Restriction map for
pF1KE0713
(Circular) (Six-base) MAPSORT of: pF1KE0713.seq Check: 9971 from: 1 to: 4010
pF1KE0713 4010 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 803 803
Size: 4010
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 965 1026 965
Size: 61 3949
AclI AA'CG_TT
Cuts at: 1004 3850 1004
Size: 2846 1164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 412 1835 2267 3014 3282 382
Size: 30 1423 432 747 268 1110
Fragments arranged by size:
1423 1110 747 432 268 30
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AleI CACnn'nnGTG
Cuts at: 757 757
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 195 237 441 636 1315 2882 195
Size: 42 204 195 679 1567 1323
Fragments arranged by size:
1567 1323 679 204 195 42
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 949 957 982 990 949
Size: 8 25 8 3969
Fragments arranged by size:
3969 25 8 8
BamHI G'GATC_C
Cuts at: 149 1035 149
Size: 886 3124
BanI G'GyrC_C
Cuts at: 223 708 808 965 1026 1440 1688 1723 223
Size: 485 100 157 61 414 248 35 2510
Fragments arranged by size:
2510 485 414 248 157 100 61 35
BanII G_rGCy'C
Cuts at: 215 305 1024 2054 215
Size: 90 719 1030 2171
Fragments arranged by size:
2171 1030 719 90
BbeI G_GCGC'C
Cuts at: 227 1444 1692 227
Size: 1217 248 2545
Fragments arranged by size:
2545 1217 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3976
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglI GCCn_nnn'nGGC
Cuts at: 823 823
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 1635 1728 2784 1635
Size: 93 1056 2861
Fragments arranged by size:
2861 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 837 1222 1303 1638 3782 837
Size: 385 81 335 2144 1065
Fragments arranged by size:
2144 1065 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 418 451 298
Size: 120 33 3857
Fragments arranged by size:
3857 120 33
Bpu10I CC'TnA_GC
Cuts at: 182 182
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 224 648 1441 1689 2391 3887 224
Size: 424 793 248 702 1496 347
Fragments arranged by size:
1496 793 702 424 347 248
BsaWI w'CCGG_w
Cuts at: 380 1199 1720 2398 2672 2819 3230 380
Size: 819 521 678 274 147 411 1160
Fragments arranged by size:
1160 819 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 940 970 940
Size: 30 3980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 964 754
Size: 210 3800
BseYI C'CCAG_C
Cuts at: 111 263 1435 2193 2770 3266 111
Size: 152 1172 758 577 496 855
Fragments arranged by size:
1172 855 758 577 496 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 775
Size: 4010
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 215 305 764 1024 1802 1992 2784 215
Size: 90 459 260 778 190 792 1441
Fragments arranged by size:
1441 792 778 459 260 190 90
Bsp1286I G_dGCh'C
Cuts at: 215 305 764 1024 1635 1728 1802 1992 2054
Size: 90 459 260 611 93 74 190 62
Cuts at: 2054 2784 215
Size: 730 1441
Fragments arranged by size:
1441 730 611 459 260 190 93 90
74 62
BspEI T'CCGG_A
Cuts at: 380 3230 380
Size: 2850 1160
BspHI T'CATG_A
Cuts at: 915 3186 915
Size: 2271 1739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 803 1060 1274 1576 1957 449
Size: 354 257 214 302 381 2502
Fragments arranged by size:
2502 381 354 302 257 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 798 1387 798
Size: 589 3421
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstXI CCAn_nnnn'nTGG
Cuts at: 589 589
Size: 4010
BstYI r'GATC_y
Cuts at: 1 149 1035 1502 1525 1860 2106 3118 3204
Size: 148 886 467 23 335 246 1012 86
Cuts at: 3204 3216 3562 1
Size: 12 346 449
Fragments arranged by size:
1012 886 467 449 346 335 246 148
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 669 669
Size: 4010
BtgI C'CryG_G
Cuts at: 717 836 717
Size: 119 3891
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 787 1253 787
Size: 466 3544
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 770 867 1595 1769 2160 2187 3532 770
Size: 97 728 174 391 27 1345 1248
Fragments arranged by size:
1345 1248 728 391 174 97 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 754 2033 2243 754
Size: 1279 210 2521
Fragments arranged by size:
2521 1279 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 213 303 1022 213
Size: 90 719 3201
Fragments arranged by size:
3201 719 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 382 412 418 451 1835 2267 3014 3282
Size: 84 30 6 33 1384 432 747 268
Cuts at: 3282 298
Size: 1026
Fragments arranged by size:
1384 1026 747 432 268 84 33 30
6
EcoNI CCTnn'n_nnAGG
Cuts at: 688 688
Size: 4010
EcoO109I rG'GnC_Cy
Cuts at: 167 192 335 423 438 511 825 880 1149
Size: 25 143 88 15 73 314 55 269
Cuts at: 1149 167
Size: 3028
Fragments arranged by size:
3028 314 269 143 88 73 55 25
15
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 189 227 678 1444 1692 2714 3693 189
Size: 38 451 766 248 1022 979 506
Fragments arranged by size:
1022 979 766 506 451 248 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 1852 1882 1884 1914 940
Size: 32 880 30 2 30 3036
Fragments arranged by size:
3036 880 32 30 30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 223 1440 1688 223
Size: 1217 248 2545
Fragments arranged by size:
2545 1217 248
KpnI G_GTAC'C
Cuts at: 969 1030 969
Size: 61 3949
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 772 869 1771 772
Size: 97 902 3011
Fragments arranged by size:
3011 902 97
MslI CAynn'nnrTG
Cuts at: 757 1999 2126 757
Size: 1242 127 2641
Fragments arranged by size:
2641 1242 127
MspA1I CmG'CkG
Cuts at: 1121 1435 1795 2808 3053 1121
Size: 314 360 1013 245 2078
Fragments arranged by size:
2078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 224 1441 1689 224
Size: 1217 248 2545
Fragments arranged by size:
2545 1217 248
NcoI C'CATG_G
Cuts at: 717 836 717
Size: 119 3891
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 741 1063 1563 2470 741
Size: 322 500 907 2281
Fragments arranged by size:
2281 907 500 322
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PflMI CCAn_nnn'nTGG
Cuts at: 276 276
Size: 4010
PfoI T'CCnGG_A
Cuts at: 434 434
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PpuMI rG'GwC_Cy
Cuts at: 167 335 438 511 167
Size: 168 103 73 3666
Fragments arranged by size:
3666 168 103 73
PstI C_TGCA'G
Cuts at: 353 1057 1742 353
Size: 704 685 2621
Fragments arranged by size:
2621 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 215 305 1024 215
Size: 90 719 3201
Fragments arranged by size:
3201 719 90
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SanDI GG'GwC_CC
Cuts at: 167 167
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 353 1057 353
Size: 704 3306
ScaI AGT'ACT
Cuts at: 139 3527 139
Size: 3388 622
SfcI C'TryA_G
Cuts at: 33 349 1053 1738 2731 2922 33
Size: 316 704 685 993 191 1121
Fragments arranged by size:
1121 993 704 685 316 191
SfoI GGC'GCC
Cuts at: 225 1442 1690 225
Size: 1217 248 2545
Fragments arranged by size:
2545 1217 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 161 270 717 836 1144 1498 161
Size: 109 447 119 308 354 2673
Fragments arranged by size:
2673 447 354 308 119 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 137 3525 137
Size: 3388 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 196 1505 196
Size: 1309 2701
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbsI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI