Restriction map for pF1KE0870
 (Linear) (Six-base) MAPSORT of: KIEE0870.seq  Check: 414  from: 1  to: 939



KIEE0870.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AvaI C'yCGr_G

Cuts at:      0     579     939 
   Size:        579     360
 
BamHI G'GATC_C

Cuts at:      0     680     939 
   Size:        680     259
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30     120     939 
   Size:         30      90     819

  Fragments arranged by size: 

                 819      90      30
 
BbvCI CC'TCA_GC

Cuts at:      0     589     692     939 
   Size:        589     103     247

  Fragments arranged by size: 

                 589     247     103
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     448     939 
   Size:        448     491
 
BglI GCCn_nnn'nGGC

Cuts at:      0     319     939 
   Size:        319     620
 
BlpI GC'TnA_GC

Cuts at:      0     553     939 
   Size:        553     386
 
Bme1580I G_kGCm'C

Cuts at:      0     426     939 
   Size:        426     513
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     478     939 
   Size:        478     461
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36      45     939 
   Size:         36       9     894

  Fragments arranged by size: 

                 894      36       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     589     692     939 
   Size:        589     103     247

  Fragments arranged by size: 

                 589     247     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     823     939 
   Size:        823     116
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     202     630     939 
   Size:        202     428     309

  Fragments arranged by size: 

                 428     309     202
 
BsaBI GATnn'nnATC

Cuts at:      0     158     598     939 
   Size:        158     440     341

  Fragments arranged by size: 

                 440     341     158
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34     939 
   Size:          4      30     905

  Fragments arranged by size: 

                 905      30       4
 
BseYI C'CCAG_C

Cuts at:      0     256     425     927     939 
   Size:        256     169     502      12

  Fragments arranged by size: 

                 502     256     169      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     939 
   Size:        148     791
 
BsiHKAI G_wGCw'C

Cuts at:      0     384     939 
   Size:        384     555
 
BsmI GAATG_Cn'

Cuts at:      0     245     912     939 
   Size:        245     667      27

  Fragments arranged by size: 

                 667     245      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     384     426     939 
   Size:        384      42     513

  Fragments arranged by size: 

                 513     384      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     220     939 
   Size:        220     719
 
BsrDI GCAATG_nn'

Cuts at:      0     129     939 
   Size:        129     810
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     150     939 
   Size:        150     789
 
BstYI r'GATC_y

Cuts at:      0     593     680     939 
   Size:        593      87     259

  Fragments arranged by size: 

                 593     259      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     778     939 
   Size:        778     161
 
BtgI C'CryG_G

Cuts at:      0     350     939 
   Size:        350     589
 
EaeI y'GGCC_r

Cuts at:      0     371     939 
   Size:        371     568
 
EarI CTCTTCn'nnn_

Cuts at:      0     187     939 
   Size:        187     752
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36      45     939 
   Size:         36       9     894

  Fragments arranged by size: 

                 894      36       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      24     939 
   Size:         24     915
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     726     939 
   Size:        726     213
 
MscI TGG'CCA

Cuts at:      0     373     939 
   Size:        373     566
 
MslI CAynn'nnrTG

Cuts at:      0     172     939 
   Size:        172     767
 
MspA1I CmG'CkG

Cuts at:      0     429     939 
   Size:        429     510
 
NcoI C'CATG_G

Cuts at:      0     350     939 
   Size:        350     589
 
PfoI T'CCnGG_A

Cuts at:      0      22     939 
   Size:         22     917
 
PstI C_TGCA'G

Cuts at:      0     508     939 
   Size:        508     431
 
PvuII CAG'CTG

Cuts at:      0     429     939 
   Size:        429     510
 
SfcI C'TryA_G

Cuts at:      0     504     836     939 
   Size:        504     332     103

  Fragments arranged by size: 

                 504     332     103
 
SmlI C'TyrA_G

Cuts at:      0     802     939 
   Size:        802     137
 
SpeI A'CTAG_T

Cuts at:      0     480     939 
   Size:        480     459
 
StuI AGG'CCT

Cuts at:      0     709     939 
   Size:        709     230
 
StyI C'CwwG_G

Cuts at:      0     350     939 
   Size:        350     589
 
TatI w'GTAC_w

Cuts at:      0     325     939 
   Size:        325     614
 
XmnI GAAnn'nnTTC

Cuts at:      0     709     910     939 
   Size:        709     201      29

  Fragments arranged by size: 

                 709     201      29
 

 Enzymes that do cut:

     AvaI    BamHI     BbsI    BbvCI    BciVI     BglI     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BstXI    BstYI
   Bsu36I     BtgI     EaeI     EarI  Eco57MI    EcoNI     FalI     MscI
     MslI   MspA1I     NcoI     PfoI     PstI    PvuII     SfcI     SmlI
     SpeI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
    BanII     BbeI     BcgI     BclI    BfrBI    BglII    BmgBI     BmtI
     BplI    BsaAI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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