Restriction map for pF1KE0874
 (Circular) (Six-base) MAPSORT of: pF1KE0874.seq  Check: 9677  from: 1  to: 4067



pF1KE0874  4067 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:   1105    1105 
   Size:       4067
 
Acc65I G'GTAC_C

Cuts at:   1083    1083 
   Size:       4067
 
AclI AA'CG_TT

Cuts at:   3907    3907 
   Size:       4067
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    169    1892    2324    3071    3339     169 
   Size:       1723     432     747     268     897

  Fragments arranged by size: 

                1723     897     747     432     268
 
AflIII A'CryG_T

Cuts at:    761    1419    2523    3500     761 
   Size:        658    1104     977    1328

  Fragments arranged by size: 

                1328    1104     977     658
 
AgeI A'CCGG_T

Cuts at:   2455    2455 
   Size:       4067
 
AleI CACnn'nnGTG

Cuts at:    352     352 
   Size:       4067
 
AlwNI CAG_nnn'CTG

Cuts at:   1372    2939    1372 
   Size:       1567    2500
 
ApaLI G'TGCA_C

Cuts at:   2837    2837 
   Size:       4067
 
ApoI r'AATT_y

Cuts at:    139     647    1071    3420    3676     139 
   Size:        508     424    2349     256     530

  Fragments arranged by size: 

                2349     530     508     424     256
 
AseI AT'TA_AT

Cuts at:     20     745      20 
   Size:        725    3342
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4067
 
AvaI C'yCGr_G

Cuts at:   1087    1087 
   Size:       4067
 
BamHI G'GATC_C

Cuts at:   1092    1092 
   Size:       4067
 
BanI G'GyrC_C

Cuts at:   1083    1497    1745    1780    1083 
   Size:        414     248      35    3370

  Fragments arranged by size: 

                3370     414     248      35
 
BanII G_rGCy'C

Cuts at:   1081    2111    1081 
   Size:       1030    3037
 
BbeI G_GCGC'C

Cuts at:   1501    1749    1501 
   Size:        248    3819
 
BciVI GTATCCnnnnn_n'

Cuts at:   1965    2726    4067    1965 
   Size:        761    1341    1965

  Fragments arranged by size: 

                1965    1341     761
 
BclI T'GATC_A

Cuts at:   1587    1587 
   Size:       4067
 
BglII A'GATC_T

Cuts at:   1582    3619    1582 
   Size:       2037    2030
 
BlpI GC'TnA_GC

Cuts at:   1179    1179 
   Size:       4067
 
Bme1580I G_kGCm'C

Cuts at:   1692    1785    2841    1692 
   Size:         93    1056    2918

  Fragments arranged by size: 

                2918    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1279    1360    1695    3839    1279 
   Size:         81     335    2144    1507

  Fragments arranged by size: 

                2144    1507     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2434    2434 
   Size:       4067
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    196    1243    2122    2614    2912     196 
   Size:       1047     879     492     298    1351

  Fragments arranged by size: 

                1351    1047     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3771    3771 
   Size:       4067
 
BsaAI yAC'GTr

Cuts at:   1420    1420 
   Size:       4067
 
BsaBI GATnn'nnATC

Cuts at:      6    1581    3618       6 
   Size:       1575    2037     455

  Fragments arranged by size: 

                2037    1575     455
 
BsaHI Gr'CG_yC

Cuts at:   1498    1746    2448    3944    1498 
   Size:        248     702    1496    1621

  Fragments arranged by size: 

                1621    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1256    1777    2455    2729    2876    3287    1256 
   Size:        521     678     274     147     411    2036

  Fragments arranged by size: 

                2036     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    544    1492    2250    2827    3323     544 
   Size:        948     758     577     496    1288

  Fragments arranged by size: 

                1288     948     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    419     424     419 
   Size:          5    4062
 
BsiEI CG_ry'CG

Cuts at:    104    1609    1655    2863     104 
   Size:       1505      46    1208    1308

  Fragments arranged by size: 

                1505    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    197    1081    1859    2049    2841     197 
   Size:        884     778     190     792    1423

  Fragments arranged by size: 

                1423     884     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    197    1081    1692    1785    1859    2049    2111    2841     197 
   Size:        884     611      93      74     190      62     730    1423

  Fragments arranged by size: 

                1423     884     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3287    3287 
   Size:       4067
 
BspHI T'CATG_A

Cuts at:    512     659    3243     512 
   Size:        147    2584    1336

  Fragments arranged by size: 

                2584    1336     147
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1117    1331    1633    2014    1117 
   Size:        214     302     381    3170

  Fragments arranged by size: 

                3170     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2359    2413    3897    4062      50 
   Size:       2309      54    1484     165      55

  Fragments arranged by size: 

                2309    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1979    1979 
   Size:       4067
 
BsrFI r'CCGG_y

Cuts at:   2065    2246    2455    2065 
   Size:        181     209    3677

  Fragments arranged by size: 

                3677     209     181
 
BsrGI T'GTAC_A

Cuts at:    881     881 
   Size:       4067
 
BssSI C'ACGA_G

Cuts at:   2338    2696    2338 
   Size:        358    3709
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1444    1444 
   Size:       4067
 
BstBI TT'CG_AA

Cuts at:   2428    2428 
   Size:       4067
 
BstEII G'GTnAC_C

Cuts at:    163     163 
   Size:       4067
 
BstXI CCAn_nnnn'nTGG

Cuts at:    135     135 
   Size:       4067
 
BstYI r'GATC_y

Cuts at:      1    1092    1559    1582    1917    2163    3175    3261    3273 
   Size:       1091     467      23     335     246    1012      86      12

Cuts at:   3273    3619       1 
   Size:        346     449

  Fragments arranged by size: 

                1091    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     425     536     107 
   Size:        318     111    3638

  Fragments arranged by size: 

                3638     318     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2017    2198    2361    2017 
   Size:        181     163    3723

  Fragments arranged by size: 

                3723     181     163
 
BtsI GCAGTG_nn'

Cuts at:    407    1310     407 
   Size:        903    3164
 
DraI TTT'AAA

Cuts at:   1066    1066 
   Size:       4067
 
DrdI GACnn_nn'nnGTC

Cuts at:    161    1773    2631     161 
   Size:       1612     858    1597

  Fragments arranged by size: 

                1612    1597     858
 
EaeI y'GGCC_r

Cuts at:    422     710    1652    1826    2217    2244    3589     422 
   Size:        288     942     174     391      27    1345     900

  Fragments arranged by size: 

                1345     942     900     391     288     174      27
 
EagI C'GGCC_G

Cuts at:   1652    1652 
   Size:       4067
 
EarI CTCTTCn'nnn_

Cuts at:    801     880    2090    2300     801 
   Size:         79    1210     210    2568

  Fragments arranged by size: 

                2568    1210     210      79
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2275    2585    2731    3877    2275 
   Size:        310     146    1146    2465

  Fragments arranged by size: 

                2465    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1079    1079 
   Size:       4067
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    169    1892    2324    3071    3339     169 
   Size:       1723     432     747     268     897

  Fragments arranged by size: 

                1723     897     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    548    1206     548 
   Size:        658    3409
 
EcoRI G'AATT_C

Cuts at:   1071    1071 
   Size:       4067
 
EcoRV GAT'ATC

Cuts at:    502     502 
   Size:       4067
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    833     925    2086     833 
   Size:         92    1161    2814

  Fragments arranged by size: 

                2814    1161      92
 
FspI TGC'GCA

Cuts at:   1848    1848 
   Size:       4067
 
HaeII r_GCGC'y

Cuts at:   1501    1749    2771    3750    1501 
   Size:        248    1022     979    1818

  Fragments arranged by size: 

                1818    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    491     523    1909    1939    1941    1971     491 
   Size:         32    1386      30       2      30    2587

  Fragments arranged by size: 

                2587    1386      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1106    1106 
   Size:       4067
 
HindIII A'AGCT_T

Cuts at:    815     815 
   Size:       4067
 
KasI G'GCGC_C

Cuts at:   1497    1745    1497 
   Size:        248    3819
 
KpnI G_GTAC'C

Cuts at:   1087    1087 
   Size:       4067
 
MfeI C'AATT_G

Cuts at:    601     601 
   Size:       4067
 
MluI A'CGCG_T

Cuts at:   3500    3500 
   Size:       4067
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2738    2922    2738 
   Size:        184    3883
 
MscI TGG'CCA

Cuts at:    424     712    1828     424 
   Size:        288    1116    2663

  Fragments arranged by size: 

                2663    1116     288
 
MslI CAynn'nnrTG

Cuts at:    133     280     352     511     706     883    2056    2183     133 
   Size:        147      72     159     195     177    1173     127    2017

  Fragments arranged by size: 

                2017    1173     195     177     159     147     127      72
            
 
MspA1I CmG'CkG

Cuts at:    544    1178    1492    1852    2865    3110     544 
   Size:        634     314     360    1013     245    1501

  Fragments arranged by size: 

                1501    1013     634     360     314     245
 
NaeI GCC'GGC

Cuts at:   2248    2248 
   Size:       4067
 
NarI GG'CG_CC

Cuts at:   1498    1746    1498 
   Size:        248    3819
 
NcoI C'CATG_G

Cuts at:    107     425     536     107 
   Size:        318     111    3638

  Fragments arranged by size: 

                3638     318     111
 
NgoMIV G'CCGG_C

Cuts at:   2246    2246 
   Size:       4067
 
NspI r_CATG'y

Cuts at:   1120    1620    2527    1120 
   Size:        500     907    2660

  Fragments arranged by size: 

                2660     907     500
 
PciI A'CATG_T

Cuts at:   2523    2523 
   Size:       4067
 
PflMI CCAn_nnn'nTGG

Cuts at:    113     113 
   Size:       4067
 
PfoI T'CCnGG_A

Cuts at:    550     550 
   Size:       4067
 
PmeI GTTT'AAAC

Cuts at:   1066    1066 
   Size:       4067
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3206    3238    3206 
   Size:         32    4035
 
PpuMI rG'GwC_Cy

Cuts at:    548     548 
   Size:       4067
 
PshAI GACnn'nnGTC

Cuts at:    847     847 
   Size:       4067
 
PstI C_TGCA'G

Cuts at:    579     616    1114    1799     579 
   Size:         37     498     685    2847

  Fragments arranged by size: 

                2847     685     498      37
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4067
 
PvuII CAG'CTG

Cuts at:    544    1492    1852     544 
   Size:        948     360    2759

  Fragments arranged by size: 

                2759     948     360
 
RsrII CG'GwC_CG

Cuts at:   2262    2262 
   Size:       4067
 
SacI G_AGCT'C

Cuts at:   1081    1081 
   Size:       4067
 
SalI G'TCGA_C

Cuts at:   1104    1104 
   Size:       4067
 
SapI GCTCTTCn'nnn_

Cuts at:    801    2090    2300     801 
   Size:       1289     210    2568

  Fragments arranged by size: 

                2568    1289     210
 
SbfI CC_TGCA'GG

Cuts at:   1114    1114 
   Size:       4067
 
ScaI AGT'ACT

Cuts at:   3584    3584 
   Size:       4067
 
SfcI C'TryA_G

Cuts at:     33     575     612     993    1110    1795    2788    2979      33 
   Size:        542      37     381     117     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     542     381     191     117      37
            
 
SfoI GGC'GCC

Cuts at:   1499    1747    1499 
   Size:        248    3819
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4067
 
SmaI CCC'GGG

Cuts at:   1089    1089 
   Size:       4067
 
SmlI C'TyrA_G

Cuts at:    175    1222    2137    2629    2891     175 
   Size:       1047     915     492     262    1351

  Fragments arranged by size: 

                1351    1047     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4067
 
SphI G_CATG'C

Cuts at:   1120    1620    1120 
   Size:        500    3567
 
SspI AAT'ATT

Cuts at:    857     857 
   Size:       4067
 
StyI C'CwwG_G

Cuts at:    107     425     536    1201    1555     107 
   Size:        318     111     665     354    2619

  Fragments arranged by size: 

                2619     665     354     318     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    182    1650    1783    2242    2451     182 
   Size:       1468     133     459     209    1798

  Fragments arranged by size: 

                1798    1468     459     209     133
 
TatI w'GTAC_w

Cuts at:    284     579     881    3582     284 
   Size:        295     302    2701     769

  Fragments arranged by size: 

                2701     769     302     295
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    324     356    3584    3616     324 
   Size:         32    3228      32     775

  Fragments arranged by size: 

                3228     775      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1864    1864 
   Size:       4067
 
XbaI T'CTAG_A

Cuts at:   1098    1098 
   Size:       4067
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1562    1562 
   Size:       4067
 
XmaI C'CCGG_G

Cuts at:   1087    1087 
   Size:       4067
 
XmnI GAAnn'nnTTC

Cuts at:    143     143 
   Size:       4067
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI
    BsmBI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
     FseI    FspAI     HpaI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmlI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI
     XhoI     ZraI
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