Restriction map for pF1KE0880
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlpI        
BpmI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRV       FauI        FseI        FspI        
HaeII       HapII       HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Asp700I       900  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         697  GTGCTC               G[AT]GC[AT]^C
AvrII         600  CCTAGG               C^CTAGG
BclI          812  TGATCA               T^GATCA
BlnI          600  CCTAGG               C^CTAGG
BsaAI         768  CACGTA               [CT]AC^GT[GA]
BseRI         921  CTCCTC               GAGGAG(10/8)
BsiHKAI       697  GTGCTC               G[AT]GC[AT]^C
BsmFI         878  GGGAC                GGGAC(10/14)
Bsp1407I      824  TGTACA               T^GTACA
BspHI         308  TCATGA               T^CATGA
BspMI         125  ACCTGC               ACCTGC(4/8)
BsrDI         349  GCAATG               GCAATG(2/0)
BsrGI         824  TGTACA               T^GTACA
BstEII        225  GGTAACC              G^GT[ACGT]ACC
BstPI         225  GGTAACC              G^GT[ACGT]ACC
DsaI          412  CCACGG               C^C[GA][CT]GG
EcoO65I       225  GGTAACC              G^GT[ACGT]ACC
EcoRI          31  GAATTC               G^AATTC
EcoT22I       633  ATGCAT               ATGCA^T
FbaI          812  TGATCA               T^GATCA
HgaI          805  GACGC                GACGC(5/10)
HincII        205  GTTGAC               GT[CT]^[GA]AC
HindII        205  GTTGAC               GT[CT]^[GA]AC
HphI          447  GGTGA                GGTGA(8/7)
MaeII         769  ACGT                 A^CGT
MseI          847  TTAA                 T^TAA
MwoI          346  GCTGCAATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          356  CATATG               CA^TATG
NsiI          633  ATGCAT               ATGCA^T
RcaI          308  TCATGA               T^CATGA
SfcI          861  CTACAG               C^T[GA][CT]AG
SmlI          700  CTCAAG               C^T[CT][GA]AG
SspBI         824  TGTACA               T^GTACA
StuI          704  AGGCCT               AGG^CCT
TfiI          917  GAATC                G^A[AT]TC
Tru9I         847  TTAA                 T^TAA
XmnI          900  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI           31  GAATTC               [GA]^AATT[CT]
              849  AAATTC              
ApoI           31  GAATTC               [GA]^AATT[CT]
              849  AAATTC              
BbsI          658  GTCTTC               GAAGAC(2/6)
              670  GTCTTC              
BmyI          697  GTGCTC               G[AGT]GC[ACT]^C
              765  GGGCAC              
BpuAI         658  GTCTTC               GAAGAC(2/6)
              670  GTCTTC              
BsmAI         643  GTCTC                GTCTC(1/5)
              739  GTCTC               
Bsp1286I      697  GTGCTC               G[AGT]GC[ACT]^C
              765  GGGCAC              
Cac8I         243  GCTGGC               GC[ACGT]^[ACGT]GC
              695  GCGTGC              
DdeI          236  CTAAG                C^T[ACGT]AG
              868  CTGAG               
DpnII         361  GATC                 ^GATC
              813  GATC                
EarI           88  CTCTTC               CTCTTC(1/4)
              904  CTCTTC              
Ksp632I        88  CTCTTC               CTCTTC(1/4)
              904  CTCTTC              
MboI          361  GATC                 ^GATC
              813  GATC                
NdeII         361  GATC                 ^GATC
              813  GATC                
NlaIV         678  GGTTCC               GG[ACGT]^[ACGT]CC
              721  GGTTCC              
PflMI         252  CCATCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              513  CCAGGAAGTGG         
PleI          408  GAGTC                GAGTC(4/5)
              656  GAGTC               
Sau3AI        361  GATC                 ^GATC
              813  GATC                
TaqI          363  TCGA                 T^CGA
              654  TCGA                
Tsp45I        536  GTGAC                ^GT[GC]AC
              835  GTGAC               
TthHB8I       363  TCGA                 T^CGA
              654  TCGA                
Van91I        252  CCATCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              513  CCAGGAAGTGG         
XcmI          191  CCAACCTCTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              248  CCAACCATCTCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BbvI        
BfaI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MaeI        MaeIII      MboII       MluNI       
MnlI        MscI        MslI        MvaI        NlaIII      
RsaI        ScrFI       SfaNI       StyI        TseI        
Tsp509I     TspRI       
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