Restriction map for pF1KE0713
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AvaI        AviII       AvrII       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        HphI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MseI        MspA1I      MunI        MvnI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SapI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        857  GGTACC               G^GTACC
AccIII        272  TCCGGA               T^CCGGA
AocI          560  CCTCAGG              CC^T[ACGT]AGG
Asp718I       857  GGTACC               G^GTACC
BamHI          41  GGATCC               G^GATCC
BbeI          115  GGCGCC               GGCGC^C
BglI          709  GCCTGCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         272  TCCGGA               [AT]^CCGG[AT]
BseAI         272  TCCGGA               T^CCGGA
BsgI          682  CTGCAC               GTGCAG(16/14)
BsmAI         457  GAGAC                GTCTC(1/5)
BspEI         272  TCCGGA               T^CCGGA
BspHI         807  TCATGA               T^CATGA
BsrI          735  CCAGT                ACTGG(1/-1)
BstAPI        684  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        151  GGATG                GGATG(2/0)
BstXI         474  CCATACTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          41  GGATCC               [GA]^GATC[CT]
Bsu36I        560  CCTCAGG              CC^T[ACGT]AGG
DpnII          42  GATC                 ^GATC
EarI          640  CTCTTC               CTCTTC(1/4)
Eco81I        560  CCTCAGG              CC^T[ACGT]AGG
EcoNI         576  CCTACGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          626  CCCGC                CCCGC(4/6)
FokI          151  GGATG                GGATG(9/13)
HapII         273  CCGG                 C^CGG
HgaI          539  GACGC                GACGC(5/10)
HpaII         273  CCGG                 C^CGG
KasI          115  GGCGCC               G^GCGCC
KpnI          857  GGTACC               GGTAC^C
Ksp632I       640  CTCTTC               CTCTTC(1/4)
MaeIII        849  GTCAC                ^GT[ACGT]AC
MboI           42  GATC                 ^GATC
MflI           41  GGATCC               [GA]^GATC[CT]
MroI          272  TCCGGA               T^CCGGA
MslI          645  CACTGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          273  CCGG                 C^CGG
NarI          115  GGCGCC               GG^CGCC
NdeII          42  GATC                 ^GATC
NspI          629  GCATGT               [GA]CATG^[CT]
PflMI         162  CCAAGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          241  CTGCAG               CTGCA^G
RcaI          807  TCATGA               T^CATGA
Sau3AI         42  GATC                 ^GATC
SbfI          240  CCTGCAGG             CCTGCA^GG
ScaI           29  AGTACT               AGT^ACT
SfaNI         152  GATGC                GCATC(5/9)
SfcI          241  CTGCAG               C^T[GA][CT]AG
SfoI          115  GGCGCC               GGC^GCC
Sse8387I      240  CCTGCAGG             CCTGCA^GG
TfiI          372  GAATC                G^A[AT]TC
Tsp45I        849  GTCAC                ^GT[GC]AC
Tsp509I       206  AATT                 ^AATT
Van91I        162  CCAAGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           81  CCAGGCCCTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          41  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI          115  GGCGCC               G[GA]^CG[CT]C
              539  GACGCC              
AlwI           42  GATCC                GGATC(4/5)
               41  GGATC               
BalI          662  TGGCCA               TGG^CCA
              759  TGGCCA              
BanII         103  GAGCTC               G[GA]GC[CT]^C
              193  GAGCTC              
BsaHI         115  GGCGCC               G[GA]^CG[CT]C
              539  GACGCC              
BseRI         655  CTCCTC               GAGGAG(10/8)
              841  GAGGAG              
BspMI         332  ACCTGC               ACCTGC(4/8)
              686  ACCTGC              
DsaI          609  CCATGG               C^C[GA][CT]GG
              728  CCATGG              
EaeI          662  TGGCCA               [CT]^GGCC[GA]
              759  TGGCCA              
Hin1I         115  GGCGCC               G[GA]^CG[CT]C
              539  GACGCC              
MluNI         662  TGGCCA               TGG^CCA
              759  TGGCCA              
MscI          662  TGGCCA               TGG^CCA
              759  TGGCCA              
NcoI          609  CCATGG               C^CATGG
              728  CCATGG              
PleI            8  GAGTC                GAGTC(4/5)
              470  GAGTC               
SacI          103  GAGCTC               GAGCT^C
              193  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BanI        BbvI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeII       HaeIII      HhaI        
HinP1I      HinfI       ItaI        MboII       MnlI        
MvaI        MwoI        NlaIII      NlaIV       PpuMI       
RsaI        Sau96I      ScrFI       StyI        TseI        
TspRI       
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