Restriction map for pF1KE0890
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspHI       AspI        AvaI        AviII       
AvrII       BanI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT14I     FauI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AciI          390  CCGC                 CCGC(-3/-1)
AhdI          608  GACAGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          873  CCTAAGG              CC^T[ACGT]AGG
Asp700I       855  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         608  GACAGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII          45  GGACC                G^G[AT]CC
BamHI         446  GGATCC               G^GATCC
BanII         896  GAGCCC               G[GA]GC[CT]^C
BbvCI         186  CCTCAGC              CCTCAGC(-5/-2)
BbvI           66  GCAGC                GCAGC(8/12)
BmyI          896  GAGCCC               G[AGT]GC[ACT]^C
BsaJI         899  CCCTGG               C^C[ACGT][ACGT]GG
BsiEI         360  CGATCG               CG[GA][CT]^CG
Bsp1286I      896  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      830  TGTACA               T^GTACA
BspHI         404  TCATGA               T^CATGA
BspMI         125  ACCTGC               ACCTGC(4/8)
BsrDI         848  CATTGC               GCAATG(2/0)
BsrGI         830  TGTACA               T^GTACA
BstEII        666  GGTCACC              G^GT[ACGT]ACC
BstPI         666  GGTCACC              G^GT[ACGT]ACC
BstYI         446  GGATCC               [GA]^GATC[CT]
Bsu36I        873  CCTAAGG              CC^T[ACGT]AGG
Eam1105I      608  GACAGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        873  CCTAAGG              CC^T[ACGT]AGG
EcoO65I       666  GGTCACC              G^GT[ACGT]ACC
EcoT22I       636  ATGCAT               ATGCA^T
Fnu4HI         66  GCAGC                GC^[ACGT]GC
HapII         147  CCGG                 C^CGG
HindIII       707  AAGCTT               A^AGCTT
HpaII         147  CCGG                 C^CGG
ItaI           66  GCAGC                GC^[ACGT]GC
MaeII         812  ACGT                 A^CGT
MflI          446  GGATCC               [GA]^GATC[CT]
MseI          247  TTAA                 T^TAA
MspA1I        493  CAGCTG               C[AC]G^C[GT]G
MspI          147  CCGG                 C^CGG
NsiI          636  ATGCAT               ATGCA^T
PvuI          360  CGATCG               CGAT^CG
PvuII         493  CAGCTG               CAG^CTG
RcaI          404  TCATGA               T^CATGA
SpeI          429  ACTAGT               A^CTAGT
SphI          251  GCATGC               GCATG^C
SspBI         830  TGTACA               T^GTACA
Tru9I         247  TTAA                 T^TAA
TseI           66  GCAGC                G^C[AT]GC
XbaI          327  TCTAGA               T^CTAGA
XhoII         446  GGATCC               [GA]^GATC[CT]
XmnI          855  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BclI          107  TGATCA               T^GATCA
              839  TGATCA              
BfaI          328  CTAG                 C^TAG
              430  CTAG                
BpmI          798  CTCCAG               CTGGAG(16/14)
              901  CTGGAG              
BseRI          37  CTCCTC               GAGGAG(10/8)
              481  CTCCTC              
BsrI          791  CCAGT                ACTGG(1/-1)
              800  CCAGT               
Cac8I          69  GCAGGC               GC[ACGT]^[ACGT]GC
              251  GCATGC              
Cfr13I         45  GGACC                G^G[ACGT]CC
              489  GGCCC               
EarI          910  CTCTTC               CTCTTC(1/4)
                3  GAAGAG              
FbaI          107  TGATCA               T^GATCA
              839  TGATCA              
Ksp632I       910  CTCTTC               CTCTTC(1/4)
                3  GAAGAG              
MaeI          328  CTAG                 C^TAG
              430  CTAG                
MwoI           60  GCCAGAGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              141  GCTCATCCGGC         
NspI           98  GCATGT               [GA]CATG^[CT]
              251  GCATGC              
PleI          154  GACTC                GAGTC(4/5)
              908  GACTC               
Sau96I         45  GGACC                G^G[ACGT]CC
              489  GGCCC               
SfaNI         638  GCATC                GCATC(5/9)
              698  GCATC               
SmlI          553  CTCAAG               C^T[CT][GA]AG
              676  CTCAAG              
TfiI           12  GAATC                G^A[AT]TC
              855  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AfaI        AluI        AlwI        BalI        BsiYI       
BslI        BsmAI       BsmFI       BssKI       BstF5I      
BstNI       DdeI        DpnII       EaeI        EcoRII      
FokI        HaeIII      HinfI       HphI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      ScrFI       Tsp45I      Tsp509I     
TspRI       
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