Restriction map for pF1KB6760
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AviII       
AvrII       BalI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoT22I     FauI        FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HindIII     HinfI       HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          215  AAATTT               [GA]^AATT[CT]
AfaI          399  GTAC                 GT^AC
ApaI          114  GGGCCC               GGGCC^C
ApoI          215  AAATTT               [GA]^AATT[CT]
AspI          291  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          70  GGACC                G^G[AT]CC
BanI          357  GGTGCC               G^G[CT][GA]CC
BanII         114  GGGCCC               G[GA]GC[CT]^C
BfaI          409  CTAG                 C^TAG
BmyI          114  GGGCCC               G[AGT]GC[ACT]^C
BpmI          281  CTCCAG               CTGGAG(16/14)
Bsp1286I      114  GGGCCC               G[AGT]GC[ACT]^C
BsrDI         239  GCAATG               GCAATG(2/0)
BssKI         354  CCTGG                ^CC[ACGT]GG
BstAPI        245  GCAGCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        159  CATCC                GGATG(2/0)
BstNI         354  CCTGG                CC^[AT]GG
BstXI         104  CCACCATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI            4  GCGC                 GCG^C
DdeI          272  CTCAG                C^T[ACGT]AG
DraII          69  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoNI         130  CCTTACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       69  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        354  CCTGG                ^CC[AT]GG
EcoRV         259  GATATC               GAT^ATC
EcoT14I       135  CCAAGG               C^C[AT][AT]GG
FokI          159  CATCC                GGATG(9/13)
HaeII           3  GGCGCT               [GA]GCGC^[CT]
HhaI            4  GCGC                 GCG^C
HinP1I          4  GCGC                 G^CGC
HincII        288  GTTGAC               GT[CT]^[GA]AC
HindII        288  GTTGAC               GT[CT]^[GA]AC
MaeI          409  CTAG                 C^TAG
MboII         149  TCTTC                GAAGA(8/7)
MseI           28  TTAA                 T^TAA
MslI          105  CACCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          354  CCTGG                CC^[AT]GG
NlaIII        111  CATG                 CATG^
PflFI         291  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          69  AGGACCT              [GA]G^G[AT]CC[CT]
PstI           65  CTGCAG               CTGCA^G
RsaI          399  GTAC                 GT^AC
ScrFI         354  CCTGG                CC^[ACGT]GG
SfaNI         248  GCATC                GCATC(5/9)
StyI          135  CCAAGG               C^C[AT][AT]GG
Tru9I          28  TTAA                 T^TAA
Tth111I       291  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI           12  GCGG                 CCGC(-3/-1)
              267  GCGG                
AlwNI          68  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              246  CAGCATCTG           
BamHI          14  GGATCC               G^GATCC
               45  GGATCC              
BbvI          245  GCAGC                GCAGC(8/12)
              324  GCTGC               
BsaI          269  GGTCTC               GGTCTC(1/5)
              278  GGTCTC              
BsaJI         135  CCAAGG               C^C[ACGT][ACGT]GG
              353  CCCTGG              
BsiYI          93  CCACTGGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              130  CCTTACCAAGG         
BslI           93  CCACTGGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              130  CCTTACCAAGG         
BsmAI         270  GTCTC                GTCTC(1/5)
              279  GTCTC               
BsrI           95  ACTGG                ACTGG(1/-1)
               57  CCAGT               
BstYI          14  GGATCC               [GA]^GATC[CT]
               45  GGATCC              
Cac8I          99  GCAGGC               GC[ACGT]^[ACGT]GC
              320  GCTCGC              
DpnII          15  GATC                 ^GATC
               46  GATC                
Fnu4HI        245  GCAGC                GC^[ACGT]GC
              324  GCTGC               
HphI           79  GGTGA                GGTGA(8/7)
              222  TCACC               
ItaI          245  GCAGC                GC^[ACGT]GC
              324  GCTGC               
MboI           15  GATC                 ^GATC
               46  GATC                
MflI           14  GGATCC               [GA]^GATC[CT]
               45  GGATCC              
MwoI          239  GCAATGGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              245  GCAGCATCTGC         
NdeII          15  GATC                 ^GATC
               46  GATC                
Sau3AI         15  GATC                 ^GATC
               46  GATC                
SfcI           65  CTGCAG               C^T[GA][CT]AG
              170  CTACAG              
TseI          245  GCAGC                G^C[AT]GC
              324  GCTGC               
Tsp509I        26  AATT                 ^AATT
              216  AATT                
XhoII          14  GGATCC               [GA]^GATC[CT]
               45  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AlwI        Cfr13I      HaeIII      MnlI        NlaIV       
Sau96I      TspRI       
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