Restriction map for pF1KE0862
 (Linear) (Six-base) MAPSORT of: KIEE0862.seq  Check: 4645  from: 1  to: 939



KIEE0862.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     564     939 
   Size:        564     375
 
AflII C'TTAA_G

Cuts at:      0     777     939 
   Size:        777     162
 
AhdI GACnn_n'nnGTC

Cuts at:      0     255     939 
   Size:        255     684
 
AleI CACnn'nnGTG

Cuts at:      0     145     939 
   Size:        145     794
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     939 
   Size:         75     864
 
ApoI r'AATT_y

Cuts at:      0     886     939 
   Size:        886      53
 
BanI G'GyrC_C

Cuts at:      0     118     939 
   Size:        118     821
 
BanII G_rGCy'C

Cuts at:      0     162     939 
   Size:        162     777
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     274     939 
   Size:        274     665
 
Bme1580I G_kGCm'C

Cuts at:      0     121     939 
   Size:        121     818
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     315     498     939 
   Size:         88     227     183     441

  Fragments arranged by size: 

                 441     227     183      88
 
BsiHKAI G_wGCw'C

Cuts at:      0     404     939 
   Size:        404     535
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     162     404     939 
   Size:        121      41     242     535

  Fragments arranged by size: 

                 535     242     121      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     260     939 
   Size:        260     679
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      75     939 
   Size:         75     864
 
BstEII G'GTnAC_C

Cuts at:      0     471     939 
   Size:        471     468
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     150     243     939 
   Size:        150      93     696

  Fragments arranged by size: 

                 696     150      93
 
BtgI C'CryG_G

Cuts at:      0     350     939 
   Size:        350     589
 
BtsI GCAGTG_nn'

Cuts at:      0     385     939 
   Size:        385     554
 
EaeI y'GGCC_r

Cuts at:      0      59     371     915     939 
   Size:         59     312     544      24

  Fragments arranged by size: 

                 544     312      59      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     319     939 
   Size:        319     620
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     564     939 
   Size:        564     375
 
EcoRI G'AATT_C

Cuts at:      0     886     939 
   Size:        886      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      20      52     241     273     855     887     939 
   Size:         20      32     189      32     582      32      52

  Fragments arranged by size: 

                 582     189      52      32      32      32      20
 
HincII GTy'rAC

Cuts at:      0     249     939 
   Size:        249     690
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     675     939 
   Size:        536     139     264

  Fragments arranged by size: 

                 536     264     139
 
MscI TGG'CCA

Cuts at:      0      61     373     939 
   Size:         61     312     566

  Fragments arranged by size: 

                 566     312      61
 
MslI CAynn'nnrTG

Cuts at:      0     145     298     598     939 
   Size:        145     153     300     341

  Fragments arranged by size: 

                 341     300     153     145
 
MspA1I CmG'CkG

Cuts at:      0      75     588     939 
   Size:         75     513     351

  Fragments arranged by size: 

                 513     351      75
 
NcoI C'CATG_G

Cuts at:      0     350     939 
   Size:        350     589
 
PstI C_TGCA'G

Cuts at:      0      71     939 
   Size:         71     868
 
PvuII CAG'CTG

Cuts at:      0      75     588     939 
   Size:         75     513     351

  Fragments arranged by size: 

                 513     351      75
 
SfcI C'TryA_G

Cuts at:      0      67     753     939 
   Size:         67     686     186

  Fragments arranged by size: 

                 686     186      67
 
SmlI C'TyrA_G

Cuts at:      0     777     939 
   Size:        777     162
 
StyI C'CwwG_G

Cuts at:      0     350     617     939 
   Size:        350     267     322

  Fragments arranged by size: 

                 350     322     267
 
TatI w'GTAC_w

Cuts at:      0     176     939 
   Size:        176     763
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     401     433     939 
   Size:        401      32     506

  Fragments arranged by size: 

                 506     401      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     147     279     939 
   Size:        147     132     660

  Fragments arranged by size: 

                 660     147     132
 

 Enzymes that do cut:

     AcuI    AflII     AhdI     AleI    AlwNI     ApoI     BanI    BanII
     BbsI Bme1580I    BseRI  BsiHKAI Bsp1286I    BspMI   BstAPI   BstEII
    BstXI     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoRI    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NcoI     PstI    PvuII
     SfcI     SmlI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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