Restriction map for pF1KE0880
 (Circular) (Six-base) MAPSORT of: pF1KE0880.seq  Check: 2286  from: 1  to: 4040



pF1KE0880  4040 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:   1078    1078 
   Size:       4040
 
Acc65I G'GTAC_C

Cuts at:   1056    1056 
   Size:       4040
 
AclI AA'CG_TT

Cuts at:   3880    3880 
   Size:       4040
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    682    1865    2297    3044    3312     682 
   Size:       1183     432     747     268    1410

  Fragments arranged by size: 

                1410    1183     747     432     268
 
AflIII A'CryG_T

Cuts at:   1392    2496    3473    1392 
   Size:       1104     977    1959

  Fragments arranged by size: 

                1959    1104     977
 
AgeI A'CCGG_T

Cuts at:   2428    2428 
   Size:       4040
 
AleI CACnn'nnGTG

Cuts at:    940     940 
   Size:       4040
 
AlwNI CAG_nnn'CTG

Cuts at:   1345    2912    1345 
   Size:       1567    2473
 
ApaLI G'TGCA_C

Cuts at:   2810    2810 
   Size:       4040
 
ApoI r'AATT_y

Cuts at:    139     957    1044    3393    3649     139 
   Size:        818      87    2349     256     530

  Fragments arranged by size: 

                2349     818     530     256      87
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4040
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4040
 
AvaI C'yCGr_G

Cuts at:   1060    1060 
   Size:       4040
 
AvrII C'CTAG_G

Cuts at:    708     708 
   Size:       4040
 
BamHI G'GATC_C

Cuts at:   1065    1065 
   Size:       4040
 
BanI G'GyrC_C

Cuts at:   1056    1470    1718    1753    1056 
   Size:        414     248      35    3343

  Fragments arranged by size: 

                3343     414     248      35
 
BanII G_rGCy'C

Cuts at:   1054    2084    1054 
   Size:       1030    3010
 
BbeI G_GCGC'C

Cuts at:   1474    1722    1474 
   Size:        248    3792
 
BbsI GAAGACnn'nnnn_

Cuts at:    759     771     759 
   Size:         12    4028
 
BciVI GTATCCnnnnn_n'

Cuts at:   1938    2699    4040    1938 
   Size:        761    1341    1938

  Fragments arranged by size: 

                1938    1341     761
 
BclI T'GATC_A

Cuts at:    920    1560     920 
   Size:        640    3400
 
BfrBI ATG'CAT

Cuts at:    743     743 
   Size:       4040
 
BglII A'GATC_T

Cuts at:   1555    3592    1555 
   Size:       2037    2003
 
BlpI GC'TnA_GC

Cuts at:   1152    1152 
   Size:       4040
 
Bme1580I G_kGCm'C

Cuts at:    877    1665    1758    2814     877 
   Size:        788      93    1056    2103

  Fragments arranged by size: 

                2103    1056     788      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1252    1333    1668    3812    1252 
   Size:         81     335    2144    1480

  Fragments arranged by size: 

                2144    1480     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2407    2407 
   Size:       4040
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    793    1216    2095    2587    2885     793 
   Size:        423     879     492     298    1948

  Fragments arranged by size: 

                1948     879     492     423     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3744    3744 
   Size:       4040
 
BsaAI yAC'GTr

Cuts at:    878    1393     878 
   Size:        515    3525
 
BsaBI GATnn'nnATC

Cuts at:      6    1554    3591       6 
   Size:       1548    2037     455

  Fragments arranged by size: 

                2037    1548     455
 
BsaHI Gr'CG_yC

Cuts at:   1471    1719    2421    3917    1471 
   Size:        248     702    1496    1594

  Fragments arranged by size: 

                1594    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1229    1750    2428    2702    2849    3260    1229 
   Size:        521     678     274     147     411    2009

  Fragments arranged by size: 

                2009     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1020    1020 
   Size:       4040
 
BseYI C'CCAG_C

Cuts at:    544    1465    2223    2800    3296     544 
   Size:        921     758     577     496    1288

  Fragments arranged by size: 

                1288     921     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1582    1628    2836     104 
   Size:       1478      46    1208    1308

  Fragments arranged by size: 

                1478    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    809    1054    1832    2022    2814     809 
   Size:        245     778     190     792    2035

  Fragments arranged by size: 

                2035     792     778     245     190
 
Bsp1286I G_dGCh'C

Cuts at:    809     877    1054    1665    1758    1832    2022    2084    2814 
   Size:         68     177     611      93      74     190      62     730

Cuts at:   2814     809 
   Size:       2035

  Fragments arranged by size: 

                2035     730     611     190     177      93      74      68
                  62
 
BspEI T'CCGG_A

Cuts at:   3260    3260 
   Size:       4040
 
BspHI T'CATG_A

Cuts at:    416    3216     416 
   Size:       2800    1240
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    242    1090    1304    1606    1987     242 
   Size:        848     214     302     381    2295

  Fragments arranged by size: 

                2295     848     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2332    2386    3870    4035      50 
   Size:       2282      54    1484     165      55

  Fragments arranged by size: 

                2282    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    464    1952     464 
   Size:       1488    2552
 
BsrFI r'CCGG_y

Cuts at:   2038    2219    2428    2038 
   Size:        181     209    3650

  Fragments arranged by size: 

                3650     209     181
 
BsrGI T'GTAC_A

Cuts at:    932     932 
   Size:       4040
 
BssSI C'ACGA_G

Cuts at:   2311    2669    2311 
   Size:        358    3682
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1417    1417 
   Size:       4040
 
BstBI TT'CG_AA

Cuts at:   2401    2401 
   Size:       4040
 
BstEII G'GTnAC_C

Cuts at:    333     333 
   Size:       4040
 
BstYI r'GATC_y

Cuts at:      1    1065    1532    1555    1890    2136    3148    3234    3246 
   Size:       1064     467      23     335     246    1012      86      12

Cuts at:   3246    3592       1 
   Size:        346     449

  Fragments arranged by size: 

                1064    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    520     520 
   Size:       4040
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1990    2171    2334    1990 
   Size:        181     163    3696

  Fragments arranged by size: 

                3696     181     163
 
BtsI GCAGTG_nn'

Cuts at:    923     947    1283     923 
   Size:         24     336    3680

  Fragments arranged by size: 

                3680     336      24
 
DraI TTT'AAA

Cuts at:   1039    1039 
   Size:       4040
 
DrdI GACnn_nn'nnGTC

Cuts at:   1746    2604    1746 
   Size:        858    3182
 
EaeI y'GGCC_r

Cuts at:    353     479     629    1625    1799    2190    2217    3562     353 
   Size:        126     150     996     174     391      27    1345     831

  Fragments arranged by size: 

                1345     996     831     391     174     150     126      27
            
 
EagI C'GGCC_G

Cuts at:   1625    1625 
   Size:       4040
 
EarI CTCTTCn'nnn_

Cuts at:    202    1018    2063    2273     202 
   Size:        816    1045     210    1969

  Fragments arranged by size: 

                1969    1045     816     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    874    2248    2558    2704    3850     874 
   Size:       1374     310     146    1146    1064

  Fragments arranged by size: 

                1374    1146    1064     310     146
 
EcoICRI GAG'CTC

Cuts at:   1052    1052 
   Size:       4040
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    682    1865    2297    3044    3312     682 
   Size:       1183     432     747     268    1410

  Fragments arranged by size: 

                1410    1183     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1179    1179 
   Size:       4040
 
EcoRI G'AATT_C

Cuts at:    139    1044     139 
   Size:        905    3135
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2059    2059 
   Size:       4040
 
FspI TGC'GCA

Cuts at:   1821    1821 
   Size:       4040
 
HaeII r_GCGC'y

Cuts at:   1474    1722    2744    3723    1474 
   Size:        248    1022     979    1791

  Fragments arranged by size: 

                1791    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1882    1912    1914    1944    1882 
   Size:         30       2      30    3978

  Fragments arranged by size: 

                3978      30      30       2
 
HincII GTy'rAC

Cuts at:    315    1079     315 
   Size:        764    3276
 
KasI G'GCGC_C

Cuts at:   1470    1718    1470 
   Size:        248    3792
 
KpnI G_GTAC'C

Cuts at:   1060    1060 
   Size:       4040
 
MluI A'CGCG_T

Cuts at:   3473    3473 
   Size:       4040
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2711    2895    2711 
   Size:        184    3856
 
MscI TGG'CCA

Cuts at:    355     481     631    1801     355 
   Size:        126     150    1170    2594

  Fragments arranged by size: 

                2594    1170     150     126
 
MslI CAynn'nnrTG

Cuts at:    689     738     928     940    2029    2156     689 
   Size:         49     190      12    1089     127    2573

  Fragments arranged by size: 

                2573    1089     190     127      49      12
 
MspA1I CmG'CkG

Cuts at:   1151    1465    1825    2838    3083    1151 
   Size:        314     360    1013     245    2108

  Fragments arranged by size: 

                2108    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2221    2221 
   Size:       4040
 
NarI GG'CG_CC

Cuts at:   1471    1719    1471 
   Size:        248    3792
 
NdeI CA'TA_TG

Cuts at:    465     465 
   Size:       4040
 
NgoMIV G'CCGG_C

Cuts at:   2219    2219 
   Size:       4040
 
NsiI A_TGCA'T

Cuts at:    745     745 
   Size:       4040
 
NspI r_CATG'y

Cuts at:   1093    1593    2500    1093 
   Size:        500     907    2633

  Fragments arranged by size: 

                2633     907     500
 
PciI A'CATG_T

Cuts at:   2496    2496 
   Size:       4040
 
PflMI CCAn_nnn'nTGG

Cuts at:    366     627     366 
   Size:        261    3779
 
PmeI GTTT'AAAC

Cuts at:   1039    1039 
   Size:       4040
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3179    3211    3179 
   Size:         32    4008
 
PstI C_TGCA'G

Cuts at:   1087    1772    1087 
   Size:        685    3355
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4040
 
PvuII CAG'CTG

Cuts at:   1465    1825    1465 
   Size:        360    3680
 
RsrII CG'GwC_CG

Cuts at:   2235    2235 
   Size:       4040
 
SacI G_AGCT'C

Cuts at:   1054    1054 
   Size:       4040
 
SalI G'TCGA_C

Cuts at:   1077    1077 
   Size:       4040
 
SapI GCTCTTCn'nnn_

Cuts at:   2063    2273    2063 
   Size:        210    3830
 
SbfI CC_TGCA'GG

Cuts at:   1087    1087 
   Size:       4040
 
ScaI AGT'ACT

Cuts at:   3557    3557 
   Size:       4040
 
SfcI C'TryA_G

Cuts at:     33     969    1083    1768    2761    2952      33 
   Size:        936     114     685     993     191    1121

  Fragments arranged by size: 

                1121     993     936     685     191     114
 
SfoI GGC'GCC

Cuts at:   1472    1720    1472 
   Size:        248    3792
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4040
 
SmaI CCC'GGG

Cuts at:   1062    1062 
   Size:       4040
 
SmlI C'TyrA_G

Cuts at:    808    1195    2110    2602    2864     808 
   Size:        387     915     492     262    1984

  Fragments arranged by size: 

                1984     915     492     387     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4040
 
SphI G_CATG'C

Cuts at:   1093    1593    1093 
   Size:        500    3540
 
StuI AGG'CCT

Cuts at:    814     814 
   Size:       4040
 
StyI C'CwwG_G

Cuts at:    308     425     708     797    1174    1528     308 
   Size:        117     283      89     377     354    2820

  Fragments arranged by size: 

                2820     377     354     283     117      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    543    1623    1756    2215    2424     543 
   Size:       1080     133     459     209    2159

  Fragments arranged by size: 

                2159    1080     459     209     133
 
TatI w'GTAC_w

Cuts at:    932    3555     932 
   Size:       2623    1417
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3557    3589    3557 
   Size:         32    4008
 
Tth111I GACn'n_nGTC

Cuts at:   1837    1837 
   Size:       4040
 
XbaI T'CTAG_A

Cuts at:   1071    1071 
   Size:       4040
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    306     363    1535     306 
   Size:         57    1172    2811

  Fragments arranged by size: 

                2811    1172      57
 
XmaI C'CCGG_G

Cuts at:   1060    1060 
   Size:       4040
 
XmnI GAAnn'nnTTC

Cuts at:   1012    1012 
   Size:       4040
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NsiI
     NspI     PciI    PflMI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI     BaeI    BbvCI     BcgI     BglI    BmgBI     BmtI     BpmI
   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI   BssHII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NheI     NotI     NruI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     ZraI
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