Restriction map for pF1KB7725
 (Linear) (Six-base) MAPSORT of: KIBB7725.seq  Check: 2968  from: 1  to: 1269



KIBB7725.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

Acc65I G'GTAC_C

Cuts at:      0     310    1269 
   Size:        310     959
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     612     696     948    1269 
   Size:        444     168      84     252     321

  Fragments arranged by size: 

                 444     321     252     168      84
 
AflIII A'CryG_T

Cuts at:      0    1024    1269 
   Size:       1024     245
 
AleI CACnn'nnGTG

Cuts at:      0     950    1269 
   Size:        950     319
 
AlwNI CAG_nnn'CTG

Cuts at:      0     698    1269 
   Size:        698     571
 
ApoI r'AATT_y

Cuts at:      0     299     746    1269 
   Size:        299     447     523

  Fragments arranged by size: 

                 523     447     299
 
AvrII C'CTAG_G

Cuts at:      0     244    1269 
   Size:        244    1025
 
BanI G'GyrC_C

Cuts at:      0     310    1269 
   Size:        310     959
 
BanII G_rGCy'C

Cuts at:      0      80    1269 
   Size:         80    1189
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     450    1269 
   Size:        450     819
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     355     389    1269 
   Size:        355      34     880

  Fragments arranged by size: 

                 880     355      34
 
BfrBI ATG'CAT

Cuts at:      0    1254    1269 
   Size:       1254      15
 
BglII A'GATC_T

Cuts at:      0     553     637    1269 
   Size:        553      84     632

  Fragments arranged by size: 

                 632     553      84
 
BmgBI CAC'GTC

Cuts at:      0     189    1269 
   Size:        189    1080
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     232    1269 
   Size:        232    1037
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     319     445     911    1269 
   Size:        319     126     466     358

  Fragments arranged by size: 

                 466     358     319     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     283     524     776    1028    1269 
   Size:        217      66     241     252     252     241

  Fragments arranged by size: 

                 252     252     241     241     217      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     359    1269 
   Size:        359     910
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762    1269 
   Size:        762     507
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      53      83    1269 
   Size:         53      30    1186

  Fragments arranged by size: 

                1186      53      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     984    1269 
   Size:        157     827     285

  Fragments arranged by size: 

                 827     285     157
 
BseYI C'CCAG_C

Cuts at:      0     257    1269 
   Size:        257    1012
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1131    1269 
   Size:       1131     138
 
BsiHKAI G_wGCw'C

Cuts at:      0      80    1269 
   Size:         80    1189
 
BsmI GAATG_Cn'

Cuts at:      0     610     694     914     946    1256    1269 
   Size:        610      84     220      32     310      13

  Fragments arranged by size: 

                 610     310     220      84      32      13
 
Bsp1286I G_dGCh'C

Cuts at:      0      80    1269 
   Size:         80    1189
 
BsrDI GCAATG_nn'

Cuts at:      0     151     615    1269 
   Size:        151     464     654

  Fragments arranged by size: 

                 654     464     151
 
BsrGI T'GTAC_A

Cuts at:      0     179    1269 
   Size:        179    1090
 
BssSI C'ACGA_G

Cuts at:      0     885    1269 
   Size:        885     384
 
BstBI TT'CG_AA

Cuts at:      0     375    1269 
   Size:        375     894
 
BstEII G'GTnAC_C

Cuts at:      0     105    1269 
   Size:        105    1164
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     952    1269 
   Size:        952     317
 
BstYI r'GATC_y

Cuts at:      0     553     637    1269 
   Size:        553      84     632

  Fragments arranged by size: 

                 632     553      84
 
BtgI C'CryG_G

Cuts at:      0     125    1269 
   Size:        125    1144
 
BtsI GCAGTG_nn'

Cuts at:      0     531     699    1119    1269 
   Size:        531     168     420     150

  Fragments arranged by size: 

                 531     420     168     150
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     597     632    1269 
   Size:        597      35     637

  Fragments arranged by size: 

                 637     597      35
 
DraI TTT'AAA

Cuts at:      0     339     453    1269 
   Size:        339     114     816

  Fragments arranged by size: 

                 816     339     114
 
EaeI y'GGCC_r

Cuts at:      0     122    1269 
   Size:        122    1147
 
EarI CTCTTCn'nnn_

Cuts at:      0     284    1269 
   Size:        284     985
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      74    1269 
   Size:         74    1195
 
EcoICRI GAG'CTC

Cuts at:      0      78    1269 
   Size:         78    1191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     283     444     524     612     696     776     948 
   Size:        217      66     161      80      88      84      80     172

Cuts at:    948    1028    1269 
   Size:         80     241

  Fragments arranged by size: 

                 241     217     172     161      88      84      80      80
                  80      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     966    1269 
   Size:        966     303
 
EcoRI G'AATT_C

Cuts at:      0     299     746    1269 
   Size:        299     447     523

  Fragments arranged by size: 

                 523     447     299
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     399     431     963     995    1269 
   Size:        399      32     532      32     274

  Fragments arranged by size: 

                 532     399     274      32      32
 
HincII GTy'rAC

Cuts at:      0     353    1269 
   Size:        353     916
 
HpaI GTT'AAC

Cuts at:      0     353    1269 
   Size:        353     916
 
KpnI G_GTAC'C

Cuts at:      0     314    1269 
   Size:        314     955
 
MslI CAynn'nnrTG

Cuts at:      0     950    1269 
   Size:        950     319
 
MspA1I CmG'CkG

Cuts at:      0     261    1269 
   Size:        261    1008
 
NsiI A_TGCA'T

Cuts at:      0    1256    1269 
   Size:       1256      13
 
NspI r_CATG'y

Cuts at:      0     370    1028    1269 
   Size:        370     658     241

  Fragments arranged by size: 

                 658     370     241
 
PciI A'CATG_T

Cuts at:      0    1024    1269 
   Size:       1024     245
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234    1269 
   Size:        202      32    1035

  Fragments arranged by size: 

                1035     202      32
 
PstI C_TGCA'G

Cuts at:      0     208     526    1269 
   Size:        208     318     743

  Fragments arranged by size: 

                 743     318     208
 
PvuII CAG'CTG

Cuts at:      0     261    1269 
   Size:        261    1008
 
SacI G_AGCT'C

Cuts at:      0      80    1269 
   Size:         80    1189
 
SfcI C'TryA_G

Cuts at:      0     204     522     543     909    1269 
   Size:        204     318      21     366     360

  Fragments arranged by size: 

                 366     360     318     204      21
 
SmlI C'TyrA_G

Cuts at:      0     741    1269 
   Size:        741     528
 
StyI C'CwwG_G

Cuts at:      0     244    1269 
   Size:        244    1025
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     151    1269 
   Size:        151    1118
 
TatI w'GTAC_w

Cuts at:      0     179    1269 
   Size:        179    1090
 
XbaI T'CTAG_A

Cuts at:      0     388    1269 
   Size:        388     881
 
XmnI GAAnn'nnTTC

Cuts at:      0     625     709    1213    1269 
   Size:        625      84     504      56

  Fragments arranged by size: 

                 625     504      84      56
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI    AlwNI     ApoI    AvrII     BanI
    BanII     BbsI     BcgI    BfrBI    BglII    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI
    BstYI     BtgI     BtsI    CspCI     DraI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    Hin4I   HincII     HpaI     KpnI
     MslI   MspA1I     NsiI     NspI     PciI     PpiI     PstI    PvuII
     SacI     SfcI     SmlI     StyI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI     BglI     BlpI
 Bme1580I     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
   BstAPI  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     DrdI     EagI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XcmI     XhoI     XmaI     ZraI
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