Restriction map for pF1KB6796
 (Linear) (Six-base) MAPSORT of: KIBB6796.seq  Check: 6968  from: 1  to: 468



KIBB6796.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     193     468 
   Size:        193     275
 
AlwNI CAG_nnn'CTG

Cuts at:      0     126     468 
   Size:        126     342
 
ApoI r'AATT_y

Cuts at:      0     104     468 
   Size:        104     364
 
AvaI C'yCGr_G

Cuts at:      0      43     468 
   Size:         43     425
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      37     467     468 
   Size:         37     430       1

  Fragments arranged by size: 

                 430      37       1
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      54     468 
   Size:         54     414
 
BclI T'GATC_A

Cuts at:      0     114     468 
   Size:        114     354
 
Bme1580I G_kGCm'C

Cuts at:      0     364     468 
   Size:        364     104
 
BseYI C'CCAG_C

Cuts at:      0     382     468 
   Size:        382      86
 
Bsp1286I G_dGCh'C

Cuts at:      0     364     468 
   Size:        364     104
 
BstYI r'GATC_y

Cuts at:      0      86     468 
   Size:         86     382
 
EarI CTCTTCn'nnn_

Cuts at:      0     203     468 
   Size:        203     265
 
EcoRI G'AATT_C

Cuts at:      0     104     468 
   Size:        104     364
 
MslI CAynn'nnrTG

Cuts at:      0     419     468 
   Size:        419      49
 
MspA1I CmG'CkG

Cuts at:      0     147     468 
   Size:        147     321
 
PfoI T'CCnGG_A

Cuts at:      0      89     468 
   Size:         89     379
 
PsiI TTA'TAA

Cuts at:      0     317     468 
   Size:        317     151
 
PspXI vC'TCGA_Gb

Cuts at:      0      43     468 
   Size:         43     425
 
PvuII CAG'CTG

Cuts at:      0     147     468 
   Size:        147     321
 
SmlI C'TyrA_G

Cuts at:      0      43     468 
   Size:         43     425
 
SspI AAT'ATT

Cuts at:      0     180     313     468 
   Size:        180     133     155

  Fragments arranged by size: 

                 180     155     133
 
XhoI C'TCGA_G

Cuts at:      0      43     468 
   Size:         43     425
 
XmnI GAAnn'nnTTC

Cuts at:      0     104     468 
   Size:        104     364
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI     AvaI     BbsI    BciVI     BclI Bme1580I
    BseYI Bsp1286I    BstYI     EarI    EcoRI     MslI   MspA1I     PfoI
     PsiI    PspXI    PvuII     SmlI     SspI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     ZraI
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