(Linear) (Six-base) MAPSORT of: KIEE0887.seq  Check: 577  from: 1  to: 1053



KIEE0887.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     841    1053 
   Size:        841     212
 
AccI GT'mk_AC

Cuts at:      0     405    1053 
   Size:        405     648
 
AclI AA'CG_TT

Cuts at:      0     773    1053 
   Size:        773     280
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1053 
   Size:        811     242
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     342    1053 
   Size:        342     711
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     595     627    1053 
   Size:        595      32     426

  Fragments arranged by size: 

                 595     426      32
 
ApoI r'AATT_y

Cuts at:      0    1040    1053 
   Size:       1040      13
 
BanII G_rGCy'C

Cuts at:      0     526     533    1053 
   Size:        526       7     520

  Fragments arranged by size: 

                 526     520       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1001    1053 
   Size:       1001      52
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     525     559    1053 
   Size:        525      34     494

  Fragments arranged by size: 

                 525     494      34
 
BclI T'GATC_A

Cuts at:      0     515    1053 
   Size:        515     538
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     728    1053 
   Size:        728     325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     463    1053 
   Size:        196     267     590

  Fragments arranged by size: 

                 590     267     196
 
Bpu10I CC'TnA_GC

Cuts at:      0     806    1053 
   Size:        806     247
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     730     861    1053 
   Size:        730     131     192

  Fragments arranged by size: 

                 730     192     131
 
BsaAI yAC'GTr

Cuts at:      0     483    1053 
   Size:        483     570
 
BsaBI GATnn'nnATC

Cuts at:      0     712    1053 
   Size:        712     341
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     193     232    1053 
   Size:        193      39     821

  Fragments arranged by size: 

                 821     193      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     663    1053 
   Size:        663     390
 
BsmI GAATG_Cn'

Cuts at:      0     448     573    1053 
   Size:        448     125     480

  Fragments arranged by size: 

                 480     448     125
 
Bsp1286I G_dGCh'C

Cuts at:      0     526     533    1053 
   Size:        526       7     520

  Fragments arranged by size: 

                 526     520       7
 
BspHI T'CATG_A

Cuts at:      0     518     563    1053 
   Size:        518      45     490

  Fragments arranged by size: 

                 518     490      45
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     841    1053 
   Size:        841     212
 
BsrGI T'GTAC_A

Cuts at:      0     944    1053 
   Size:        944     109
 
BssSI C'ACGA_G

Cuts at:      0     544    1053 
   Size:        544     509
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     702    1053 
   Size:        702     351
 
BstYI r'GATC_y

Cuts at:      0     371     632     794    1053 
   Size:        371     261     162     259

  Fragments arranged by size: 

                 371     261     259     162
 
BtgI C'CryG_G

Cuts at:      0     851    1053 
   Size:        851     202
 
EaeI y'GGCC_r

Cuts at:      0     485    1053 
   Size:        485     568
 
EarI CTCTTCn'nnn_

Cuts at:      0     868    1053 
   Size:        868     185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     463     811    1053 
   Size:        196     267     348     242

  Fragments arranged by size: 

                 348     267     242     196
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    1053 
   Size:        210     843
 
FspI TGC'GCA

Cuts at:      0     621    1053 
   Size:        621     432
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     363     395    1053 
   Size:         26     337      32     658

  Fragments arranged by size: 

                 658     337      32      26
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      30    1053 
   Size:         30    1023
 
MscI TGG'CCA

Cuts at:      0     487    1053 
   Size:        487     566
 
MslI CAynn'nnrTG

Cuts at:      0     742    1053 
   Size:        742     311
 
MspA1I CmG'CkG

Cuts at:      0     174    1053 
   Size:        174     879
 
NdeI CA'TA_TG

Cuts at:      0     490    1053 
   Size:        490     563
 
PasI CC'CwG_GG

Cuts at:      0     526    1053 
   Size:        526     527
 
PfoI T'CCnGG_A

Cuts at:      0     556    1053 
   Size:        556     497
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     763     795    1053 
   Size:        763      32     258

  Fragments arranged by size: 

                 763     258      32
 
PstI C_TGCA'G

Cuts at:      0     838    1053 
   Size:        838     215
 
PvuII CAG'CTG

Cuts at:      0     174    1053 
   Size:        174     879
 
SfcI C'TryA_G

Cuts at:      0     105     409     834     981    1053 
   Size:        105     304     425     147      72

  Fragments arranged by size: 

                 425     304     147     105      72
 
SspI AAT'ATT

Cuts at:      0      37    1053 
   Size:         37    1016
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      97     512    1053 
   Size:         97     415     541

  Fragments arranged by size: 

                 541     415      97
 
TatI w'GTAC_w

Cuts at:      0     215     290     944    1053 
   Size:        215      75     654     109

  Fragments arranged by size: 

                 654     215     109      75
 
XmnI GAAnn'nnTTC

Cuts at:      0     775    1053 
   Size:        775     278
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AlfI     AloI     ApoI    BanII
     BbsI     BcgI     BclI     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BseRI     BsgI     BsmI Bsp1286I    BspHI    BspMI    BsrGI
    BssSI    BstXI    BstYI     BtgI     EaeI     EarI  Eco57MI     FalI
     FspI    Hin4I     MmeI     MscI     MslI   MspA1I     NdeI     PasI
     PfoI     PpiI     PstI    PvuII     SfcI     SspI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI