Restriction map for pF1KB6820
##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssKI       Bst1107I    BstAPI      BstEII      
BstNI       BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT22I     FbaI        FseI        
FspI        HaeIII      HapII       Hin1I       HincII      
HindII      HpaI        HpaII       HphI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MspI        MunI        MvaI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau96I      SbfI        ScrFI       
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         120  CGCG                 CG^CG
AfaI           87  GTAC                 GT^AC
AvaI           90  CTCGGG               C^[CT]CG[GA]G
BsaJI         410  CCTTGG               C^C[ACGT][ACGT]GG
BseRI         260  GAGGAG               GAGGAG(10/8)
BsmFI         209  GGGAC                GGGAC(10/14)
BsmI          537  GCATTC               GAATGC(1/-1)
BsoBI          90  CTCGGG               C^[CT]CG[GA]G
BsrBI         247  GAGCGG               CCGCTC(-3/-3)
BssSI          73  CTCGTG               CACGAG(-5/-1)
BstBI         233  TTCGAA               TT^CGAA
BstF5I        414  GGATG                GGATG(2/0)
BstUI         120  CGCG                 CG^CG
CfoI            7  GCGC                 GCG^C
EcoRI         365  GAATTC               G^AATTC
EcoRV         511  GATATC               GAT^ATC
EcoT14I       410  CCTTGG               C^C[AT][AT]GG
FauI           17  CCCGC                CCCGC(4/6)
FokI          414  GGATG                GGATG(9/13)
HaeII           6  GGCGCT               [GA]GCGC^[CT]
HhaI            7  GCGC                 GCG^C
HinP1I          7  GCGC                 G^CGC
HindIII       314  AAGCTT               A^AGCTT
MaeII         191  ACGT                 A^CGT
MslI          518  CACTTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          120  CGCG                 CG^CG
NlaIV          32  GGTTCC               GG[ACGT]^[ACGT]CC
NspI          239  GCATGT               [GA]CATG^[CT]
NspV          233  TTCGAA               TT^CGAA
PleI          263  GAGTC                GAGTC(4/5)
RsaI           87  GTAC                 GT^AC
SapI           42  GCTCTTC              GCTCTTC(1/4)
ScaI           86  AGTACT               AGT^ACT
SfaNI         289  GATGC                GCATC(5/9)
SfuI          233  TTCGAA               TT^CGAA
StyI          410  CCTTGG               C^C[AT][AT]GG
TaqI          234  TCGA                 T^CGA
TfiI          441  GAATC                G^A[AT]TC
Tsp45I        193  GTCAC                ^GT[GC]AC
TthHB8I       234  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI           18  CCGC                 CCGC(-3/-1)
              249  GCGG                
AcsI          365  GAATTC               [GA]^AATT[CT]
              467  AAATTT              
ApoI          365  GAATTC               [GA]^AATT[CT]
              467  AAATTT              
BglII         203  AGATCT               A^GATCT
              396  AGATCT              
BsiYI          18  CCGCCTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              151  CCCACAGTGGG         
BslI           18  CCGCCTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              151  CCCACAGTGGG         
BsrI           29  ACTGG                ACTGG(1/-1)
              370  CCAGT               
BstYI         203  AGATCT               [GA]^GATC[CT]
              396  AGATCT              
Cac8I           3  GCTGGC               GC[ACGT]^[ACGT]GC
               20  GCCTGC              
EarI           43  CTCTTC               CTCTTC(1/4)
               55  GAAGAG              
HgaI           69  GACGC                GACGC(5/10)
              121  GCGTC               
HinfI         263  GAGTC                G^A[ACGT]TC
              441  GAATC               
Ksp632I        43  CTCTTC               CTCTTC(1/4)
               55  GAAGAG              
MflI          203  AGATCT               [GA]^GATC[CT]
              396  AGATCT              
MwoI           72  GCTCGTGGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              528  GCTTATTCAGC         
PstI           82  CTGCAG               CTGCA^G
              293  CTGCAG              
SfcI           82  CTGCAG               C^T[GA][CT]AG
              293  CTGCAG              
XbaI          342  TCTAGA               T^CTAGA
              547  TCTAGA              
XhoII         203  AGATCT               [GA]^GATC[CT]
              396  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BbvI        BfaI        DpnII       Fnu4HI      
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        NdeII       NlaIII      Sau3AI      TseI        
Tsp509I     TspRI       
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