Restriction map for pF1KB6893
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          412  GTCGAC               GT^[AC][GT]AC
AfaI          360  GTAC                 GT^AC
AflIII        106  ACATGT               A^C[GA][CT]GT
AlwI          277  GGATC                GGATC(4/5)
Asp700I       334  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          21  GTGCTC               G[AT]GC[AT]^C
AvaI          355  CTCGAG               C^[CT]CG[GA]G
AvaII         170  GGACC                G^G[AT]CC
BbsI          385  GAAGAC               GAAGAC(2/6)
BbvI          508  GCTGC                GCAGC(8/12)
BfaI          548  CTAG                 C^TAG
BmyI           21  GTGCTC               G[AGT]GC[ACT]^C
BpuAI         385  GAAGAC               GAAGAC(2/6)
BsaI          283  GGTCTC               GGTCTC(1/5)
BsaJI         288  CCTGGG               C^C[ACGT][ACGT]GG
BsiHKAI        21  GTGCTC               G[AT]GC[AT]^C
BsmAI         284  GTCTC                GTCTC(1/5)
BsmFI         180  GGGAC                GGGAC(10/14)
BsoBI         355  CTCGAG               C^[CT]CG[GA]G
Bsp1286I       21  GTGCTC               G[AGT]GC[ACT]^C
BspLU11I      106  ACATGT               A^CATGT
BsrDI         424  CATTGC               GCAATG(2/0)
BsrI          325  ACTGG                ACTGG(1/-1)
CfoI          455  GCGC                 GCG^C
Cfr13I        170  GGACC                G^G[ACGT]CC
DpnII         278  GATC                 ^GATC
DraI          265  TTTAAA               TTT^AAA
Fnu4HI        508  GCTGC                GC^[ACGT]GC
HaeIII        352  GGCC                 GG^CC
HhaI          455  GCGC                 GCG^C
HinP1I        455  GCGC                 G^CGC
ItaI          508  GCTGC                GC^[ACGT]GC
MaeI          548  CTAG                 C^TAG
MboI          278  GATC                 ^GATC
MspA1I        506  CAGCTG               C[AC]G^C[GT]G
NdeII         278  GATC                 ^GATC
PaeR7I        355  CTCGAG               C^TCGAG
PleI          415  GACTC                GAGTC(4/5)
PvuII         506  CAGCTG               CAG^CTG
RsaI          360  GTAC                 GT^AC
SalI          412  GTCGAC               G^TCGAC
Sau3AI        278  GATC                 ^GATC
Sau96I        170  GGACC                G^G[ACGT]CC
SfaNI         392  GCATC                GCATC(5/9)
SmlI          355  CTCGAG               C^T[CT][GA]AG
SphI           54  GCATGC               GCATG^C
SspI           35  AATATT               AAT^ATT
StuI          351  AGGCCT               AGG^CCT
TfiI          162  GATTC                G^A[AT]TC
TseI          508  GCTGC                G^C[AT]GC
XhoI          355  CTCGAG               C^TCGAG
XmnI          334  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BseRI          95  CTCCTC               GAGGAG(10/8)
               98  CTCCTC              
BsiYI         288  CCTGGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              438  CCCAGCTATGG         
BslI          288  CCTGGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              438  CCCAGCTATGG         
BsmI          156  GAATGC               GAATGC(1/-1)
              373  GAATGC              
BssKI         288  CCTGG                ^CC[ACGT]GG
              309  CCTGG               
BstNI         288  CCTGG                CC^[AT]GG
              309  CCTGG               
DdeI          184  CTGAG                C^T[ACGT]AG
              229  CTAAG               
EcoRII        288  CCTGG                ^CC[AT]GG
              309  CCTGG               
HincII         71  GTCAAC               GT[CT]^[GA]AC
              412  GTCGAC              
HindII         71  GTCAAC               GT[CT]^[GA]AC
              412  GTCGAC              
HinfI         162  GATTC                G^A[ACGT]TC
              415  GACTC               
HphI          328  GGTGA                GGTGA(8/7)
              470  TCACC               
MaeIII        133  GTGAC                ^GT[ACGT]AC
              469  GTCAC               
MvaI          288  CCTGG                CC^[AT]GG
              309  CCTGG               
MwoI          392  GCATCAAGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              511  GCTTTGAGTGC         
NspI           54  GCATGC               [GA]CATG^[CT]
              106  ACATGT              
ScrFI         288  CCTGG                CC^[ACGT]GG
              309  CCTGG               
Tsp45I        133  GTGAC                ^GT[GC]AC
              469  GTCAC               
TspRI          19  CAGTG                CA[GC]TG[ACGT][ACGT]^
              143  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        Cac8I       MboII       MnlI        MseI        
NlaIII      TaqI        Tru9I       Tsp509I     TthHB8I     
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