Restriction map for
pF1KE0883
(Circular) (Six-base) MAPSORT of: pF1KE0883.seq Check: 8859 from: 1 to: 4055
pF1KE0883 4055 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 835 835
Size: 4055
AatII G_ACGT'C
Cuts at: 683 683
Size: 4055
AccI GT'mk_AC
Cuts at: 331 1093 331
Size: 762 3293
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 994 1880 2312 3059 3327 805
Size: 189 886 432 747 268 1533
Fragments arranged by size:
1533 886 747 432 268 189
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AhdI GACnn_n'nnGTC
Cuts at: 924 924
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaLI G'TGCA_C
Cuts at: 1029 2825 1029
Size: 1796 2259
ApoI r'AATT_y
Cuts at: 139 1059 3408 3664 139
Size: 920 2349 256 530
Fragments arranged by size:
2349 920 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 370 1071 1485 1733 1768 370
Size: 701 414 248 35 2657
Fragments arranged by size:
2657 701 414 248 35
BanII G_rGCy'C
Cuts at: 1069 2099 1069
Size: 1030 3025
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 855 889 855
Size: 34 4021
BciVI GTATCCnnnnn_n'
Cuts at: 757 1953 2714 4055 757
Size: 1196 761 1341 757
Fragments arranged by size:
1341 1196 761 757
BclI T'GATC_A
Cuts at: 698 1575 698
Size: 877 3178
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 165 1167 165
Size: 1002 3053
Bme1580I G_kGCm'C
Cuts at: 1033 1680 1773 2829 1033
Size: 647 93 1056 2259
Fragments arranged by size:
2259 1056 647 93
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 938 2422 938
Size: 1484 2571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1231 2110 2602 2900 1009
Size: 222 879 492 298 2164
Fragments arranged by size:
2164 879 492 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 650 1569 3606 6
Size: 644 919 2037 455
Fragments arranged by size:
2037 919 644 455
BsaHI Gr'CG_yC
Cuts at: 680 1486 1734 2436 3932 680
Size: 806 248 702 1496 803
Fragments arranged by size:
1496 806 803 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 404 1480 2238 2815 3311 404
Size: 1076 758 577 496 1148
Fragments arranged by size:
1148 1076 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 197 1033 1069 1847 2037 2829 197
Size: 836 36 778 190 792 1423
Fragments arranged by size:
1423 836 792 778 190 36
BsmBI CGTCTCn'nnnn_
Cuts at: 687 687
Size: 4055
Bsp1286I G_dGCh'C
Cuts at: 197 1033 1069 1680 1773 1847 2037 2099 2829
Size: 836 36 611 93 74 190 62 730
Cuts at: 2829 197
Size: 1423
Fragments arranged by size:
1423 836 730 611 190 93 74 62
36
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1105 1319 1621 2002 835
Size: 270 214 302 381 2888
Fragments arranged by size:
2888 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 1967 242
Size: 1725 2330
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstXI CCAn_nnnn'nTGG
Cuts at: 225 225
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261
Size: 1079 467 23 335 246 1012 86 12
Cuts at: 3261 3607 1
Size: 346 449
Fragments arranged by size:
1079 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 982 982
Size: 4055
BtgI C'CryG_G
Cuts at: 107 107
Size: 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 858 2005 2186 2349 858
Size: 1147 181 163 2564
Fragments arranged by size:
2564 1147 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 1640 1814 2205 2232 3577 1640
Size: 174 391 27 1345 2118
Fragments arranged by size:
2118 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 415 994 2078 2288 415
Size: 579 1084 210 2182
Fragments arranged by size:
2182 1084 579 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 994 1880 2312 3059 3327 805
Size: 189 886 432 747 268 1533
Fragments arranged by size:
1533 886 747 432 268 189
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 139 1059 139
Size: 920 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 910 942 948 980 1897 1927 1929 1959 910
Size: 32 6 32 917 30 2 30 3006
Fragments arranged by size:
3006 917 32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 315 332 929 1094 315
Size: 17 597 165 3276
Fragments arranged by size:
3276 597 165 17
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 1816 1816
Size: 4055
MslI CAynn'nnrTG
Cuts at: 2044 2171 2044
Size: 127 3928
MspA1I CmG'CkG
Cuts at: 603 833 1166 1480 1840 2853 3098 603
Size: 230 333 314 360 1013 245 1560
Fragments arranged by size:
1560 1013 360 333 314 245 230
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NdeI CA'TA_TG
Cuts at: 465 905 465
Size: 440 3615
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 237 1108 1608 2515 237
Size: 871 500 907 1777
Fragments arranged by size:
1777 907 871 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PshAI GACnn'nnGTC
Cuts at: 337 337
Size: 4055
PstI C_TGCA'G
Cuts at: 665 832 1102 1787 665
Size: 167 270 685 2933
Fragments arranged by size:
2933 685 270 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 603 833 1480 1840 603
Size: 230 647 360 2818
Fragments arranged by size:
2818 647 360 230
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 330 1092 330
Size: 762 3293
SapI GCTCTTCn'nnn_
Cuts at: 415 2078 2288 415
Size: 1663 210 2182
Fragments arranged by size:
2182 1663 210
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 335 661 828 1098 1783 2776 2967 33
Size: 302 326 167 270 685 993 191 1121
Fragments arranged by size:
1121 993 685 326 302 270 191 167
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1024 1210 2125 2617 2879 1024
Size: 186 915 492 262 2200
Fragments arranged by size:
2200 915 492 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 107 224 1189 1543 107
Size: 117 965 354 2619
Fragments arranged by size:
2619 965 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 418 1638 1771 2230 2439 418
Size: 1220 133 459 209 2034
Fragments arranged by size:
2034 1220 459 209 133
TatI w'GTAC_w
Cuts at: 284 3570 284
Size: 3286 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 259 291 3572 3604 259
Size: 32 3281 32 710
Fragments arranged by size:
3281 710 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 541 1550 541
Size: 1009 3046
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
XmnI GAAnn'nnTTC
Cuts at: 967 967
Size: 4055
ZraI GAC'GTC
Cuts at: 681 681
Size: 4055
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI