Restriction map for pF1KE0896
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT22I     FseI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MfeI        MflI        MluI        MroI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau96I      SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         544  CGCG                 CG^CG
Alw44I        473  GTGCAC               G^TGCAC
AlwI          589  GATCC                GGATC(4/5)
ApaLI         473  GTGCAC               G^TGCAC
Asp700I       756  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          17  TGCGCA               TGC^GCA
BbsI          694  GTCTTC               GAAGAC(2/6)
BclI          644  TGATCA               T^GATCA
BpmI          743  CTGGAG               CTGGAG(16/14)
BpuAI         694  GTCTTC               GAAGAC(2/6)
BsaBI         642  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          809  GGTCTC               GGTCTC(1/5)
BseRI         115  GAGGAG               GAGGAG(10/8)
BsmFI         461  GTCCC                GGGAC(10/14)
BspMI         176  ACCTGC               ACCTGC(4/8)
BstEII        159  GGTCACC              G^GT[ACGT]ACC
BstPI         159  GGTCACC              G^GT[ACGT]ACC
BstUI         544  CGCG                 CG^CG
BstXI         287  CCAAAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           18  GCGC                 GCG^C
EcoO65I       159  GGTCACC              G^GT[ACGT]ACC
FauI          542  CCCGC                CCCGC(4/6)
FbaI          644  TGATCA               T^GATCA
FspI           17  TGCGCA               TGC^GCA
HhaI           18  GCGC                 GCG^C
HinP1I         18  GCGC                 G^CGC
HindIII       607  AAGCTT               A^AGCTT
MaeIII        160  GTCAC                ^GT[ACGT]AC
MamI          642  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          253  TTAA                 T^TAA
MvnI          544  CGCG                 CG^CG
NdeI          407  CATATG               CA^TATG
NlaIV         173  GGAACC               GG[ACGT]^[ACGT]CC
NspI          149  GCATGT               [GA]CATG^[CT]
SapI          798  GCTCTTC              GCTCTTC(1/4)
SexAI         155  ACCTGGT              A^CC[AT]GGT
SfcI          851  CTACAG               C^T[GA][CT]AG
SmlI          924  CTTGAG               C^T[CT][GA]AG
StuI          758  AGGCCT               AGG^CCT
Tru9I         253  TTAA                 T^TAA
Tsp45I        160  GTCAC                ^GT[GC]AC
XmnI          756  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          153  GTAC                 GT^AC
              228  GTAC                
AspHI         473  GTGCAC               G[AT]GC[AT]^C
              596  GTGCTC              
BbvI          120  GCAGC                GCAGC(8/12)
              871  GCTGC               
BfaI           94  CTAG                 C^TAG
              593  CTAG                
BsiHKAI       473  GTGCAC               G[AT]GC[AT]^C
              596  GTGCTC              
BsiYI         164  CCATGGTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              414  CCGCTATGTGG         
BslI          164  CCATGGTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              414  CCGCTATGTGG         
BsmAI         935  GAGAC                GTCTC(1/5)
              810  GTCTC               
DdeI          104  CTGAG                C^T[ACGT]AG
              618  CTCAG               
DsaI          164  CCATGG               C^C[GA][CT]GG
              653  CCATGG              
EarI          125  CTCTTC               CTCTTC(1/4)
              799  CTCTTC              
Fnu4HI        120  GCAGC                GC^[ACGT]GC
              871  GCTGC               
ItaI          120  GCAGC                GC^[ACGT]GC
              871  GCTGC               
Ksp632I       125  CTCTTC               CTCTTC(1/4)
              799  CTCTTC              
MaeI           94  CTAG                 C^TAG
              593  CTAG                
NcoI          164  CCATGG               C^CATGG
              653  CCATGG              
RsaI          153  GTAC                 GT^AC
              228  GTAC                
TseI          120  GCAGC                G^C[AT]GC
              871  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BalI        BmyI        BsaJI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
DpnII       EaeI        EcoRII      EcoT14I     FokI        
HaeIII      HinfI       HphI        MboI        MboII       
MluNI       MnlI        MscI        MslI        MvaI        
MwoI        NdeII       NlaIII      PleI        Sau3AI      
ScrFI       SfaNI       StyI        TspRI       
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