Restriction map for
pF1KE0890
(Circular) (Six-base) MAPSORT of: pF1KE0890.seq Check: 6646 from: 1 to: 4052
pF1KE0890 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AhdI GACnn_n'nnGTC
Cuts at: 721 721
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 554 1077 554
Size: 523 3529
BanI G'GyrC_C
Cuts at: 1068 1482 1730 1765 1068
Size: 414 248 35 3355
Fragments arranged by size:
3355 414 248 35
BanII G_rGCy'C
Cuts at: 1008 1066 2096 1008
Size: 58 1030 2964
Fragments arranged by size:
2964 1030 58
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbvCI CC'TCA_GC
Cuts at: 295 295
Size: 4052
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 215 947 1572 215
Size: 732 625 2695
Fragments arranged by size:
2695 732 625
BfrBI ATG'CAT
Cuts at: 746 746
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 1677 1770 2826 1677
Size: 93 1056 2903
Fragments arranged by size:
2903 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 1030 891
Size: 139 3913
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 769 1028 1228 2107 2599 2897 646
Size: 123 259 200 879 492 298 1801
Fragments arranged by size:
1801 879 492 298 259 200 123
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 580 136
Size: 444 3608
BseYI C'CCAG_C
Cuts at: 225 599 1477 2235 2812 3308 225
Size: 374 878 758 577 496 969
Fragments arranged by size:
969 878 758 577 496 374
BsiEI CG_ry'CG
Cuts at: 104 471 1594 1640 2848 104
Size: 367 1123 46 1208 1308
Fragments arranged by size:
1308 1208 1123 367 46
BsiHKAI G_wGCw'C
Cuts at: 1066 1844 2034 2826 1066
Size: 778 190 792 2292
Fragments arranged by size:
2292 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1008 1066 1677 1770 1844 2034 2096 2826 1008
Size: 58 611 93 74 190 62 730 2234
Fragments arranged by size:
2234 730 611 190 93 74 62 58
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 512 3228 512
Size: 2716 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1102 1316 1618 1999 242
Size: 860 214 302 381 2295
Fragments arranged by size:
2295 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 955 1964 955
Size: 1009 3043
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BsrGI T'GTAC_A
Cuts at: 938 938
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstEII G'GTnAC_C
Cuts at: 774 774
Size: 4052
BstYI r'GATC_y
Cuts at: 1 554 1077 1544 1567 1902 2148 3160 3246
Size: 553 523 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 553 523 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 982 982
Size: 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 135 1295 135
Size: 1160 2892
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 166 479 762 1637 1811 2202 2229 3574 166
Size: 313 283 875 174 391 27 1345 644
Fragments arranged by size:
1345 875 644 391 313 283 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 106 1024 2075 2285 106
Size: 918 1051 210 1873
Fragments arranged by size:
1873 1051 918 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 1030 1877 2309 3056 3324 891
Size: 139 847 432 747 268 1619
Fragments arranged by size:
1619 847 747 432 268 139
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 825 2071 825
Size: 1246 2806
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 707 739 1894 1924 1926 1956 105
Size: 32 570 32 1155 30 2 30 2201
Fragments arranged by size:
2201 1155 570 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
HindIII A'AGCT_T
Cuts at: 815 815
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 168 481 764 1813 168
Size: 313 283 1049 2407
Fragments arranged by size:
2407 1049 313 283
MslI CAynn'nnrTG
Cuts at: 280 457 511 741 2041 2168 280
Size: 177 54 230 1300 127 2164
Fragments arranged by size:
2164 1300 230 177 127 54
MspA1I CmG'CkG
Cuts at: 603 1163 1477 1837 2850 3095 603
Size: 560 314 360 1013 245 1560
Fragments arranged by size:
1560 1013 560 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NsiI A_TGCA'T
Cuts at: 748 748
Size: 4052
NspI r_CATG'y
Cuts at: 210 363 1105 1605 2512 210
Size: 153 742 500 907 1750
Fragments arranged by size:
1750 907 742 500 153
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PfoI T'CCnGG_A
Cuts at: 149 550 149
Size: 401 3651
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PstI C_TGCA'G
Cuts at: 1099 1784 1099
Size: 685 3367
PvuI CG_AT'CG
Cuts at: 104 471 104
Size: 367 3685
PvuII CAG'CTG
Cuts at: 603 1477 1837 603
Size: 874 360 2818
Fragments arranged by size:
2818 874 360
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 1095 1780 2773 2964 33
Size: 1062 685 993 191 1121
Fragments arranged by size:
1121 1062 993 685 191
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 661 784 1043 1207 2122 2614 2876 661
Size: 123 259 164 915 492 262 1837
Fragments arranged by size:
1837 915 492 262 259 164 123
SpeI A'CTAG_T
Cuts at: 67 537 67
Size: 470 3582
SphI G_CATG'C
Cuts at: 363 1105 1605 363
Size: 742 500 2810
Fragments arranged by size:
2810 742 500
StyI C'CwwG_G
Cuts at: 1186 1540 1186
Size: 354 3698
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 938 3567 938
Size: 2629 1423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 435 1083 435
Size: 648 3404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 967 967
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI