Restriction map for pF1KE0856
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BcgI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MunI        MvnI        
NarI        NciI        NcoI        NdeI        NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        TaqI        Tru9I       
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI           31  GAATTT               [GA]^AATT[CT]
AlwI          522  GATCC                GGATC(4/5)
AlwNI          67  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           31  GAATTT               [GA]^AATT[CT]
AvaII         443  GGACC                G^G[AT]CC
BciVI         730  GTATCC               GTATCC(6/5)
BclI          363  TGATCA               T^GATCA
BglI          820  GCCTTGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         370  TACGTG               [CT]AC^GT[GA]
BsmFI         413  GTCCC                GGGAC(10/14)
BsmI          900  GAATGC               GAATGC(1/-1)
Bsp1407I      809  TGTACA               T^GTACA
BsrDI         116  CATTGC               GCAATG(2/0)
BsrFI         704  GCCGGC               [GA]^CCGG[CT]
BsrGI         809  TGTACA               T^GTACA
BstAPI         66  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        704  GCCGGC               [GA]^CCGG[CT]
DraII         794  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        367  CACTACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          417  CCGTGG               C^C[GA][CT]GG
EcoO109I      794  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       791  CCAAGG               C^C[AT][AT]GG
FbaI          363  TGATCA               T^GATCA
HapII         705  CCGG                 C^CGG
HpaII         705  CCGG                 C^CGG
MaeII         371  ACGT                 A^CGT
MspI          705  CCGG                 C^CGG
NaeI          704  GCCGGC               GCC^GGC
NgoMIV        704  GCCGGC               G^CCGGC
SapI          258  GCTCTTC              GCTCTTC(1/4)
SmlI          681  CTTGAG               C^T[CT][GA]AG
SphI          242  GCATGC               GCATG^C
SspBI         809  TGTACA               T^GTACA
StyI          791  CCAAGG               C^C[AT][AT]GG
Tsp509I        32  AATT                 ^AATT
XcmI          275  CCATCACCTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BalI          140  TGGCCA               TGG^CCA
              827  TGGCCA              
BbsI          661  GTCTTC               GAAGAC(2/6)
              844  GTCTTC              
BbvCI         858  CCTCAGC              CCTCAGC(-5/-2)
              697  GCTGAGG             
BbvI           66  GCAGC                GCAGC(8/12)
              673  GCTGC               
BmyI          194  GTGCCC               G[AGT]GC[ACT]^C
              425  GTGCCC              
BpuAI         661  GTCTTC               GAAGAC(2/6)
              844  GTCTTC              
Bsp1286I      194  GTGCCC               G[AGT]GC[ACT]^C
              425  GTGCCC              
BstF5I        648  CATCC                GGATG(2/0)
              470  GGATG               
EaeI          140  TGGCCA               [CT]^GGCC[GA]
              827  TGGCCA              
EarI          184  CTCTTC               CTCTTC(1/4)
              259  CTCTTC              
Fnu4HI         66  GCAGC                GC^[ACGT]GC
              673  GCTGC               
FokI          648  CATCC                GGATG(9/13)
              470  GGATG               
ItaI           66  GCAGC                GC^[ACGT]GC
              673  GCTGC               
Ksp632I       184  CTCTTC               CTCTTC(1/4)
              259  CTCTTC              
MluNI         140  TGGCCA               TGG^CCA
              827  TGGCCA              
MscI          140  TGGCCA               TGG^CCA
              827  TGGCCA              
NspI          242  GCATGC               [GA]CATG^[CT]
              401  ACATGC              
PflMI         143  CCACAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              429  CCATCTTGTGG         
PleI          164  GACTC                GAGTC(4/5)
              411  GAGTC               
SfaNI         603  GATGC                GCATC(5/9)
              817  GATGC               
SfcI           15  CTACAG               C^T[GA][CT]AG
              759  CTATAG              
TfiI          213  GATTC                G^A[AT]TC
              687  GATTC               
TseI           66  GCAGC                G^C[AT]GC
              673  GCTGC               
Van91I        143  CCACAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              429  CCATCTTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AfaI        AluI        BsaJI       BsiYI       BslI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      HaeIII      HinfI       HphI        
MaeIII      MboI        MboII       MnlI        MslI        
MvaI        MwoI        NdeII       NlaIII      RsaI        
Sau3AI      Sau96I      ScrFI       Tsp45I      TspRI       
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