Restriction map for
pF1KE0873
(Circular) (Six-base) MAPSORT of: pF1KE0873.seq Check: 8870 from: 1 to: 4079
pF1KE0873 4079 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1904 2336 3083 3351 1904
Size: 432 747 268 2632
Fragments arranged by size:
2632 747 432 268
AflIII A'CryG_T
Cuts at: 287 326 826 1431 2535 3512 287
Size: 39 500 605 1104 977 854
Fragments arranged by size:
1104 977 854 605 500 39
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AleI CACnn'nnGTG
Cuts at: 250 337 694 250
Size: 87 357 3635
Fragments arranged by size:
3635 357 87
AlwNI CAG_nnn'CTG
Cuts at: 183 264 1384 2951 183
Size: 81 1120 1567 1311
Fragments arranged by size:
1567 1311 1120 81
ApaI G_GGCC'C
Cuts at: 620 758 620
Size: 138 3941
ApaLI G'TGCA_C
Cuts at: 555 2849 555
Size: 2294 1785
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 1099 1099
Size: 4079
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 1095 1509 1757 1792 1095
Size: 414 248 35 3382
Fragments arranged by size:
3382 414 248 35
BanII G_rGCy'C
Cuts at: 116 527 620 758 1093 2123 116
Size: 411 93 138 335 1030 2072
Fragments arranged by size:
2072 1030 411 335 138 93
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BbsI GAAGACnn'nnnn_
Cuts at: 927 927
Size: 4079
BbvCI CC'TCA_GC
Cuts at: 427 427
Size: 4079
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BfrBI ATG'CAT
Cuts at: 516 516
Size: 4079
BglI GCCn_nnn'nGGC
Cuts at: 400 460 400
Size: 60 4019
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 344 559 620 758 1704 1797 2853 344
Size: 215 61 138 946 93 1056 1570
Fragments arranged by size:
1570 1056 946 215 138 93 61
BmgBI CAC'GTC
Cuts at: 329 329
Size: 4079
BmrI ACTGGGnnnn_n'
Cuts at: 760 887 961 1291 1372 1707 3851 760
Size: 127 74 330 81 335 2144 988
Fragments arranged by size:
2144 988 335 330 127 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 334 280
Size: 54 4025
Bpu10I CC'TnA_GC
Cuts at: 427 427
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1255 2134 2626 2924 1002
Size: 253 879 492 298 2157
Fragments arranged by size:
2157 879 492 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 552 3783 552
Size: 3231 848
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 1038 1068 307
Size: 30 701 30 3318
Fragments arranged by size:
3318 701 30 30
BseYI C'CCAG_C
Cuts at: 1504 2262 2839 3335 1504
Size: 758 577 496 2248
Fragments arranged by size:
2248 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4079
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 559 1093 1871 2061 2853 559
Size: 534 778 190 792 1785
Fragments arranged by size:
1785 792 778 534 190
Bsp1286I G_dGCh'C
Cuts at: 116 344 527 559 620 758 1093 1704 1797
Size: 228 183 32 61 138 335 611 93
Cuts at: 1797 1871 2061 2123 2853 116
Size: 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 335 228 190 183 138
93 74 62 61 32
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 1019 3255 1019
Size: 2236 1843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 1129 1343 1645 2026 368
Size: 761 214 302 381 2421
Fragments arranged by size:
2421 761 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4079
BsrFI r'CCGG_y
Cuts at: 2077 2258 2467 2077
Size: 181 209 3689
Fragments arranged by size:
3689 209 181
BsrGI T'GTAC_A
Cuts at: 284 284
Size: 4079
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 183 1456 183
Size: 1273 2806
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstEII G'GTnAC_C
Cuts at: 696 696
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 540 540
Size: 4079
BstYI r'GATC_y
Cuts at: 1 1104 1571 1594 1929 2175 3187 3273 3285
Size: 1103 467 23 335 246 1012 86 12
Cuts at: 3285 3631 1
Size: 346 449
Fragments arranged by size:
1103 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 248 107
Size: 141 3938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 955 1322 955
Size: 367 3712
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DraIII CAC_nnn'GTG
Cuts at: 440 440
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 479 804 1664 1838 2229 2256 3601 479
Size: 325 860 174 391 27 1345 957
Fragments arranged by size:
1345 957 860 391 325 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 334 1904 2336 3083 3351 280
Size: 54 1570 432 747 268 1008
Fragments arranged by size:
1570 1008 747 432 268 54
EcoO109I rG'GnC_Cy
Cuts at: 180 261 754 755 1218 180
Size: 81 493 1 463 3041
Fragments arranged by size:
3041 493 463 81 1
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 4079
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 434 1513 1761 2783 3762 434
Size: 1079 248 1022 979 751
Fragments arranged by size:
1079 1022 979 751 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 671 703 1921 1951 1953 1983 671
Size: 32 1218 30 2 30 2767
Fragments arranged by size:
2767 1218 32 30 30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MfeI C'AATT_G
Cuts at: 716 716
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 584 2750 2934 584
Size: 2166 184 1729
Fragments arranged by size:
2166 1729 184
MscI TGG'CCA
Cuts at: 481 1840 481
Size: 1359 2720
MslI CAynn'nnrTG
Cuts at: 247 250 337 694 883 2068 2195 247
Size: 3 87 357 189 1185 127 2131
Fragments arranged by size:
2131 1185 357 189 127 87 3
MspA1I CmG'CkG
Cuts at: 147 1190 1504 1864 2877 3122 147
Size: 1043 314 360 1013 245 1104
Fragments arranged by size:
1104 1043 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 107 107
Size: 4079
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NsiI A_TGCA'T
Cuts at: 518 518
Size: 4079
NspI r_CATG'y
Cuts at: 291 830 1132 1632 2539 291
Size: 539 302 500 907 1831
Fragments arranged by size:
1831 907 539 500 302
PciI A'CATG_T
Cuts at: 287 826 2535 287
Size: 539 1709 1831
Fragments arranged by size:
1831 1709 539
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PspOMI G'GGCC_C
Cuts at: 616 754 616
Size: 138 3941
PstI C_TGCA'G
Cuts at: 490 1126 1811 490
Size: 636 685 2758
Fragments arranged by size:
2758 685 636
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 147 1504 1864 147
Size: 1357 360 2362
Fragments arranged by size:
2362 1357 360
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SexAI A'CCwGG_T
Cuts at: 532 532
Size: 4079
SfcI C'TryA_G
Cuts at: 33 486 975 1122 1807 2800 2991 33
Size: 453 489 147 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 453 191 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 460 460
Size: 4079
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 981 1234 2149 2641 2903 981
Size: 253 915 492 262 2157
Fragments arranged by size:
2157 915 492 262 253
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StyI C'CwwG_G
Cuts at: 107 1213 1567 107
Size: 1106 354 2619
Fragments arranged by size:
2619 1106 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1662 1795 2254 2463 1662
Size: 133 459 209 3278
Fragments arranged by size:
3278 459 209 133
TatI w'GTAC_w
Cuts at: 284 1048 3594 284
Size: 764 2546 769
Fragments arranged by size:
2546 769 764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 1574 540
Size: 1034 3045
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
XmnI GAAnn'nnTTC
Cuts at: 792 792
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BcgI BmtI BseRI BsiWI BsmI BsmBI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI