Restriction map for pF1KB6873
 (Linear) (Six-base) MAPSORT of: KIBB6873.seq  Check: 3906  from: 1  to: 540



KIBB6873.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     540 
   Size:        178     362
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     417     540 
   Size:         81     336     123

  Fragments arranged by size: 

                 336     123      81
 
AvaI C'yCGr_G

Cuts at:      0     202     540 
   Size:        202     338
 
BanI G'GyrC_C

Cuts at:      0     488     540 
   Size:        488      52
 
BanII G_rGCy'C

Cuts at:      0      89     540 
   Size:         89     451
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     283     540 
   Size:        283     257
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     235     540 
   Size:        103     132     305

  Fragments arranged by size: 

                 305     132     103
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     124     154     540 
   Size:        124      30     386

  Fragments arranged by size: 

                 386     124      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     540 
   Size:        211     329
 
BsiHKAI G_wGCw'C

Cuts at:      0      89     540 
   Size:         89     451
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     540 
   Size:         89     451
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     407     540 
   Size:        407     133
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     123     429     540 
   Size:        123     306     111

  Fragments arranged by size: 

                 306     123     111
 
BtgI C'CryG_G

Cuts at:      0      53     116     122     540 
   Size:         53      63       6     418

  Fragments arranged by size: 

                 418      63      53       6
 
EaeI y'GGCC_r

Cuts at:      0      50     119     419     540 
   Size:         50      69     300     121

  Fragments arranged by size: 

                 300     121      69      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     518     540 
   Size:        518      22
 
EcoICRI GAG'CTC

Cuts at:      0      87     540 
   Size:         87     453
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     178     235     540 
   Size:        103      75      57     305

  Fragments arranged by size: 

                 305     103      75      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     433     540 
   Size:        433     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     104     162     264     476     540 
   Size:         78      26      58     102     212      64

  Fragments arranged by size: 

                 212     102      78      64      58      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     270     302     540 
   Size:        270      32     238

  Fragments arranged by size: 

                 270     238      32
 
MscI TGG'CCA

Cuts at:      0     121     421     540 
   Size:        121     300     119

  Fragments arranged by size: 

                 300     121     119
 
MslI CAynn'nnrTG

Cuts at:      0     121     540 
   Size:        121     419
 
MspA1I CmG'CkG

Cuts at:      0     248     540 
   Size:        248     292
 
NcoI C'CATG_G

Cuts at:      0      53     116     122     540 
   Size:         53      63       6     418

  Fragments arranged by size: 

                 418      63      53       6
 
PasI CC'CwG_GG

Cuts at:      0      21     540 
   Size:         21     519
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     417     540 
   Size:        417     123
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     104     264     540 
   Size:         78      26     160     276

  Fragments arranged by size: 

                 276     160      78      26
 
PvuII CAG'CTG

Cuts at:      0     248     540 
   Size:        248     292
 
SacI G_AGCT'C

Cuts at:      0      89     540 
   Size:         89     451
 
SapI GCTCTTCn'nnn_

Cuts at:      0     518     540 
   Size:        518      22
 
SfcI C'TryA_G

Cuts at:      0     373     540 
   Size:        373     167
 
StyI C'CwwG_G

Cuts at:      0      53     116     122     267     540 
   Size:         53      63       6     145     273

  Fragments arranged by size: 

                 273     145      63      53       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     514     540 
   Size:        514      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      78     429     540 
   Size:         78     351     111

  Fragments arranged by size: 

                 351     111      78
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BanI    BanII     BbsI     BpmI    BsaXI
     BsgI  BsiHKAI Bsp1286I   BstAPI    BstXI     BtgI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I     MscI     MslI   MspA1I
     NcoI     PasI    PflMI    PpuMI    PvuII     SacI     SapI     SfcI
     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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