Restriction map for pF1KE0873
 (Linear) (Six-base) MAPSORT of: KIEE0873.seq  Check: 3552  from: 1  to: 966



KIEE0873.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AflIII A'CryG_T

Cuts at:      0     179     218     718     966 
   Size:        179      39     500     248

  Fragments arranged by size: 

                 500     248     179      39
 
AleI CACnn'nnGTG

Cuts at:      0     142     229     586     966 
   Size:        142      87     357     380

  Fragments arranged by size: 

                 380     357     142      87
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     156     966 
   Size:         75      81     810

  Fragments arranged by size: 

                 810      81      75
 
ApaI G_GGCC'C

Cuts at:      0     512     650     966 
   Size:        512     138     316

  Fragments arranged by size: 

                 512     316     138
 
ApaLI G'TGCA_C

Cuts at:      0     447     966 
   Size:        447     519
 
BanII G_rGCy'C

Cuts at:      0       8     419     512     650     966 
   Size:          8     411      93     138     316

  Fragments arranged by size: 

                 411     316     138      93       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     819     966 
   Size:        819     147
 
BbvCI CC'TCA_GC

Cuts at:      0     319     966 
   Size:        319     647
 
BfrBI ATG'CAT

Cuts at:      0     408     966 
   Size:        408     558
 
BglI GCCn_nnn'nGGC

Cuts at:      0     292     352     966 
   Size:        292      60     614

  Fragments arranged by size: 

                 614     292      60
 
Bme1580I G_kGCm'C

Cuts at:      0     236     451     512     650     966 
   Size:        236     215      61     138     316

  Fragments arranged by size: 

                 316     236     215     138      61
 
BmgBI CAC'GTC

Cuts at:      0     221     966 
   Size:        221     745
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     652     779     853     966 
   Size:        652     127      74     113

  Fragments arranged by size: 

                 652     127     113      74
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     226     966 
   Size:        172      54     740

  Fragments arranged by size: 

                 740     172      54
 
Bpu10I CC'TnA_GC

Cuts at:      0     319     966 
   Size:        319     647
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894     966 
   Size:        894      72
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     444     966 
   Size:        444     522
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     199     229     930     960     966 
   Size:        199      30     701      30       6

  Fragments arranged by size: 

                 701     199      30      30       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     966 
   Size:        148     818
 
BsiHKAI G_wGCw'C

Cuts at:      0     451     966 
   Size:        451     515
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     236     419     451     512     650     966 
   Size:          8     228     183      32      61     138     316

  Fragments arranged by size: 

                 316     228     183     138      61      32       8
 
BspHI T'CATG_A

Cuts at:      0     911     966 
   Size:        911      55
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     260     966 
   Size:        260     706
 
BsrGI T'GTAC_A

Cuts at:      0     176     966 
   Size:        176     790
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      75     966 
   Size:         75     891
 
BstEII G'GTnAC_C

Cuts at:      0     588     966 
   Size:        588     378
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     432     966 
   Size:        432     534
 
BtgI C'CryG_G

Cuts at:      0     140     966 
   Size:        140     826
 
BtsI GCAGTG_nn'

Cuts at:      0     847     966 
   Size:        847     119
 
DraIII CAC_nnn'GTG

Cuts at:      0     332     966 
   Size:        332     634
 
EaeI y'GGCC_r

Cuts at:      0     371     696     966 
   Size:        371     325     270

  Fragments arranged by size: 

                 371     325     270
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     226     966 
   Size:        172      54     740

  Fragments arranged by size: 

                 740     172      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     153     646     647     966 
   Size:         72      81     493       1     319

  Fragments arranged by size: 

                 493     319      81      72       1
 
HaeII r_GCGC'y

Cuts at:      0     326     966 
   Size:        326     640
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     563     595     966 
   Size:        563      32     371

  Fragments arranged by size: 

                 563     371      32
 
MfeI C'AATT_G

Cuts at:      0     608     966 
   Size:        608     358
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     966 
   Size:        476     490
 
MscI TGG'CCA

Cuts at:      0     373     966 
   Size:        373     593
 
MslI CAynn'nnrTG

Cuts at:      0     139     142     229     586     775     966 
   Size:        139       3      87     357     189     191

  Fragments arranged by size: 

                 357     191     189     139      87       3
 
MspA1I CmG'CkG

Cuts at:      0      39     966 
   Size:         39     927
 
NsiI A_TGCA'T

Cuts at:      0     410     966 
   Size:        410     556
 
NspI r_CATG'y

Cuts at:      0     183     722     966 
   Size:        183     539     244

  Fragments arranged by size: 

                 539     244     183
 
PciI A'CATG_T

Cuts at:      0     179     718     966 
   Size:        179     539     248

  Fragments arranged by size: 

                 539     248     179
 
PspOMI G'GGCC_C

Cuts at:      0     508     646     966 
   Size:        508     138     320

  Fragments arranged by size: 

                 508     320     138
 
PstI C_TGCA'G

Cuts at:      0     382     966 
   Size:        382     584
 
PvuII CAG'CTG

Cuts at:      0      39     966 
   Size:         39     927
 
SexAI A'CCwGG_T

Cuts at:      0     424     966 
   Size:        424     542
 
SfcI C'TryA_G

Cuts at:      0     378     867     966 
   Size:        378     489      99

  Fragments arranged by size: 

                 489     378      99
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     352     966 
   Size:        352     614
 
SmlI C'TyrA_G

Cuts at:      0     873     966 
   Size:        873      93
 
TatI w'GTAC_w

Cuts at:      0     176     940     966 
   Size:        176     764      26

  Fragments arranged by size: 

                 764     176      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     432     966 
   Size:        432     534
 
XmnI GAAnn'nnTTC

Cuts at:      0     684     966 
   Size:        684     282
 

 Enzymes that do cut:

   AflIII     AleI    AlwNI     ApaI    ApaLI    BanII     BbsI    BbvCI
    BfrBI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaXI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrGI
   BstAPI   BstEII    BstXI     BtgI     BtsI   DraIII     EaeI  Eco57MI
 EcoO109I    HaeII    Hin4I     MfeI     MmeI     MscI     MslI   MspA1I
     NsiI     NspI     PciI   PspOMI     PstI    PvuII    SexAI     SfcI
     SfiI     SmlI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BglII     BlpI     BmtI     BplI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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