Restriction map for pF1KB6516
 (Linear) (Six-base) MAPSORT of: KIBB6516.seq  Check: 2913  from: 1  to: 1155



KIBB6516.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     583    1155 
   Size:        583     572
 
Acc65I G'GTAC_C

Cuts at:      0     655    1155 
   Size:        655     500
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     672     678     696     743     858    1155 
   Size:        383     289       6      18      47     115     297

  Fragments arranged by size: 

                 383     297     289     115      47      18       6
 
ApaI G_GGCC'C

Cuts at:      0     605    1155 
   Size:        605     550
 
BanI G'GyrC_C

Cuts at:      0     655    1155 
   Size:        655     500
 
BanII G_rGCy'C

Cuts at:      0     395     605    1155 
   Size:        395     210     550

  Fragments arranged by size: 

                 550     395     210
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     277    1155 
   Size:        277     878
 
BglII A'GATC_T

Cuts at:      0     428    1155 
   Size:        428     727
 
Bme1580I G_kGCm'C

Cuts at:      0     605    1155 
   Size:        605     550
 
BmgBI CAC'GTC

Cuts at:      0     335    1155 
   Size:        335     820
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     387     600     749    1155 
   Size:        387     213     149     406

  Fragments arranged by size: 

                 406     387     213     149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     770    1155 
   Size:        286     484     385

  Fragments arranged by size: 

                 484     385     286
 
BsaBI GATnn'nnATC

Cuts at:      0     307     433    1155 
   Size:        307     126     722

  Fragments arranged by size: 

                 722     307     126
 
BsaHI Gr'CG_yC

Cuts at:      0     473    1155 
   Size:        473     682
 
BsaWI w'CCGG_w

Cuts at:      0     311    1155 
   Size:        311     844
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     536     566    1155 
   Size:        536      30     589

  Fragments arranged by size: 

                 589     536      30
 
BseYI C'CCAG_C

Cuts at:      0     651    1155 
   Size:        651     504
 
BsiHKAI G_wGCw'C

Cuts at:      0      67    1155 
   Size:         67    1088
 
BsmI GAATG_Cn'

Cuts at:      0    1069    1155 
   Size:       1069      86
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     395     605    1155 
   Size:         67     328     210     550

  Fragments arranged by size: 

                 550     328     210      67
 
BspEI T'CCGG_A

Cuts at:      0     311    1155 
   Size:        311     844
 
BspHI T'CATG_A

Cuts at:      0     845    1155 
   Size:        845     310
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     583    1155 
   Size:        583     572
 
BstYI r'GATC_y

Cuts at:      0     428    1155 
   Size:        428     727
 
BtgI C'CryG_G

Cuts at:      0      49     540     659    1155 
   Size:         49     491     119     496

  Fragments arranged by size: 

                 496     491     119      49
 
BtsI GCAGTG_nn'

Cuts at:      0     540    1155 
   Size:        540     615
 
DraIII CAC_nnn'GTG

Cuts at:      0     706    1155 
   Size:        706     449
 
EaeI y'GGCC_r

Cuts at:      0     537     866    1155 
   Size:        537     329     289

  Fragments arranged by size: 

                 537     329     289
 
EarI CTCTTCn'nnn_

Cuts at:      0    1045    1155 
   Size:       1045     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     265    1155 
   Size:        265     890
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     383     672     678     696     743     770     858 
   Size:        286      97     289       6      18      47      27      88

Cuts at:    858    1155 
   Size:        297

  Fragments arranged by size: 

                 297     289     286      97      88      47      27      18
                   6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     602     642    1155 
   Size:        602      40     513

  Fragments arranged by size: 

                 602     513      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     808     840    1155 
   Size:        808      32     315

  Fragments arranged by size: 

                 808     315      32
 
KpnI G_GTAC'C

Cuts at:      0     659    1155 
   Size:        659     496
 
MscI TGG'CCA

Cuts at:      0     539     868    1155 
   Size:        539     329     287

  Fragments arranged by size: 

                 539     329     287
 
MslI CAynn'nnrTG

Cuts at:      0      54    1048    1155 
   Size:         54     994     107

  Fragments arranged by size: 

                 994     107      54
 
MspA1I CmG'CkG

Cuts at:      0     716    1155 
   Size:        716     439
 
NcoI C'CATG_G

Cuts at:      0      49     540     659    1155 
   Size:         49     491     119     496

  Fragments arranged by size: 

                 496     491     119      49
 
NspI r_CATG'y

Cuts at:      0     893    1155 
   Size:        893     262
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     185     864    1155 
   Size:        185     679     291

  Fragments arranged by size: 

                 679     291     185
 
PsiI TTA'TAA

Cuts at:      0    1031    1155 
   Size:       1031     124
 
PspOMI G'GGCC_C

Cuts at:      0     601    1155 
   Size:        601     554
 
PstI C_TGCA'G

Cuts at:      0      10    1155 
   Size:         10    1145
 
PvuII CAG'CTG

Cuts at:      0     716    1155 
   Size:        716     439
 
SfcI C'TryA_G

Cuts at:      0       6     852    1155 
   Size:          6     846     303

  Fragments arranged by size: 

                 846     303       6
 
StyI C'CwwG_G

Cuts at:      0      49     540     659    1155 
   Size:         49     491     119     496

  Fragments arranged by size: 

                 496     491     119      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     327    1155 
   Size:         82     245     828

  Fragments arranged by size: 

                 828     245      82
 
TatI w'GTAC_w

Cuts at:      0     667    1155 
   Size:        667     488
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     773    1155 
   Size:        773     382
 
XmnI GAAnn'nnTTC

Cuts at:      0      88    1155 
   Size:         88    1067
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     ApaI     BanI    BanII     BbsI    BglII
 Bme1580I    BmgBI     BmrI     BpmI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BstYI
     BtgI     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I
    Hin4I     KpnI     MscI     MslI   MspA1I     NcoI     NspI    PflMI
     PsiI   PspOMI     PstI    PvuII     SfcI     StyI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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