Restriction map for pF1KE0882
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AclI          529  AACGTT               AA^CGTT
AfaI          661  GTAC                 GT^AC
Alw44I        914  GTGCAC               G^TGCAC
ApaI          642  GGGCCC               GGGCC^C
ApaLI         914  GTGCAC               G^TGCAC
AspHI         914  GTGCAC               G[AT]GC[AT]^C
AvaI           57  CTCGAG               C^[CT]CG[GA]G
BamHI         742  GGATCC               G^GATCC
BanI          775  GGCACC               G^G[CT][GA]CC
BanII         642  GGGCCC               G[GA]GC[CT]^C
BfaI          439  CTAG                 C^TAG
BpmI          162  CTCCAG               CTGGAG(16/14)
BsaI          261  GGTCTC               GGTCTC(1/5)
BsaWI          33  TCCGGT               [AT]^CCGG[AT]
BseRI          82  CTCCTC               GAGGAG(10/8)
BsiHKAI       914  GTGCAC               G[AT]GC[AT]^C
BsoBI          57  CTCGAG               C^[CT]CG[GA]G
BsrDI         348  CATTGC               GCAATG(2/0)
BsrFI         560  ACCGGC               [GA]^CCGG[CT]
Bst1107I      849  GTATAC               GTA^TAC
BstXI         116  CCACCATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         742  GGATCC               [GA]^GATC[CT]
BstZ17I       849  GTATAC               GTA^TAC
Cfr10I        560  ACCGGC               [GA]^CCGG[CT]
DraII         642  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          755  CCATGG               C^C[GA][CT]GG
EarI           85  CTCTTC               CTCTTC(1/4)
EcoO109I      642  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       320  ATGCAT               ATGCA^T
FauI          290  CCCGC                CCCGC(4/6)
HincII        532  GTTAAC               GT[CT]^[GA]AC
HindII        532  GTTAAC               GT[CT]^[GA]AC
HpaI          532  GTTAAC               GTT^AAC
Ksp632I        85  CTCTTC               CTCTTC(1/4)
MaeI          439  CTAG                 C^TAG
MflI          742  GGATCC               [GA]^GATC[CT]
MseI          533  TTAA                 T^TAA
MslI          595  CATTCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          755  CCATGG               C^CATGG
NsiI          320  ATGCAT               ATGCA^T
PaeR7I         57  CTCGAG               C^TCGAG
PflMI         243  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      529  AACGTT               AA^CGTT
PstI          921  CTGCAG               CTGCA^G
PvuII         707  CAGCTG               CAG^CTG
RsaI          661  GTAC                 GT^AC
SfaNI         517  GATGC                GCATC(5/9)
SfcI          921  CTGCAG               C^T[GA][CT]AG
TaqI           58  TCGA                 T^CGA
TfiI          414  GAATC                G^A[AT]TC
Tru9I         533  TTAA                 T^TAA
Tsp509I       678  AATT                 ^AATT
TthHB8I        58  TCGA                 T^CGA
Van91I        243  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           57  CTCGAG               C^TCGAG
XhoII         742  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI         244  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              707  CAGCTGCTG           
BsmAI         262  GTCTC                GTCTC(1/5)
              730  GTCTC               
BsmFI         158  GGGAC                GGGAC(10/14)
              486  GGGAC               
BssKI          99  CCTGG                ^CC[ACGT]GG
              155  CCTGG               
BstF5I        516  GGATG                GGATG(2/0)
              903  GGATG               
BstNI          99  CCTGG                CC^[AT]GG
              155  CCTGG               
DdeI          302  CTCAG                C^T[ACGT]AG
              818  CTCAG               
DpnII         367  GATC                 ^GATC
              743  GATC                
EcoRII         99  CCTGG                ^CC[AT]GG
              155  CCTGG               
EcoT14I        48  CCTTGG               C^C[AT][AT]GG
              755  CCATGG              
FokI          516  GGATG                GGATG(9/13)
              903  GGATG               
HapII          34  CCGG                 C^CGG
              561  CCGG                
HpaII          34  CCGG                 C^CGG
              561  CCGG                
MaeII         530  ACGT                 A^CGT
              696  ACGT                
MboI          367  GATC                 ^GATC
              743  GATC                
MspA1I        291  CCGCTG               C[AC]G^C[GT]G
              707  CAGCTG              
MspI           34  CCGG                 C^CGG
              561  CCGG                
MvaI           99  CCTGG                CC^[AT]GG
              155  CCTGG               
NdeII         367  GATC                 ^GATC
              743  GATC                
PleI          160  GACTC                GAGTC(4/5)
              488  GACTC               
Sau3AI        367  GATC                 ^GATC
              743  GATC                
ScrFI          99  CCTGG                CC^[ACGT]GG
              155  CCTGG               
SmlI           57  CTCGAG               C^T[CT][GA]AG
              444  CTTGAG              
StyI           48  CCTTGG               C^C[AT][AT]GG
              755  CCATGG              
TspRI         137  CACTG                CA[GC]TG[ACGT][ACGT]^
              236  CACTG               
XcmI          473  CCATGTTGCGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              857  CCATTACACCTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AccI        AciI        AluI        AlwI        BalI        
BbvI        BmyI        BsaJI       BsgI        BsiYI       
BslI        Bsp1286I    BsrI        Cac8I       Cfr13I      
EaeI        Fnu4HI      HaeIII      HinfI       HphI        
ItaI        MaeIII      MboII       MluNI       MnlI        
MscI        MwoI        NlaIII      NlaIV       Sau96I      
TseI        Tsp45I      
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