Restriction map for pF1KE0713
 (Linear) (Six-base) MAPSORT of: KIEE0713.seq  Check: 604  from: 1  to: 897



KIEE0713.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     695     897 
   Size:        695     202
 
Acc65I G'GTAC_C

Cuts at:      0     857     897 
   Size:        857      40
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     304     897 
   Size:        274      30     593

  Fragments arranged by size: 

                 593     274      30
 
AleI CACnn'nnGTG

Cuts at:      0     649     897 
   Size:        649     248
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     129     333     528     897 
   Size:         87      42     204     195     369

  Fragments arranged by size: 

                 369     204     195      87      42
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     841     849     874     882     897 
   Size:        841       8      25       8      15

  Fragments arranged by size: 

                 841      25      15       8       8
 
BamHI G'GATC_C

Cuts at:      0      41     897 
   Size:         41     856
 
BanI G'GyrC_C

Cuts at:      0     115     600     700     857     897 
   Size:        115     485     100     157      40

  Fragments arranged by size: 

                 485     157     115     100      40
 
BanII G_rGCy'C

Cuts at:      0     107     197     897 
   Size:        107      90     700

  Fragments arranged by size: 

                 700     107      90
 
BbeI G_GCGC'C

Cuts at:      0     119     897 
   Size:        119     778
 
BglI GCCn_nnn'nGGC

Cuts at:      0     715     897 
   Size:        715     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     729     897 
   Size:        729     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     310     343     897 
   Size:        190     120      33     554

  Fragments arranged by size: 

                 554     190     120      33
 
Bpu10I CC'TnA_GC

Cuts at:      0      74     897 
   Size:         74     823
 
BsaHI Gr'CG_yC

Cuts at:      0     116     540     897 
   Size:        116     424     357

  Fragments arranged by size: 

                 424     357     116
 
BsaWI w'CCGG_w

Cuts at:      0     272     897 
   Size:        272     625
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     832     862     897 
   Size:        832      30      35

  Fragments arranged by size: 

                 832      35      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     646     856     897 
   Size:        646     210      41

  Fragments arranged by size: 

                 646     210      41
 
BseYI C'CCAG_C

Cuts at:      0       3     155     897 
   Size:          3     152     742

  Fragments arranged by size: 

                 742     152       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667     897 
   Size:        667     230
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     197     656     897 
   Size:        107      90     459     241

  Fragments arranged by size: 

                 459     241     107      90
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     197     656     897 
   Size:        107      90     459     241

  Fragments arranged by size: 

                 459     241     107      90
 
BspEI T'CCGG_A

Cuts at:      0     272     897 
   Size:        272     625
 
BspHI T'CATG_A

Cuts at:      0     807     897 
   Size:        807      90
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     341     695     897 
   Size:        341     354     202

  Fragments arranged by size: 

                 354     341     202
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     690     897 
   Size:        690     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     481     897 
   Size:        481     416
 
BstYI r'GATC_y

Cuts at:      0      41     897 
   Size:         41     856
 
Bsu36I CC'TnA_GG

Cuts at:      0     561     897 
   Size:        561     336
 
BtgI C'CryG_G

Cuts at:      0     609     728     897 
   Size:        609     119     169

  Fragments arranged by size: 

                 609     169     119
 
BtsI GCAGTG_nn'

Cuts at:      0     679     897 
   Size:        679     218
 
EaeI y'GGCC_r

Cuts at:      0     662     759     897 
   Size:        662      97     138

  Fragments arranged by size: 

                 662     138      97
 
EarI CTCTTCn'nnn_

Cuts at:      0     646     897 
   Size:        646     251
 
EcoICRI GAG'CTC

Cuts at:      0     105     195     897 
   Size:        105      90     702

  Fragments arranged by size: 

                 702     105      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     274     304     310     343     897 
   Size:        190      84      30       6      33     554

  Fragments arranged by size: 

                 554     190      84      33      30       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     580     897 
   Size:        580     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59      84     227     315     330     403     717     772 
   Size:         59      25     143      88      15      73     314      55

Cuts at:    772     897 
   Size:        125

  Fragments arranged by size: 

                 314     143     125      88      73      59      55      25
                  15
 
HaeII r_GCGC'y

Cuts at:      0      81     119     570     897 
   Size:         81      38     451     327

  Fragments arranged by size: 

                 451     327      81      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     832     864     897 
   Size:        832      32      33

  Fragments arranged by size: 

                 832      33      32
 
KasI G'GCGC_C

Cuts at:      0     115     897 
   Size:        115     782
 
KpnI G_GTAC'C

Cuts at:      0     861     897 
   Size:        861      36
 
MscI TGG'CCA

Cuts at:      0     664     761     897 
   Size:        664      97     136

  Fragments arranged by size: 

                 664     136      97
 
MslI CAynn'nnrTG

Cuts at:      0     649     897 
   Size:        649     248
 
NarI GG'CG_CC

Cuts at:      0     116     897 
   Size:        116     781
 
NcoI C'CATG_G

Cuts at:      0     609     728     897 
   Size:        609     119     169

  Fragments arranged by size: 

                 609     169     119
 
NspI r_CATG'y

Cuts at:      0     633     897 
   Size:        633     264
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     168     897 
   Size:        168     729
 
PfoI T'CCnGG_A

Cuts at:      0     326     897 
   Size:        326     571
 
PpuMI rG'GwC_Cy

Cuts at:      0      59     227     330     403     897 
   Size:         59     168     103      73     494

  Fragments arranged by size: 

                 494     168     103      73      59
 
PstI C_TGCA'G

Cuts at:      0     245     897 
   Size:        245     652
 
SacI G_AGCT'C

Cuts at:      0     107     197     897 
   Size:        107      90     700

  Fragments arranged by size: 

                 700     107      90
 
SanDI GG'GwC_CC

Cuts at:      0      59     897 
   Size:         59     838
 
SbfI CC_TGCA'GG

Cuts at:      0     245     897 
   Size:        245     652
 
ScaI AGT'ACT

Cuts at:      0      31     897 
   Size:         31     866
 
SfcI C'TryA_G

Cuts at:      0     241     897 
   Size:        241     656
 
SfoI GGC'GCC

Cuts at:      0     117     897 
   Size:        117     780
 
StyI C'CwwG_G

Cuts at:      0      53     162     609     728     897 
   Size:         53     109     447     119     169

  Fragments arranged by size: 

                 447     169     119     109      53
 
TatI w'GTAC_w

Cuts at:      0      29     897 
   Size:         29     868
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      88     897 
   Size:         88     809
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    AlwNI     BaeI    BamHI     BanI
    BanII     BbeI     BglI     BmrI     BpmI   Bpu10I    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI
     KpnI     MscI     MslI     NarI     NcoI     NspI    PflMI     PfoI
    PpuMI     PstI     SacI    SanDI     SbfI     ScaI     SfcI     SfoI
     StyI     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     MmeI   MspA1I     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI     SapI    SexAI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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