Restriction map for pF1KE0870
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# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BanI        BanII       BbeI        
BbrPI       BcgI        BclI        BcnI        BfrI        
BglII       BlnI        BsaAI       BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MfeI        MluI        
MroI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AocI          777  CCTGAGG              CC^T[ACGT]AGG
AspHI         380  GTGCTC               G[AT]GC[AT]^C
AvaI          579  CCCGAG               C^[CT]CG[GA]G
BalI          371  TGGCCA               TGG^CCA
BamHI         680  GGATCC               G^GATCC
BciVI         454  GGATAC               GTATCC(6/5)
BglI          313  GCCTCATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          552  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      552  GCTGAGC              GC^T[ACGT]AGC
BsgI          163  CTGCAC               GTGCAG(16/14)
BsiHKAI       380  GTGCTC               G[AT]GC[AT]^C
BsmFI         263  GGGAC                GGGAC(10/14)
BsoBI         579  CCCGAG               C^[CT]CG[GA]G
BspMI         211  ACCTGC               ACCTGC(4/8)
BsrDI         122  GCAATG               GCAATG(2/0)
BstF5I        231  CATCC                GGATG(2/0)
BstXI         143  CCATCAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        777  CCTGAGG              CC^T[ACGT]AGG
CelII         552  GCTGAGC              GC^T[ACGT]AGC
DsaI          350  CCATGG               C^C[GA][CT]GG
EaeI          371  TGGCCA               [CT]^GGCC[GA]
EarI          181  CTCTTC               CTCTTC(1/4)
Eco81I        777  CCTGAGG              CC^T[ACGT]AGG
EcoNI          20  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       350  CCATGG               C^C[AT][AT]GG
FokI          231  CATCC                GGATG(9/13)
HinfI         408  GAATC                G^A[ACGT]TC
HphI          641  TCACC                GGTGA(8/7)
Ksp632I       181  CTCTTC               CTCTTC(1/4)
MaeII          14  ACGT                 A^CGT
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
MseI          854  TTAA                 T^TAA
MslI          168  CACCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        427  CAGCTG               C[AC]G^C[GT]G
NcoI          350  CCATGG               C^CATGG
PstI          504  CTGCAG               CTGCA^G
PvuII         427  CAGCTG               CAG^CTG
SfaNI         227  GCATC                GCATC(5/9)
SmlI          802  CTTGAG               C^T[CT][GA]AG
SpeI          480  ACTAGT               A^CTAGT
StuI          707  AGGCCT               AGG^CCT
StyI          350  CCATGG               C^C[AT][AT]GG
TfiI          408  GAATC                G^A[AT]TC
Tru9I         854  TTAA                 T^TAA
TspRI         737  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Asp700I       705  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              906  GAATGCTTTC          
AvaII         264  GGACC                G^G[AT]CC
              615  GGTCC               
BbsI           37  GTCTTC               GAAGAC(2/6)
              127  GTCTTC              
BbvCI         588  GCTGAGG              CCTCAGC(-5/-2)
              691  GCTGAGG             
BmyI          380  GTGCTC               G[AGT]GC[ACT]^C
              422  GTGCCC              
BpmI           51  CTCCAG               CTGGAG(16/14)
               24  CTGGAG              
BpuAI          37  GTCTTC               GAAGAC(2/6)
              127  GTCTTC              
BsaBI         154  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              594  GATCTTTATC          
BsaI          208  GAGACC               GGTCTC(1/5)
              624  GGTCTC              
BsaJI          42  CCTGGG               C^C[ACGT][ACGT]GG
              350  CCATGG              
BsmAI         208  GAGAC                GTCTC(1/5)
              625  GTCTC               
BsmI          239  GAATGC               GAATGC(1/-1)
              906  GAATGC              
Bsp1286I      380  GTGCTC               G[AGT]GC[ACT]^C
              422  GTGCCC              
BstYI         593  GGATCT               [GA]^GATC[CT]
              680  GGATCC              
Cac8I         397  GCCAGC               GC[ACGT]^[ACGT]GC
              790  GCCAGC              
MamI          154  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              594  GATCTTTATC          
MflI          593  GGATCT               [GA]^GATC[CT]
              680  GGATCC              
NlaIV         263  GGGACC               GG[ACGT]^[ACGT]CC
              680  GGATCC              
SfcI          504  CTGCAG               C^T[GA][CT]AG
              836  CTGTAG              
XhoII         593  GGATCT               [GA]^GATC[CT]
              680  GGATCC              
XmnI          705  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              906  GAATGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        AlwI        BbvI        BfaI        
BsiYI       BslI        BsrI        BssKI       BstNI       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
HaeIII      ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      RsaI        Sau3AI      Sau96I      ScrFI       
TseI        Tsp45I      
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