     177      32     365      32     528

  Fragments arranged by size: 

                 528     365     177      32      32      27
 
HindIII A'AGCT_T

Cuts at:      0     440    1161 
   Size:        440     721
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1161 
   Size:        418     743
 
MslI CAynn'nnrTG

Cuts at:      0     562    1076    1161 
   Size:        562     514      85

  Fragments arranged by size: 

                 562     514      85
 
MspA1I CmG'CkG

Cuts at:      0     740    1161 
   Size:        740     421
 
PpuMI rG'GwC_Cy

Cuts at:      0     472    1161 
   Size:        472     689
 
PsiI TTA'TAA

Cuts at:      0     632    1161 
   Size:        632     529
 
PvuII CAG'CTG

Cuts at:      0     740    1161 
   Size:        740     421
 
SnaBI TAC'GTA

Cuts at:      0     152    1161 
   Size:        152    1009
 
SspI AAT'ATT

Cuts at:      0     649    1161 
   Size:        649     512
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     835    1161 
   Size:        835     326
 
TatI w'GTAC_w

Cuts at:      0     810    1161 
   Size:        810     351
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1131    1161 
   Size:       1131      30
 
XmnI GAAnn'nnTTC

Cuts at:      0      13    1161 
   Size:         13    1148
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BbvCI     BclI     BlpI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaXI    BseRI     BsgI    BsmBI    BsrDI
    BsrGI    BssSI    BstYI     BtsI     DraI     EarI  Eco57MI EcoO109I
    EcoRV     FalI    Hin4I  HindIII     MmeI     MslI   MspA1I    PpuMI
     PsiI    PvuII    SnaBI     SspI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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