Restriction map for pF1KE0862
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HinfI       HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AciI          657  CCGC                 CCGC(-3/-1)
AcsI          886  GAATTC               [GA]^AATT[CT]
AflII         777  CTTAAG               C^TTAAG
AhdI          250  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          70  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          886  GAATTC               [GA]^AATT[CT]
AspEI         250  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         400  GTGCTC               G[AT]GC[AT]^C
BanI          118  GGCACC               G^G[CT][GA]CC
BanII         158  GAGCCC               G[GA]GC[CT]^C
BbsI          267  GAAGAC               GAAGAC(2/6)
BfrI          777  CTTAAG               C^TTAAG
BpuAI         267  GAAGAC               GAAGAC(2/6)
BsiHKAI       400  GTGCTC               G[AT]GC[AT]^C
BspMI         251  ACCTGC               ACCTGC(4/8)
BsrI          558  ACTGG                ACTGG(1/-1)
BstAPI         69  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        471  GGTCACC              G^GT[ACGT]ACC
BstPI         471  GGTCACC              G^GT[ACGT]ACC
Cac8I         342  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          390  GCGC                 GCG^C
Cfr13I        782  GGCCC                G^G[ACGT]CC
DpnII         361  GATC                 ^GATC
DsaI          350  CCATGG               C^C[GA][CT]GG
Eam1105I      250  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          313  CTCTTC               CTCTTC(1/4)
EcoO65I       471  GGTCACC              G^GT[ACGT]ACC
EcoRI         886  GAATTC               G^AATTC
HapII         575  CCGG                 C^CGG
HhaI          390  GCGC                 GCG^C
HinP1I        390  GCGC                 G^CGC
HincII        247  GTTGAC               GT[CT]^[GA]AC
HindII        247  GTTGAC               GT[CT]^[GA]AC
HpaII         575  CCGG                 C^CGG
Ksp632I       313  CTCTTC               CTCTTC(1/4)
MboI          361  GATC                 ^GATC
MspI          575  CCGG                 C^CGG
NcoI          350  CCATGG               C^CATGG
NdeII         361  GATC                 ^GATC
PstI           67  CTGCAG               CTGCA^G
Sau3AI        361  GATC                 ^GATC
Sau96I        782  GGCCC                G^G[ACGT]CC
SmlI          777  CTTAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          177  GTAC                 GT^AC
              232  GTAC                
BalI           59  TGGCCA               TGG^CCA
              371  TGGCCA              
BfaI          484  CTAG                 C^TAG
              643  CTAG                
BsiYI          56  CCCTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              552  CCTTAAACTGG         
BslI           56  CCCTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              552  CCTTAAACTGG         
BsmAI         463  GTCTC                GTCTC(1/5)
              646  GTCTC               
BstXI         143  CCATTGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              236  CCAAGATGCTGG        
EcoT14I       350  CCATGG               C^C[AT][AT]GG
              617  CCTTGG              
HphI           24  GGTGA                GGTGA(8/7)
              473  TCACC               
MaeI          484  CTAG                 C^TAG
              643  CTAG                
MluNI          59  TGGCCA               TGG^CCA
              371  TGGCCA              
MscI           59  TGGCCA               TGG^CCA
              371  TGGCCA              
MseI          554  TTAA                 T^TAA
              778  TTAA                
MspA1I         73  CAGCTG               C[AC]G^C[GT]G
              586  CAGCTG              
NlaIV         118  GGCACC               GG[ACGT]^[ACGT]CC
              322  GGCTCC              
PvuII          73  CAGCTG               CAG^CTG
              586  CAGCTG              
RsaI          177  GTAC                 GT^AC
              232  GTAC                
SfcI           67  CTGCAG               C^T[GA][CT]AG
              753  CTACAG              
StyI          350  CCATGG               C^C[AT][AT]GG
              617  CCTTGG              
Tru9I         554  TTAA                 T^TAA
              778  TTAA                
Tsp45I        472  GTCAC                ^GT[GC]AC
              536  GTGAC               
Tsp509I       793  AATT                 ^AATT
              887  AATT                
XcmI          140  CCACCATTGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              272  CCATTTCTTTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        BbvI        BmyI        BsaJI       BseRI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
DdeI        EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      ItaI        MaeIII      MboII       MnlI        
MslI        MvaI        MwoI        NlaIII      ScrFI       
SfaNI       TseI        TspRI       
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