Restriction map for pF1KE0895
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AhdI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BseAI       BsgI        
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HpaI        KpnI        KspI        
MaeI        MaeII       MfeI        MluI        MroI        
MseI        MspA1I      MunI        MvnI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AgeI          362  ACCGGT               A^CCGGT
Alw44I        564  GTGCAC               G^TGCAC
ApaLI         564  GTGCAC               G^TGCAC
AspHI         564  GTGCAC               G[AT]GC[AT]^C
AspI          331  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         896  GGGCTC               G[GA]GC[CT]^C
BbeI          436  GGCGCC               GGCGC^C
BcgI          556  CGACTTTCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          260  CCGGG                CC^[GC]GG
BsaBI         588  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          884  GAGACC               GGTCTC(1/5)
BsaWI         362  ACCGGT               [AT]^CCGG[AT]
BseRI         786  CTCCTC               GAGGAG(10/8)
BsiHKAI       564  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      830  TGTACA               T^GTACA
BsrFI         362  ACCGGT               [GA]^CCGG[CT]
BsrGI         830  TGTACA               T^GTACA
BsrI           20  CCAGT                ACTGG(1/-1)
BstXI         312  CCATTTCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         587  GGATCT               [GA]^GATC[CT]
CfoI          437  GCGC                 GCG^C
Cfr10I        362  ACCGGT               [GA]^CCGG[CT]
DraII         680  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI          748  CTCTTC               CTCTTC(1/4)
EcoO109I      680  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HaeII         436  GGCGCC               [GA]GCGC^[CT]
HhaI          437  GCGC                 GCG^C
HinP1I        437  GCGC                 G^CGC
HincII        913  GTCAAC               GT[CT]^[GA]AC
HindII        913  GTCAAC               GT[CT]^[GA]AC
HindIII       815  AAGCTT               A^AGCTT
KasI          436  GGCGCC               G^GCGCC
Ksp632I       748  CTCTTC               CTCTTC(1/4)
MaeIII        841  GTCAC                ^GT[ACGT]AC
MamI          588  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          587  GGATCT               [GA]^GATC[CT]
NarI          436  GGCGCC               GG^CGCC
NciI          260  CCGGG                CC^[GC]GG
NspI           98  GCATGT               [GA]CATG^[CT]
PflFI         331  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         362  ACCGGT               A^CCGGT
PpuMI         680  AGGTCCC              [GA]G^G[AT]CC[CT]
SexAI         104  ACCTGGT              A^CC[AT]GGT
SfoI          436  GGCGCC               GGC^GCC
SmlI          873  CTTGAG               C^T[CT][GA]AG
SspBI         830  TGTACA               T^GTACA
TfiI          855  GAATC                G^A[AT]TC
Tsp45I        841  GTCAC                ^GT[GC]AC
Tsp509I        32  AATT                 ^AATT
Tth111I       331  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          191  CCAACCTGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         587  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          436  GGCGCC               G[GA]^CG[CT]C
              769  GGCGTC              
AlwI          294  GATCC                GGATC(4/5)
              587  GGATC               
Asp700I       705  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              855  GAATCCCTTC          
BanI          436  GGCGCC               G^G[CT][GA]CC
              519  GGTGCC              
BglI          346  GCCATGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              439  GCCCTCTGGGC         
BmyI          564  GTGCAC               G[AGT]GC[ACT]^C
              896  GGGCTC              
BsaHI         436  GGCGCC               G[GA]^CG[CT]C
              769  GGCGTC              
BsmBI         493  CGTCTC               CGTCTC(1/5)
               70  GAGACG              
Bsp1286I      564  GTGCAC               G[AGT]GC[ACT]^C
              896  GGGCTC              
BspHI         404  TCATGA               T^CATGA
              674  TCATGA              
BspMI         125  ACCTGC               ACCTGC(4/8)
              718  ACCTGC              
HapII         260  CCGG                 C^CGG
              363  CCGG                
Hin1I         436  GGCGCC               G[GA]^CG[CT]C
              769  GGCGTC              
HpaII         260  CCGG                 C^CGG
              363  CCGG                
MspI          260  CCGG                 C^CGG
              363  CCGG                
PleI           47  GACTC                GAGTC(4/5)
              154  GACTC               
PstI          689  CTGCAG               CTGCA^G
              720  CTGCAG              
RcaI          404  TCATGA               T^CATGA
              674  TCATGA              
TspRI          21  CAGTG                CA[GC]TG[ACGT][ACGT]^
              801  CACTG               
XmnI          705  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              855  GAATCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BalI        
BbvI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HgaI        HinfI       
HphI        ItaI        MboI        MboII       MluNI       
MnlI        MscI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
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