Restriction map for pF1KB6343
 (Circular) (Six-base) MAPSORT of: pF1KB6343.seq  Check: 4569  from: 1  to: 3905



pF1KB6343  3905 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:    943     943 
   Size:       3905
 
Acc65I G'GTAC_C

Cuts at:    921     921 
   Size:       3905
 
AclI AA'CG_TT

Cuts at:   3745    3745 
   Size:       3905
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    520     647    1730    2162    2909    3177     520 
   Size:        127    1083     432     747     268    1248

  Fragments arranged by size: 

                1248    1083     747     432     268     127
 
AflIII A'CryG_T

Cuts at:   1257    2361    3338    1257 
   Size:       1104     977    1824

  Fragments arranged by size: 

                1824    1104     977
 
AgeI A'CCGG_T

Cuts at:   2293    2293 
   Size:       3905
 
AlwNI CAG_nnn'CTG

Cuts at:   1210    2777    1210 
   Size:       1567    2338
 
ApaLI G'TGCA_C

Cuts at:   2675    2675 
   Size:       3905
 
ApoI r'AATT_y

Cuts at:    321     506     520     715     909    3258    3514     321 
   Size:        185      14     195     194    2349     256     712

  Fragments arranged by size: 

                2349     712     256     195     194     185      14
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3905
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3905
 
AvaI C'yCGr_G

Cuts at:    925     925 
   Size:       3905
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    863     896     863 
   Size:         33    3872
 
BamHI G'GATC_C

Cuts at:    930     930 
   Size:       3905
 
BanI G'GyrC_C

Cuts at:    268     921    1335    1583    1618     268 
   Size:        653     414     248      35    2555

  Fragments arranged by size: 

                2555     653     414     248      35
 
BanII G_rGCy'C

Cuts at:    919    1949     919 
   Size:       1030    2875
 
BbeI G_GCGC'C

Cuts at:   1339    1587    1339 
   Size:        248    3657
 
BbsI GAAGACnn'nnnn_

Cuts at:    899     899 
   Size:       3905
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3871
 
BciVI GTATCCnnnnn_n'

Cuts at:   1803    2564    3905    1803 
   Size:        761    1341    1803

  Fragments arranged by size: 

                1803    1341     761
 
BclI T'GATC_A

Cuts at:   1425    1425 
   Size:       3905
 
BglII A'GATC_T

Cuts at:    298    1420    3457     298 
   Size:       1122    2037     746

  Fragments arranged by size: 

                2037    1122     746
 
BlpI GC'TnA_GC

Cuts at:   1017    1017 
   Size:       3905
 
Bme1580I G_kGCm'C

Cuts at:   1530    1623    2679    1530 
   Size:         93    1056    2756

  Fragments arranged by size: 

                2756    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1117    1198    1533    3677    1117 
   Size:         81     335    2144    1345

  Fragments arranged by size: 

                2144    1345     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2272    2272 
   Size:       3905
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    464     464 
   Size:       3905
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1081    1960    2452    2750    1081 
   Size:        879     492     298    2236

  Fragments arranged by size: 

                2236     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3609    3609 
   Size:       3905
 
BsaAI yAC'GTr

Cuts at:   1258    1258 
   Size:       3905
 
BsaBI GATnn'nnATC

Cuts at:      6     825    1419    3456       6 
   Size:        819     594    2037     455

  Fragments arranged by size: 

                2037     819     594     455
 
BsaHI Gr'CG_yC

Cuts at:   1336    1584    2286    3782    1336 
   Size:        248     702    1496    1459

  Fragments arranged by size: 

                1496    1459     702     248
 
BsaWI w'CCGG_w

Cuts at:    585    1094    1615    2293    2567    2714    3125     585 
   Size:        509     521     678     274     147     411    1365

  Fragments arranged by size: 

                1365     678     521     509     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    419     419 
   Size:       3905
 
BseYI C'CCAG_C

Cuts at:   1330    2088    2665    3161    1330 
   Size:        758     577     496    2074

  Fragments arranged by size: 

                2074     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1447    1493    2701     104 
   Size:       1343      46    1208    1308

  Fragments arranged by size: 

                1343    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    345     919    1697    1887    2679     345 
   Size:        574     778     190     792    1571

  Fragments arranged by size: 

                1571     792     778     574     190
 
Bsp1286I G_dGCh'C

Cuts at:    345     919    1530    1623    1697    1887    1949    2679     345 
   Size:        574     611      93      74     190      62     730    1571

  Fragments arranged by size: 

                1571     730     611     574     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:    585    3125     585 
   Size:       2540    1365
 
BspHI T'CATG_A

Cuts at:    161    3081     161 
   Size:       2920     985
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    955    1169    1471    1852     955 
   Size:        214     302     381    3008

  Fragments arranged by size: 

                3008     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2197    2251    3735    3900      50 
   Size:       2147      54    1484     165      55

  Fragments arranged by size: 

                2147    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    473     585    1817     473 
   Size:        112    1232    2561

  Fragments arranged by size: 

                2561    1232     112
 
BsrFI r'CCGG_y

Cuts at:   1903    2084    2293    1903 
   Size:        181     209    3515

  Fragments arranged by size: 

                3515     209     181
 
BssSI C'ACGA_G

Cuts at:   2176    2534    2176 
   Size:        358    3547
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1282    1282 
   Size:       3905
 
BstBI TT'CG_AA

Cuts at:   2266    2266 
   Size:       3905
 
BstYI r'GATC_y

Cuts at:      1     298     444     753     930    1397    1420    1755    2001 
   Size:        297     146     309     177     467      23     335     246

Cuts at:   2001    3013    3099    3111    3457       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     309     297     246
                 177     146      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    815     815 
   Size:       3905
 
BtgI C'CryG_G

Cuts at:    174     264     174 
   Size:         90    3815
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1855    2036    2199    1855 
   Size:        181     163    3561

  Fragments arranged by size: 

                3561     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1148    1148 
   Size:       3905
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    663     698     663 
   Size:         35    3870
 
DraI TTT'AAA

Cuts at:    720     904     720 
   Size:        184    3721
 
DrdI GACnn_nn'nnGTC

Cuts at:   1611    2469    1611 
   Size:        858    3047
 
EaeI y'GGCC_r

Cuts at:   1490    1664    2055    2082    3427    1490 
   Size:        174     391      27    1345    1968

  Fragments arranged by size: 

                1968    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1490    1490 
   Size:       3905
 
EarI CTCTTCn'nnn_

Cuts at:   1928    2138    1928 
   Size:        210    3695
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2113    2423    2569    3715    2113 
   Size:        310     146    1146    2303

  Fragments arranged by size: 

                2303    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    917     917 
   Size:       3905
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    464     520     647    1730    2162    2909    3177     464 
   Size:         56     127    1083     432     747     268    1192

  Fragments arranged by size: 

                1192    1083     747     432     268     127      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:    804     804 
   Size:       3905
 
EcoO109I rG'GnC_Cy

Cuts at:   1044    1044 
   Size:       3905
 
EcoRI G'AATT_C

Cuts at:    909     909 
   Size:       3905
 
EcoRV GAT'ATC

Cuts at:    827     827 
   Size:       3905
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1924    1924 
   Size:       3905
 
FspI TGC'GCA

Cuts at:   1686    1686 
   Size:       3905
 
HaeII r_GCGC'y

Cuts at:   1339    1587    2609    3588    1339 
   Size:        248    1022     979    1656

  Fragments arranged by size: 

                1656    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1747    1777    1779    1809    1747 
   Size:         30       2      30    3843

  Fragments arranged by size: 

                3843      30      30       2
 
HincII GTy'rAC

Cuts at:    944     944 
   Size:       3905
 
KasI G'GCGC_C

Cuts at:   1335    1583    1335 
   Size:        248    3657
 
KpnI G_GTAC'C

Cuts at:    925     925 
   Size:       3905
 
MluI A'CGCG_T

Cuts at:   3338    3338 
   Size:       3905
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    218    2576    2760     218 
   Size:       2358     184    1363

  Fragments arranged by size: 

                2358    1363     184
 
MscI TGG'CCA

Cuts at:   1666    1666 
   Size:       3905
 
MslI CAynn'nnrTG

Cuts at:    179    1894    2021     179 
   Size:       1715     127    2063

  Fragments arranged by size: 

                2063    1715     127
 
MspA1I CmG'CkG

Cuts at:    535    1016    1330    1690    2703    2948     535 
   Size:        481     314     360    1013     245    1492

  Fragments arranged by size: 

                1492    1013     481     360     314     245
 
NaeI GCC'GGC

Cuts at:   2086    2086 
   Size:       3905
 
NarI GG'CG_CC

Cuts at:   1336    1584    1336 
   Size:        248    3657
 
NcoI C'CATG_G

Cuts at:    174     174 
   Size:       3905
 
NgoMIV G'CCGG_C

Cuts at:   2084    2084 
   Size:       3905
 
NspI r_CATG'y

Cuts at:    958    1458    2365     958 
   Size:        500     907    2498

  Fragments arranged by size: 

                2498     907     500
 
PasI CC'CwG_GG

Cuts at:    560     560 
   Size:       3905
 
PciI A'CATG_T

Cuts at:   2361    2361 
   Size:       3905
 
PfoI T'CCnGG_A

Cuts at:    480     480 
   Size:       3905
 
PmeI GTTT'AAAC

Cuts at:    904     904 
   Size:       3905
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3044    3076    3044 
   Size:         32    3873
 
PstI C_TGCA'G

Cuts at:    952    1637     952 
   Size:        685    3220
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3905
 
PvuII CAG'CTG

Cuts at:    535    1330    1690     535 
   Size:        795     360    2750

  Fragments arranged by size: 

                2750     795     360
 
RsrII CG'GwC_CG

Cuts at:   2100    2100 
   Size:       3905
 
SacI G_AGCT'C

Cuts at:    919     919 
   Size:       3905
 
SalI G'TCGA_C

Cuts at:    942     942 
   Size:       3905
 
SapI GCTCTTCn'nnn_

Cuts at:   1928    2138    1928 
   Size:        210    3695
 
SbfI CC_TGCA'GG

Cuts at:    952     952 
   Size:       3905
 
ScaI AGT'ACT

Cuts at:    547    3422     547 
   Size:       2875    1030
 
SfcI C'TryA_G

Cuts at:     33     842     863     948    1633    2626    2817      33 
   Size:        809      21      85     685     993     191    1121

  Fragments arranged by size: 

                1121     993     809     685     191      85      21
 
SfoI GGC'GCC

Cuts at:   1337    1585    1337 
   Size:        248    3657
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3905
 
SmaI CCC'GGG

Cuts at:    927     927 
   Size:       3905
 
SmlI C'TyrA_G

Cuts at:   1060    1975    2467    2729    1060 
   Size:        915     492     262    2236

  Fragments arranged by size: 

                2236     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3905
 
SphI G_CATG'C

Cuts at:    958    1458     958 
   Size:        500    3405
 
SspI AAT'ATT

Cuts at:    837     837 
   Size:       3905
 
StyI C'CwwG_G

Cuts at:    174    1039    1393     174 
   Size:        865     354    2686

  Fragments arranged by size: 

                2686     865     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1488    1621    2080    2289    1488 
   Size:        133     459     209    3104

  Fragments arranged by size: 

                3104     459     209     133
 
TatI w'GTAC_w

Cuts at:    545    3420     545 
   Size:       2875    1030
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    429     461    3422    3454     429 
   Size:         32    2961      32     880

  Fragments arranged by size: 

                2961     880      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1702    1702 
   Size:       3905
 
XbaI T'CTAG_A

Cuts at:    337     936     337 
   Size:        599    3306
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1400    1400 
   Size:       3905
 
XmaI C'CCGG_G

Cuts at:    925     925 
   Size:       3905
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI     PfoI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII    BbvCI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I     ClaI   DraIII     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     XmnI     ZraI
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