Restriction map for pF1KE0895
 (Linear) (Six-base) MAPSORT of: KIEE0895.seq  Check: 636  from: 1  to: 939



KIEE0895.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     727     939 
   Size:        727     212
 
AgeI A'CCGG_T

Cuts at:      0     362     939 
   Size:        362     577
 
AleI CACnn'nnGTG

Cuts at:      0     577     802     939 
   Size:        577     225     137

  Fragments arranged by size: 

                 577     225     137
 
ApaLI G'TGCA_C

Cuts at:      0     564     939 
   Size:        564     375
 
BanI G'GyrC_C

Cuts at:      0     436     519     939 
   Size:        436      83     420

  Fragments arranged by size: 

                 436     420      83
 
BanII G_rGCy'C

Cuts at:      0     900     939 
   Size:        900      39
 
BbeI G_GCGC'C

Cuts at:      0     440     939 
   Size:        440     499
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     545     579     939 
   Size:        545      34     360

  Fragments arranged by size: 

                 545     360      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     352     445     939 
   Size:        352      93     494

  Fragments arranged by size: 

                 494     352      93
 
Bme1580I G_kGCm'C

Cuts at:      0     568     939 
   Size:        568     371
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894     939 
   Size:        894      45
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     878     939 
   Size:        878      61
 
BsaBI GATnn'nnATC

Cuts at:      0     592     939 
   Size:        592     347
 
BsaHI Gr'CG_yC

Cuts at:      0     437     770     939 
   Size:        437     333     169

  Fragments arranged by size: 

                 437     333     169
 
BsaWI w'CCGG_w

Cuts at:      0     362     939 
   Size:        362     577
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     777     939 
   Size:        777     162
 
BsiHKAI G_wGCw'C

Cuts at:      0     568     939 
   Size:        568     371
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      64     499     939 
   Size:         64     435     440

  Fragments arranged by size: 

                 440     435      64
 
Bsp1286I G_dGCh'C

Cuts at:      0     568     900     939 
   Size:        568     332      39

  Fragments arranged by size: 

                 568     332      39
 
BspHI T'CATG_A

Cuts at:      0     404     674     939 
   Size:        404     270     265

  Fragments arranged by size: 

                 404     270     265
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     727     939 
   Size:        134     593     212

  Fragments arranged by size: 

                 593     212     134
 
BsrFI r'CCGG_y

Cuts at:      0     362     939 
   Size:        362     577
 
BsrGI T'GTAC_A

Cuts at:      0     830     939 
   Size:        830     109
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     319     939 
   Size:        319     620
 
BstYI r'GATC_y

Cuts at:      0     587     939 
   Size:        587     352
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     612     647     939 
   Size:        612      35     292

  Fragments arranged by size: 

                 612     292      35
 
EaeI y'GGCC_r

Cuts at:      0     140     188     218     371     668     939 
   Size:        140      48      30     153     297     271

  Fragments arranged by size: 

                 297     271     153     140      48      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     754     939 
   Size:        754     185
 
EcoO109I rG'GnC_Cy

Cuts at:      0     681     939 
   Size:        681     258
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     726     939 
   Size:        726     213
 
HaeII r_GCGC'y

Cuts at:      0     440     939 
   Size:        440     499
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     477     509     939 
   Size:        477      32     430

  Fragments arranged by size: 

                 477     430      32
 
HincII GTy'rAC

Cuts at:      0     915     939 
   Size:        915      24
 
HindIII A'AGCT_T

Cuts at:      0     815     939 
   Size:        815     124
 
KasI G'GCGC_C

Cuts at:      0     436     939 
   Size:        436     503
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     939 
   Size:        579     360
 
MscI TGG'CCA

Cuts at:      0     142     190     220     373     670     939 
   Size:        142      48      30     153     297     269

  Fragments arranged by size: 

                 297     269     153     142      48      30
 
MslI CAynn'nnrTG

Cuts at:      0     115     172     577     664     802     847     939 
   Size:        115      57     405      87     138      45      92

  Fragments arranged by size: 

                 405     138     115      92      87      57      45
 
NarI GG'CG_CC

Cuts at:      0     437     939 
   Size:        437     502
 
NspI r_CATG'y

Cuts at:      0     102     939 
   Size:        102     837
 
PpuMI rG'GwC_Cy

Cuts at:      0     681     939 
   Size:        681     258
 
PstI C_TGCA'G

Cuts at:      0     693     724     939 
   Size:        693      31     215

  Fragments arranged by size: 

                 693     215      31
 
SexAI A'CCwGG_T

Cuts at:      0     104     939 
   Size:        104     835
 
SfcI C'TryA_G

Cuts at:      0     689     720     867     939 
   Size:        689      31     147      72

  Fragments arranged by size: 

                 689     147      72      31
 
SfoI GGC'GCC

Cuts at:      0     438     939 
   Size:        438     501
 
SmlI C'TyrA_G

Cuts at:      0     873     939 
   Size:        873      66
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     780     939 
   Size:        780     159
 
TatI w'GTAC_w

Cuts at:      0     176     302     830     939 
   Size:        176     126     528     109

  Fragments arranged by size: 

                 528     176     126     109
 
Tth111I GACn'n_nGTC

Cuts at:      0     334     939 
   Size:        334     605
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     939 
   Size:        198     741
 
XmnI GAAnn'nnTTC

Cuts at:      0     709     859     939 
   Size:        709     150      80

  Fragments arranged by size: 

                 709     150      80
 

 Enzymes that do cut:

     AarI     AgeI     AleI    ApaLI     BanI    BanII     BbeI     BcgI
     BglI Bme1580I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BseRI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI    BsrGI    BstXI
    BstYI    CspCI     EaeI     EarI EcoO109I     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI     MslI     NarI     NspI
    PpuMI     PstI    SexAI     SfcI     SfoI     SmlI    TaqII     TatI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BsaAI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI     XbaI     XhoI     XmaI
     ZraI
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