Restriction map for pF1KB6873
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MroI        MseI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp45I      Tth111I     XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AspHI          85  GAGCTC               G[AT]GC[AT]^C
AvaI          202  CCCGAG               C^[CT]CG[GA]G
BanI          488  GGCACC               G^G[CT][GA]CC
BanII          85  GAGCTC               G[GA]GC[CT]^C
BbsI          276  GAAGAC               GAAGAC(2/6)
BbvI          333  GCTGC                GCAGC(8/12)
BcnI           48  CCCGG                CC^[GC]GG
BmyI           85  GAGCTC               G[AGT]GC[ACT]^C
BpuAI         276  GAAGAC               GAAGAC(2/6)
BsgI          226  CTGCAC               GTGCAG(16/14)
BsiHKAI        85  GAGCTC               G[AT]GC[AT]^C
BsoBI         202  CCCGAG               C^[CT]CG[GA]G
Bsp1286I       85  GAGCTC               G[AGT]GC[ACT]^C
BsrI          411  CCAGT                ACTGG(1/-1)
BstAPI        401  GCATGATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI          523  GAAGAG               CTCTTC(1/4)
EcoNI         429  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI        333  GCTGC                GC^[ACGT]GC
HapII          49  CCGG                 C^CGG
HpaII          49  CCGG                 C^CGG
ItaI          333  GCTGC                GC^[ACGT]GC
Ksp632I       523  GAAGAG               CTCTTC(1/4)
MslI          117  CATGGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        246  CAGCTG               C[AC]G^C[GT]G
MspI           49  CCGG                 C^CGG
NciI           48  CCCGG                CC^[GC]GG
PflMI         411  CCAGTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          425  GAGTC                GAGTC(4/5)
PvuII         246  CAGCTG               CAG^CTG
SacI           85  GAGCTC               GAGCT^C
SapI          523  GAAGAGC              GCTCTTC(1/4)
SfcI          373  CTACAG               C^T[GA][CT]AG
TaqI          338  TCGA                 T^CGA
TseI          333  GCTGC                G^C[AT]GC
Tsp509I       355  AATT                 ^AATT
TthHB8I       338  TCGA                 T^CGA
Van91I        411  CCAGTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          414  GTAC                 GT^AC
              498  GTAC                
AluI           86  AGCT                 AG^CT
              247  AGCT                
AlwNI          76  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              412  CAGTACCTG           
BalI          119  TGGCCA               TGG^CCA
              419  TGGCCA              
BpmI           82  CTGGAG               CTGGAG(16/14)
              214  CTGGAG              
BsmFI         103  GGGAC                GGGAC(10/14)
               46  GTCCC               
BstF5I         60  CATCC                GGATG(2/0)
              393  CATCC               
BstXI         116  CCATGGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              422  CCAGAGTCCTGG        
DdeI          169  CTGAG                C^T[ACGT]AG
              281  CTGAG               
FokI           60  CATCC                GGATG(9/13)
              393  CATCC               
HphI           17  TCACC                GGTGA(8/7)
              182  TCACC               
MluNI         119  TGGCCA               TGG^CCA
              419  TGGCCA              
MscI          119  TGGCCA               TGG^CCA
              419  TGGCCA              
MwoI          319  GCGAACGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              401  GCATGATGTGC         
RsaI          414  GTAC                 GT^AC
              498  GTAC                
TfiI          285  GAATC                G^A[AT]TC
              458  GATTC               
TspRI         155  CACTG                CA[GC]TG[ACGT][ACGT]^
              188  CACTG               
XcmI           71  CCAAGCAGGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              422  CCAGAGTCCTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AvaII       BsaJI       BsiYI       BslI        BssKI       
BstNI       Cfr13I      DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     HaeIII      
HinfI       MboI        MboII       MnlI        MvaI        
NcoI        NdeII       NlaIII      NlaIV       PpuMI       
Sau3AI      Sau96I      ScrFI       StyI        
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