Restriction map for pF1KB7725
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AviII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrFI       BssHII      Bst1107I    BstAPI      BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRV       FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaII       KasI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I        310  GGTACC               G^GTACC
AflIII       1024  ACATGT               A^C[GA][CT]GT
AlwNI         693  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       310  GGTACC               G^GTACC
AspHI          76  GAGCTC               G[AT]GC[AT]^C
AvaII         159  GGACC                G^G[AT]CC
AvrII         244  CCTAGG               C^CTAGG
BanI          310  GGTACC               G^G[CT][GA]CC
BanII          76  GAGCTC               G[GA]GC[CT]^C
BbsI          457  GTCTTC               GAAGAC(2/6)
BcgI          366  GCATGTTTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          244  CCTAGG               C^CTAGG
BmyI           76  GAGCTC               G[AGT]GC[ACT]^C
BpuAI         457  GTCTTC               GAAGAC(2/6)
BsgI         1110  GTGCAG               GTGCAG(16/14)
BsiHKAI        76  GAGCTC               G[AT]GC[AT]^C
BsmFI         185  GGGAC                GGGAC(10/14)
Bsp1286I       76  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      179  TGTACA               T^GTACA
BspLU11I     1024  ACATGT               A^CATGT
BsrGI         179  TGTACA               T^GTACA
BssSI         885  CACGAG               CACGAG(-5/-1)
BstBI         374  TTCGAA               TT^CGAA
BstEII        105  GGTGACC              G^GT[ACGT]ACC
BstF5I        117  GGATG                GGATG(2/0)
BstPI         105  GGTGACC              G^GT[ACGT]ACC
BstXI         945  CCACGATTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          89  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        159  GGACC                G^G[ACGT]CC
DsaI          125  CCGTGG               C^C[GA][CT]GG
EaeI          122  TGGCCG               [CT]^GGCC[GA]
EarI          289  GAAGAG               CTCTTC(1/4)
EcoNI         962  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       105  GGTGACC              G^GT[ACGT]ACC
EcoT14I       244  CCTAGG               C^C[AT][AT]GG
EcoT22I      1252  ATGCAT               ATGCA^T
FauI         1205  GCGGG                CCCGC(4/6)
FokI          117  GGATG                GGATG(9/13)
HincII        351  GTTAAC               GT[CT]^[GA]AC
HindII        351  GTTAAC               GT[CT]^[GA]AC
HpaI          351  GTTAAC               GTT^AAC
KpnI          310  GGTACC               GGTAC^C
Ksp632I       289  GAAGAG               CTCTTC(1/4)
MaeII         188  ACGT                 A^CGT
MslI          946  CACGATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        259  CAGCTG               C[AC]G^C[GT]G
MwoI         1254  GCATAATAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI         1252  ATGCAT               ATGCA^T
NspV          374  TTCGAA               TT^CGAA
PvuII         259  CAGCTG               CAG^CTG
SacI           76  GAGCTC               GAGCT^C
Sau96I        159  GGACC                G^G[ACGT]CC
SfaNI         192  GATGC                GCATC(5/9)
SfuI          374  TTCGAA               TT^CGAA
SmlI          741  CTTGAG               C^T[CT][GA]AG
SspBI         179  TGTACA               T^GTACA
StyI          244  CCTAGG               C^C[AT][AT]GG
XbaI          388  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          299  GAATTC               [GA]^AATT[CT]
              746  GAATTC              
ApoI          299  GAATTC               [GA]^AATT[CT]
              746  GAATTC              
BfaI          245  CTAG                 C^TAG
              389  CTAG                
BglII         553  AGATCT               A^GATCT
              637  AGATCT              
BseRI         142  GAGGAG               GAGGAG(10/8)
              969  GAGGAG              
BsrDI         152  CATTGC               GCAATG(2/0)
              608  GCAATG              
BssKI         138  CCAGG                ^CC[ACGT]GG
              213  CCTGG               
BstNI         138  CCAGG                CC^[AT]GG
              213  CCTGG               
BstYI         553  AGATCT               [GA]^GATC[CT]
              637  AGATCT              
DraI          337  TTTAAA               TTT^AAA
              451  TTTAAA              
EcoRI         299  GAATTC               G^AATTC
              746  GAATTC              
EcoRII        138  CCAGG                ^CC[AT]GG
              213  CCTGG               
HaeIII        123  GGCC                 GG^CC
              216  GGCC                
HphI          105  GGTGA                GGTGA(8/7)
              134  TCACC               
MaeI          245  CTAG                 C^TAG
              389  CTAG                
MflI          553  AGATCT               [GA]^GATC[CT]
              637  AGATCT              
MvaI          138  CCAGG                CC^[AT]GG
              213  CCTGG               
NspI          366  GCATGT               [GA]CATG^[CT]
             1024  ACATGT              
PstI          204  CTGCAG               CTGCA^G
              522  CTGCAG              
ScrFI         138  CCAGG                CC^[ACGT]GG
              213  CCTGG               
TaqI          375  TCGA                 T^CGA
              976  TCGA                
Tsp45I        106  GTGAC                ^GT[GC]AC
              695  GTGAC               
TthHB8I       375  TCGA                 T^CGA
              976  TCGA                
XhoII         553  AGATCT               [GA]^GATC[CT]
              637  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AfaI        AluI        Asp700I     BbvI        
BpmI        BsaJI       BsiYI       BslI        BsmI        
BsrI        DdeI        DpnII       Fnu4HI      HinfI       
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      SfcI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XmnI        
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