Restriction map for pF1KE0874
 (Linear) (Six-base) MAPSORT of: KIEE0874.seq  Check: 4330  from: 1  to: 954



KIEE0874.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     954 
   Size:         61     893
 
AflIII A'CryG_T

Cuts at:      0     653     954 
   Size:        653     301
 
AleI CACnn'nnGTG

Cuts at:      0     244     954 
   Size:        244     710
 
ApoI r'AATT_y

Cuts at:      0      31     539     954 
   Size:         31     508     415

  Fragments arranged by size: 

                 508     415      31
 
AseI AT'TA_AT

Cuts at:      0     637     954 
   Size:        637     317
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     954 
   Size:         88     866
 
BseYI C'CCAG_C

Cuts at:      0     436     954 
   Size:        436     518
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     316     954 
   Size:        311       5     638

  Fragments arranged by size: 

                 638     311       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      89     954 
   Size:         89     865
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     954 
   Size:         89     865
 
BspHI T'CATG_A

Cuts at:      0     404     551     954 
   Size:        404     147     403

  Fragments arranged by size: 

                 404     403     147
 
BsrGI T'GTAC_A

Cuts at:      0     773     954 
   Size:        773     181
 
BstEII G'GTnAC_C

Cuts at:      0      55     954 
   Size:         55     899
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      27     954 
   Size:         27     927
 
BtgI C'CryG_G

Cuts at:      0     317     428     954 
   Size:        317     111     526

  Fragments arranged by size: 

                 526     317     111
 
BtsI GCAGTG_nn'

Cuts at:      0     299     954 
   Size:        299     655
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      53     954 
   Size:         53     901
 
EaeI y'GGCC_r

Cuts at:      0     314     602     954 
   Size:        314     288     352

  Fragments arranged by size: 

                 352     314     288
 
EarI CTCTTCn'nnn_

Cuts at:      0     693     772     954 
   Size:        693      79     182

  Fragments arranged by size: 

                 693     182      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     954 
   Size:         61     893
 
EcoO109I rG'GnC_Cy

Cuts at:      0     440     954 
   Size:        440     514
 
EcoRV GAT'ATC

Cuts at:      0     394     954 
   Size:        394     560
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     725     817     954 
   Size:        725      92     137

  Fragments arranged by size: 

                 725     137      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415     954 
   Size:        383      32     539

  Fragments arranged by size: 

                 539     383      32
 
HindIII A'AGCT_T

Cuts at:      0     707     954 
   Size:        707     247
 
MfeI C'AATT_G

Cuts at:      0     493     954 
   Size:        493     461
 
MscI TGG'CCA

Cuts at:      0     316     604     954 
   Size:        316     288     350

  Fragments arranged by size: 

                 350     316     288
 
MslI CAynn'nnrTG

Cuts at:      0      25     172     244     403     598     775     954 
   Size:         25     147      72     159     195     177     179

  Fragments arranged by size: 

                 195     179     177     159     147      72      25
 
MspA1I CmG'CkG

Cuts at:      0     436     954 
   Size:        436     518
 
NcoI C'CATG_G

Cuts at:      0     317     428     954 
   Size:        317     111     526

  Fragments arranged by size: 

                 526     317     111
 
PfoI T'CCnGG_A

Cuts at:      0     442     954 
   Size:        442     512
 
PpuMI rG'GwC_Cy

Cuts at:      0     440     954 
   Size:        440     514
 
PshAI GACnn'nnGTC

Cuts at:      0     739     954 
   Size:        739     215
 
PstI C_TGCA'G

Cuts at:      0     471     508     954 
   Size:        471      37     446

  Fragments arranged by size: 

                 471     446      37
 
PvuII CAG'CTG

Cuts at:      0     436     954 
   Size:        436     518
 
SapI GCTCTTCn'nnn_

Cuts at:      0     693     954 
   Size:        693     261
 
SfcI C'TryA_G

Cuts at:      0     467     504     885     954 
   Size:        467      37     381      69

  Fragments arranged by size: 

                 467     381      69      37
 
SmlI C'TyrA_G

Cuts at:      0      67     954 
   Size:         67     887
 
SspI AAT'ATT

Cuts at:      0     749     954 
   Size:        749     205
 
StyI C'CwwG_G

Cuts at:      0     317     428     954 
   Size:        317     111     526

  Fragments arranged by size: 

                 526     317     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      74     954 
   Size:         74     880
 
TatI w'GTAC_w

Cuts at:      0     176     471     773     954 
   Size:        176     295     302     181

  Fragments arranged by size: 

                 302     295     181     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     216     248     954 
   Size:        216      32     706

  Fragments arranged by size: 

                 706     216      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     954 
   Size:         35     919
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AseI    BpuEI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BsrGI   BstEII    BstXI     BtgI     BtsI
     DrdI     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I
  HindIII     MfeI     MscI     MslI   MspA1I     NcoI     PfoI    PpuMI
    PshAI     PstI    PvuII     SapI     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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