Restriction map for pF1KE0896
 (Linear) (Six-base) MAPSORT of: KIEE0896.seq  Check: 8026  from: 1  to: 990



KIEE0896.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     990 
   Size:        625     365
 
AleI CACnn'nnGTG

Cuts at:      0     166     990 
   Size:        166     824
 
ApaLI G'TGCA_C

Cuts at:      0     473     990 
   Size:        473     517
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     614     647     990 
   Size:        614      33     343

  Fragments arranged by size: 

                 614     343      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     687     990 
   Size:        687     303
 
BclI T'GATC_A

Cuts at:      0     644     990 
   Size:        644     346
 
Bme1580I G_kGCm'C

Cuts at:      0     477     561     990 
   Size:        477      84     429

  Fragments arranged by size: 

                 477     429      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764     990 
   Size:        764     226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     945     990 
   Size:        945      45
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     815     990 
   Size:        815     175
 
BsaBI GATnn'nnATC

Cuts at:      0     646     990 
   Size:        646     344
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     990 
   Size:        130     860
 
BseYI C'CCAG_C

Cuts at:      0     464     483     494     990 
   Size:        464      19      11     496

  Fragments arranged by size: 

                 496     464      19      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     477     600     990 
   Size:        477     123     390

  Fragments arranged by size: 

                 477     390     123
 
Bsp1286I G_dGCh'C

Cuts at:      0     477     561     600     990 
   Size:        477      84      39     390

  Fragments arranged by size: 

                 477     390      84      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     185     990 
   Size:        185     805
 
BstEII G'GTnAC_C

Cuts at:      0     159     990 
   Size:        159     831
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     294     990 
   Size:        294     696
 
BtgI C'CryG_G

Cuts at:      0     164     653     990 
   Size:        164     489     337

  Fragments arranged by size: 

                 489     337     164
 
EaeI y'GGCC_r

Cuts at:      0     109     191     239     296     422     990 
   Size:        109      82      48      57     126     568

  Fragments arranged by size: 

                 568     126     109      82      57      48
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     805     990 
   Size:        131     674     185

  Fragments arranged by size: 

                 674     185     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     764     990 
   Size:        625     139     226

  Fragments arranged by size: 

                 625     226     139
 
FspI TGC'GCA

Cuts at:      0      19     990 
   Size:         19     971
 
HindIII A'AGCT_T

Cuts at:      0     607     990 
   Size:        607     383
 
MscI TGG'CCA

Cuts at:      0     111     193     241     298     424     990 
   Size:        111      82      48      57     126     566

  Fragments arranged by size: 

                 566     126     111      82      57      48
 
MslI CAynn'nnrTG

Cuts at:      0     166     454     652     990 
   Size:        166     288     198     338

  Fragments arranged by size: 

                 338     288     198     166
 
NcoI C'CATG_G

Cuts at:      0     164     653     990 
   Size:        164     489     337

  Fragments arranged by size: 

                 489     337     164
 
NdeI CA'TA_TG

Cuts at:      0     408     990 
   Size:        408     582
 
NspI r_CATG'y

Cuts at:      0     153     990 
   Size:        153     837
 
PfoI T'CCnGG_A

Cuts at:      0     741     990 
   Size:        741     249
 
SapI GCTCTTCn'nnn_

Cuts at:      0     805     990 
   Size:        805     185
 
SexAI A'CCwGG_T

Cuts at:      0     155     990 
   Size:        155     835
 
SfcI C'TryA_G

Cuts at:      0     851     990 
   Size:        851     139
 
SmlI C'TyrA_G

Cuts at:      0     924     990 
   Size:        924      66
 
StuI AGG'CCT

Cuts at:      0     760     990 
   Size:        760     230
 
StyI C'CwwG_G

Cuts at:      0     144     164     653     990 
   Size:        144      20     489     337

  Fragments arranged by size: 

                 489     337     144      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     326     475     486     808     990 
   Size:        326     149      11     322     182

  Fragments arranged by size: 

                 326     322     182     149      11
 
TatI w'GTAC_w

Cuts at:      0     227     990 
   Size:        227     763
 
XmnI GAAnn'nnTTC

Cuts at:      0     760     990 
   Size:        760     230
 

 Enzymes that do cut:

     AcuI     AleI    ApaLI     BaeI     BbsI     BclI Bme1580I     BpmI
    BpuEI     BsaI    BsaBI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
   BstEII    BstXI     BtgI     EaeI     EarI  Eco57MI     FspI  HindIII
     MscI     MslI     NcoI     NdeI     NspI     PfoI     SapI    SexAI
     SfcI     SmlI     StuI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com