Restriction map for pF1KE0677
 (Linear) (Six-base) MAPSORT of: KIEE0677.seq  Check: 2138  from: 1  to: 789



KIEE0677.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     789 
   Size:        109     680
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     414     446     789 
   Size:        414      32     343

  Fragments arranged by size: 

                 414     343      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     414     655     789 
   Size:        132     282     241     134

  Fragments arranged by size: 

                 282     241     134     132
 
ApaI G_GGCC'C

Cuts at:      0     532     789 
   Size:        532     257
 
AvaI C'yCGr_G

Cuts at:      0     142     789 
   Size:        142     647
 
BanI G'GyrC_C

Cuts at:      0     720     789 
   Size:        720      69
 
BanII G_rGCy'C

Cuts at:      0     197     314     532     789 
   Size:        197     117     218     257

  Fragments arranged by size: 

                 257     218     197     117
 
BbeI G_GCGC'C

Cuts at:      0     724     789 
   Size:        724      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     171     274     789 
   Size:        171     103     515

  Fragments arranged by size: 

                 515     171     103
 
BglI GCCn_nnn'nGGC

Cuts at:      0     616     718     789 
   Size:        616     102      71

  Fragments arranged by size: 

                 616     102      71
 
Bme1580I G_kGCm'C

Cuts at:      0      37     532     789 
   Size:         37     495     257

  Fragments arranged by size: 

                 495     257      37
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     211     789 
   Size:        211     578
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     418     789 
   Size:        320      98     371

  Fragments arranged by size: 

                 371     320      98
 
BsaHI Gr'CG_yC

Cuts at:      0     721     789 
   Size:        721      68
 
BsaWI w'CCGG_w

Cuts at:      0     107     789 
   Size:        107     682
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     318     337     348     367     789 
   Size:        318      19      11      19     422

  Fragments arranged by size: 

                 422     318      19      19      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     323     789 
   Size:        323     466
 
BseYI C'CCAG_C

Cuts at:      0     404     789 
   Size:        404     385
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     789 
   Size:        250     539
 
BsiHKAI G_wGCw'C

Cuts at:      0     197     541     647     789 
   Size:        197     344     106     142

  Fragments arranged by size: 

                 344     197     142     106
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     197     314     532     541     647     789 
   Size:         37     160     117     218       9     106     142

  Fragments arranged by size: 

                 218     160     142     117     106      37       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     222     789 
   Size:        222     567
 
BsrFI r'CCGG_y

Cuts at:      0     717     789 
   Size:        717      72
 
BtgI C'CryG_G

Cuts at:      0     314     560     789 
   Size:        314     246     229

  Fragments arranged by size: 

                 314     246     229
 
BtsI GCAGTG_nn'

Cuts at:      0      12      35     789 
   Size:         12      23     754

  Fragments arranged by size: 

                 754      23      12
 
EaeI y'GGCC_r

Cuts at:      0     482     608     617     789 
   Size:        482     126       9     172

  Fragments arranged by size: 

                 482     172     126       9
 
EarI CTCTTCn'nnn_

Cuts at:      0      63     128     185     775     789 
   Size:         63      65      57     590      14

  Fragments arranged by size: 

                 590      65      63      57      14
 
EcoICRI GAG'CTC

Cuts at:      0     195     789 
   Size:        195     594
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     320     418     789 
   Size:        109     211      98     371

  Fragments arranged by size: 

                 371     211     109      98
 
EcoO109I rG'GnC_Cy

Cuts at:      0     281     430     439     528     789 
   Size:        281     149       9      89     261

  Fragments arranged by size: 

                 281     261     149      89       9
 
FspI TGC'GCA

Cuts at:      0     628     789 
   Size:        628     161
 
HaeII r_GCGC'y

Cuts at:      0     102     397     724     789 
   Size:        102     295     327      65

  Fragments arranged by size: 

                 327     295     102      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     105     137     318     350     789 
   Size:        105      32     181      32     439

  Fragments arranged by size: 

                 439     181     105      32      32
 
KasI G'GCGC_C

Cuts at:      0     720     789 
   Size:        720      69
 
MscI TGG'CCA

Cuts at:      0     619     789 
   Size:        619     170
 
MspA1I CmG'CkG

Cuts at:      0     132     789 
   Size:        132     657
 
NaeI GCC'GGC

Cuts at:      0     719     789 
   Size:        719      70
 
NarI GG'CG_CC

Cuts at:      0     721     789 
   Size:        721      68
 
NcoI C'CATG_G

Cuts at:      0     314     560     789 
   Size:        314     246     229

  Fragments arranged by size: 

                 314     246     229
 
NgoMIV G'CCGG_C

Cuts at:      0     717     789 
   Size:        717      72
 
PasI CC'CwG_GG

Cuts at:      0     364     789 
   Size:        364     425
 
PpuMI rG'GwC_Cy

Cuts at:      0     430     789 
   Size:        430     359
 
PspOMI G'GGCC_C

Cuts at:      0     528     789 
   Size:        528     261
 
PstI C_TGCA'G

Cuts at:      0     438     665     789 
   Size:        438     227     124

  Fragments arranged by size: 

                 438     227     124
 
PvuII CAG'CTG

Cuts at:      0     132     789 
   Size:        132     657
 
SacI G_AGCT'C

Cuts at:      0     197     789 
   Size:        197     592
 
SapI GCTCTTCn'nnn_

Cuts at:      0     185     789 
   Size:        185     604
 
SbfI CC_TGCA'GG

Cuts at:      0     438     789 
   Size:        438     351
 
SfcI C'TryA_G

Cuts at:      0     434     661     789 
   Size:        434     227     128

  Fragments arranged by size: 

                 434     227     128
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     616     789 
   Size:        616     173
 
SfoI GGC'GCC

Cuts at:      0     722     789 
   Size:        722      67
 
SmlI C'TyrA_G

Cuts at:      0     142     789 
   Size:        142     647
 
StuI AGG'CCT

Cuts at:      0     499     789 
   Size:        499     290
 
StyI C'CwwG_G

Cuts at:      0     305     314     560     758     789 
   Size:        305       9     246     198      31

  Fragments arranged by size: 

                 305     246     198      31       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     312     789 
   Size:        312     477
 
XhoI C'TCGA_G

Cuts at:      0     142     789 
   Size:        142     647
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I     BplI     BpmI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrFI     BtgI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I     KasI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     PasI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI     SbfI
     SfcI     SfiI     SfoI     SmlI     StuI     StyI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MslI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     ScaI    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XmaI     XmnI     ZraI
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