Restriction map for pF1KE0880
 (Linear) (Six-base) MAPSORT of: KIEE0880.seq  Check: 2200  from: 1  to: 927



KIEE0880.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     927 
   Size:        574     353
 
AleI CACnn'nnGTG

Cuts at:      0     832     927 
   Size:        832      95
 
ApoI r'AATT_y

Cuts at:      0      31     849     927 
   Size:         31     818      78

  Fragments arranged by size: 

                 818      78      31
 
AvrII C'CTAG_G

Cuts at:      0     600     927 
   Size:        600     327
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     651     663     927 
   Size:        651      12     264

  Fragments arranged by size: 

                 651     264      12
 
BclI T'GATC_A

Cuts at:      0     812     927 
   Size:        812     115
 
BfrBI ATG'CAT

Cuts at:      0     635     927 
   Size:        635     292
 
Bme1580I G_kGCm'C

Cuts at:      0     769     927 
   Size:        769     158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     927 
   Size:        685     242
 
BsaAI yAC'GTr

Cuts at:      0     770     927 
   Size:        770     157
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     912     927 
   Size:        912      15
 
BseYI C'CCAG_C

Cuts at:      0     436     927 
   Size:        436     491
 
BsiHKAI G_wGCw'C

Cuts at:      0     701     927 
   Size:        701     226
 
Bsp1286I G_dGCh'C

Cuts at:      0     701     769     927 
   Size:        701      68     158

  Fragments arranged by size: 

                 701     158      68
 
BspHI T'CATG_A

Cuts at:      0     308     927 
   Size:        308     619
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     927 
   Size:        134     793
 
BsrDI GCAATG_nn'

Cuts at:      0     356     927 
   Size:        356     571
 
BsrGI T'GTAC_A

Cuts at:      0     824     927 
   Size:        824     103
 
BstEII G'GTnAC_C

Cuts at:      0     225     927 
   Size:        225     702
 
BtgI C'CryG_G

Cuts at:      0     412     927 
   Size:        412     515
 
BtsI GCAGTG_nn'

Cuts at:      0     815     839     927 
   Size:        815      24      88

  Fragments arranged by size: 

                 815      88      24
 
EaeI y'GGCC_r

Cuts at:      0     245     371     521     927 
   Size:        245     126     150     406

  Fragments arranged by size: 

                 406     245     150     126
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     910     927 
   Size:         94     816      17

  Fragments arranged by size: 

                 816      94      17
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     766     927 
   Size:        766     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     927 
   Size:        574     353
 
EcoRI G'AATT_C

Cuts at:      0      31     927 
   Size:         31     896
 
HincII GTy'rAC

Cuts at:      0     207     927 
   Size:        207     720
 
MscI TGG'CCA

Cuts at:      0     247     373     523     927 
   Size:        247     126     150     404

  Fragments arranged by size: 

                 404     247     150     126
 
MslI CAynn'nnrTG

Cuts at:      0     581     630     820     832     927 
   Size:        581      49     190      12      95

  Fragments arranged by size: 

                 581     190      95      49      12
 
NdeI CA'TA_TG

Cuts at:      0     357     927 
   Size:        357     570
 
NsiI A_TGCA'T

Cuts at:      0     637     927 
   Size:        637     290
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     258     519     927 
   Size:        258     261     408

  Fragments arranged by size: 

                 408     261     258
 
SfcI C'TryA_G

Cuts at:      0     861     927 
   Size:        861      66
 
SmlI C'TyrA_G

Cuts at:      0     700     927 
   Size:        700     227
 
StuI AGG'CCT

Cuts at:      0     706     927 
   Size:        706     221
 
StyI C'CwwG_G

Cuts at:      0     200     317     600     689     927 
   Size:        200     117     283      89     238

  Fragments arranged by size: 

                 283     238     200     117      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     435     927 
   Size:        435     492
 
TatI w'GTAC_w

Cuts at:      0     824     927 
   Size:        824     103
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     255     927 
   Size:        198      57     672

  Fragments arranged by size: 

                 672     198      57
 
XmnI GAAnn'nnTTC

Cuts at:      0     904     927 
   Size:        904      23
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    AvrII     BbsI     BclI    BfrBI Bme1580I
    BpuEI    BsaAI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI   BstEII     BtgI     BtsI     EaeI     EarI     EciI
  Eco57MI    EcoRI   HincII     MscI     MslI     NdeI     NsiI    PflMI
     SfcI     SmlI     StuI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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