Restriction map for pF1KE0874
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MflI        MluI        MroI        MspI        MvnI        
MwoI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AciI          363  CCGC                 CCGC(-3/-1)
AflIII        653  ACACGT               A^C[GA][CT]GT
AlwI          446  GGATC                GGATC(4/5)
AseI          636  ATTAAT               AT^TAAT
AsnI          636  ATTAAT               AT^TAAT
Asp700I        31  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          85  GTGCTC               G[AT]GC[AT]^C
BbvI          433  GCAGC                GCAGC(8/12)
BfaI          244  CTAG                 C^TAG
BmyI           85  GTGCTC               G[AGT]GC[ACT]^C
BsiHKAI        85  GTGCTC               G[AT]GC[AT]^C
Bsp1286I       85  GTGCTC               G[AGT]GC[ACT]^C
Bsp1407I      773  TGTACA               T^GTACA
BsrGI         773  TGTACA               T^GTACA
BsrI          558  ACTGG                ACTGG(1/-1)
BssKI         443  CCTGG                ^CC[ACGT]GG
BstEII         55  GGTCACC              G^GT[ACGT]ACC
BstNI         443  CCTGG                CC^[AT]GG
BstPI          55  GGTCACC              G^GT[ACGT]ACC
BstXI          20  CCATTCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         447  GATC                 ^GATC
DraII         439  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI           47  GACTTTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      439  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        55  GGTCACC              G^GT[ACGT]ACC
EcoRII        443  CCTGG                ^CC[AT]GG
EcoRV         392  GATATC               GAT^ATC
Fnu4HI        433  GCAGC                GC^[ACGT]GC
HindIII       707  AAGCTT               A^AGCTT
ItaI          433  GCAGC                GC^[ACGT]GC
MaeI          244  CTAG                 C^TAG
MaeII         655  ACGT                 A^CGT
MboI          447  GATC                 ^GATC
MfeI          493  CAATTG               C^AATTG
MspA1I        434  CAGCTG               C[AC]G^C[GT]G
MunI          493  CAATTG               C^AATTG
MvaI          443  CCTGG                CC^[AT]GG
NdeII         447  GATC                 ^GATC
PleI          861  GACTC                GAGTC(4/5)
PpuMI         439  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         735  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         636  ATTAAT               AT^TAAT
PvuII         434  CAGCTG               CAG^CTG
SapI          686  GCTCTTC              GCTCTTC(1/4)
Sau3AI        447  GATC                 ^GATC
ScrFI         443  CCTGG                CC^[ACGT]GG
SmlI           67  CTTGAG               C^T[CT][GA]AG
SspBI         773  TGTACA               T^GTACA
SspI          747  AATATT               AAT^ATT
TfiI          873  GAATC                G^A[AT]TC
TseI          433  GCAGC                G^C[AT]GC
Tsp45I         56  GTCAC                ^GT[GC]AC
XmnI           31  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI           31  GAATTT               [GA]^AATT[CT]
              539  AAATTC              
ApoI           31  GAATTT               [GA]^AATT[CT]
              539  AAATTC              
BalI          314  TGGCCA               TGG^CCA
              602  TGGCCA              
BsgI          290  GTGCAG               GTGCAG(16/14)
              295  GTGCAG              
BsiYI         306  CCTCGGCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              363  CCGCTATGTGG         
BslI          306  CCTCGGCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              363  CCGCTATGTGG         
BsmAI         796  GAGAC                GTCTC(1/5)
              646  GTCTC               
BspHI         404  TCATGA               T^CATGA
              551  TCATGA              
DsaI          317  CCATGG               C^C[GA][CT]GG
              428  CCATGG              
EaeI          314  TGGCCA               [CT]^GGCC[GA]
              602  TGGCCA              
EarI          687  CTCTTC               CTCTTC(1/4)
              766  CTCTTC              
EcoT14I       317  CCATGG               C^C[AT][AT]GG
              428  CCATGG              
HinfI         861  GACTC                G^A[ACGT]TC
              873  GAATC               
Ksp632I       687  CTCTTC               CTCTTC(1/4)
              766  CTCTTC              
MaeIII         56  GTCAC                ^GT[ACGT]AC
               72  GTTAC               
MluNI         314  TGGCCA               TGG^CCA
              602  TGGCCA              
MscI          314  TGGCCA               TGG^CCA
              602  TGGCCA              
NcoI          317  CCATGG               C^CATGG
              428  CCATGG              
PstI          467  CTGCAG               CTGCA^G
              504  CTGCAG              
RcaI          404  TCATGA               T^CATGA
              551  TCATGA              
StyI          317  CCATGG               C^C[AT][AT]GG
              428  CCATGG              
TspRI         293  CAGTG                CA[GC]TG[ACGT][ACGT]^
              479  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AfaI        AluI        AvaII       BsaJI       BsmFI       
BstF5I      Cfr13I      DdeI        FokI        HaeIII      
HphI        MboII       MnlI        MseI        MslI        
NlaIII      NlaIV       RsaI        Sau96I      SfaNI       
SfcI        Tru9I       Tsp509I     
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