Restriction map for pF1KE0890
 (Linear) (Six-base) MAPSORT of: KIEE0890.seq  Check: 5060  from: 1  to: 939



KIEE0890.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     613     939 
   Size:        613     326
 
BamHI G'GATC_C

Cuts at:      0     446     939 
   Size:        446     493
 
BanII G_rGCy'C

Cuts at:      0     900     939 
   Size:        900      39
 
BbvCI CC'TCA_GC

Cuts at:      0     187     939 
   Size:        187     752
 
BclI T'GATC_A

Cuts at:      0     107     839     939 
   Size:        107     732     100

  Fragments arranged by size: 

                 732     107     100
 
BfrBI ATG'CAT

Cuts at:      0     638     939 
   Size:        638     301
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     783     922     939 
   Size:        783     139      17

  Fragments arranged by size: 

                 783     139      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     939 
   Size:        187     752
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     661     939 
   Size:        538     123     278

  Fragments arranged by size: 

                 538     278     123
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     472     939 
   Size:         28     444     467

  Fragments arranged by size: 

                 467     444      28
 
BseYI C'CCAG_C

Cuts at:      0     117     491     939 
   Size:        117     374     448

  Fragments arranged by size: 

                 448     374     117
 
BsiEI CG_ry'CG

Cuts at:      0     363     939 
   Size:        363     576
 
Bsp1286I G_dGCh'C

Cuts at:      0     900     939 
   Size:        900      39
 
BspHI T'CATG_A

Cuts at:      0     404     939 
   Size:        404     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     939 
   Size:        134     805
 
BsrDI GCAATG_nn'

Cuts at:      0     847     939 
   Size:        847      92
 
BsrGI T'GTAC_A

Cuts at:      0     830     939 
   Size:        830     109
 
BstEII G'GTnAC_C

Cuts at:      0     666     939 
   Size:        666     273
 
BstYI r'GATC_y

Cuts at:      0     446     939 
   Size:        446     493
 
Bsu36I CC'TnA_GG

Cuts at:      0     874     939 
   Size:        874      65
 
BtsI GCAGTG_nn'

Cuts at:      0      27     939 
   Size:         27     912
 
EaeI y'GGCC_r

Cuts at:      0      58     371     654     939 
   Size:         58     313     283     285

  Fragments arranged by size: 

                 313     285     283      58
 
EarI CTCTTCn'nnn_

Cuts at:      0     916     939 
   Size:        916      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     783     922     939 
   Size:        783     139      17

  Fragments arranged by size: 

                 783     139      17
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     717     939 
   Size:        717     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     599     631     939 
   Size:         29     570      32     308

  Fragments arranged by size: 

                 570     308      32      29
 
HindIII A'AGCT_T

Cuts at:      0     707     939 
   Size:        707     232
 
MscI TGG'CCA

Cuts at:      0      60     373     656     939 
   Size:         60     313     283     283

  Fragments arranged by size: 

                 313     283     283      60
 
MslI CAynn'nnrTG

Cuts at:      0     172     349     403     633     939 
   Size:        172     177      54     230     306

  Fragments arranged by size: 

                 306     230     177     172      54
 
MspA1I CmG'CkG

Cuts at:      0     495     939 
   Size:        495     444
 
NsiI A_TGCA'T

Cuts at:      0     640     939 
   Size:        640     299
 
NspI r_CATG'y

Cuts at:      0     102     255     939 
   Size:        102     153     684

  Fragments arranged by size: 

                 684     153     102
 
PfoI T'CCnGG_A

Cuts at:      0      41     442     939 
   Size:         41     401     497

  Fragments arranged by size: 

                 497     401      41
 
PvuI CG_AT'CG

Cuts at:      0     363     939 
   Size:        363     576
 
PvuII CAG'CTG

Cuts at:      0     495     939 
   Size:        495     444
 
SmlI C'TyrA_G

Cuts at:      0     553     676     939 
   Size:        553     123     263

  Fragments arranged by size: 

                 553     263     123
 
SpeI A'CTAG_T

Cuts at:      0     429     939 
   Size:        429     510
 
SphI G_CATG'C

Cuts at:      0     255     939 
   Size:        255     684
 
TatI w'GTAC_w

Cuts at:      0     830     939 
   Size:        830     109
 
XbaI T'CTAG_A

Cuts at:      0     327     939 
   Size:        327     612
 
XmnI GAAnn'nnTTC

Cuts at:      0     859     939 
   Size:        859      80
 

 Enzymes that do cut:

     AhdI    BamHI    BanII    BbvCI     BclI    BfrBI     BpmI   Bpu10I
    BpuEI    BseRI    BseYI    BsiEI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI   BstEII    BstYI   Bsu36I     BtsI     EaeI     EarI  Eco57MI
     FalI    Hin4I  HindIII     MscI     MslI   MspA1I     NsiI     NspI
     PfoI     PvuI    PvuII     SmlI     SpeI     SphI     TatI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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