Restriction map for pF1KE0856
 (Linear) (Six-base) MAPSORT of: KIEE0856.seq  Check: 9089  from: 1  to: 948



KIEE0856.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916     948 
   Size:        916      32
 
AleI CACnn'nnGTG

Cuts at:      0     283     745     948 
   Size:        283     462     203

  Fragments arranged by size: 

                 462     283     203
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     149     181     948 
   Size:        149      32     767

  Fragments arranged by size: 

                 767     149      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      72     948 
   Size:         72     876
 
ApoI r'AATT_y

Cuts at:      0      31     948 
   Size:         31     917
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     163     196     713     746     948 
   Size:        163      33     517      33     202

  Fragments arranged by size: 

                 517     202     163      33      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     654     837     948 
   Size:        654     183     111

  Fragments arranged by size: 

                 654     183     111
 
BbvCI CC'TCA_GC

Cuts at:      0     698     859     948 
   Size:        698     161      89

  Fragments arranged by size: 

                 698     161      89
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     741     948 
   Size:        741     207
 
BclI T'GATC_A

Cuts at:      0     363     948 
   Size:        363     585
 
BglI GCCn_nnn'nGGC

Cuts at:      0     826     948 
   Size:        826     122
 
Bme1580I G_kGCm'C

Cuts at:      0     198     429     948 
   Size:        198     231     519

  Fragments arranged by size: 

                 519     231     198
 
Bpu10I CC'TnA_GC

Cuts at:      0     698     859     948 
   Size:        698     161      89

  Fragments arranged by size: 

                 698     161      89
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     702     948 
   Size:        702     246
 
BsaAI yAC'GTr

Cuts at:      0     372     948 
   Size:        372     576
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     149     179     948 
   Size:        149      30     769

  Fragments arranged by size: 

                 769     149      30
 
BseYI C'CCAG_C

Cuts at:      0      62     948 
   Size:         62     886
 
BsmI GAATG_Cn'

Cuts at:      0     906     948 
   Size:        906      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     198     429     948 
   Size:        198     231     519

  Fragments arranged by size: 

                 519     231     198
 
BsrDI GCAATG_nn'

Cuts at:      0     115     948 
   Size:        115     833
 
BsrFI r'CCGG_y

Cuts at:      0     704     948 
   Size:        704     244
 
BsrGI T'GTAC_A

Cuts at:      0     809     948 
   Size:        809     139
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      72     948 
   Size:         72     876
 
BtgI C'CryG_G

Cuts at:      0     417     948 
   Size:        417     531
 
BtsI GCAGTG_nn'

Cuts at:      0     388     948 
   Size:        388     560
 
DraIII CAC_nnn'GTG

Cuts at:      0     372     948 
   Size:        372     576
 
EaeI y'GGCC_r

Cuts at:      0     140     827     948 
   Size:        140     687     121

  Fragments arranged by size: 

                 687     140     121
 
EarI CTCTTCn'nnn_

Cuts at:      0     190     265     948 
   Size:        190      75     683

  Fragments arranged by size: 

                 683     190      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916     948 
   Size:        916      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     795     948 
   Size:        795     153
 
MscI TGG'CCA

Cuts at:      0     142     829     948 
   Size:        142     687     119

  Fragments arranged by size: 

                 687     142     119
 
MslI CAynn'nnrTG

Cuts at:      0     175     283     301     352     406     469     598     745 
   Size:        175     108      18      51      54      63     129     147

Cuts at:    745     948 
   Size:        203

  Fragments arranged by size: 

                 203     175     147     129     108      63      54      51
                  18
 
NaeI GCC'GGC

Cuts at:      0     706     948 
   Size:        706     242
 
NgoMIV G'CCGG_C

Cuts at:      0     704     948 
   Size:        704     244
 
NspI r_CATG'y

Cuts at:      0     246     405     948 
   Size:        246     159     543

  Fragments arranged by size: 

                 543     246     159
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     149     435     948 
   Size:        149     286     513

  Fragments arranged by size: 

                 513     286     149
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     149     181     202     234     948 
   Size:        149      32      21      32     714

  Fragments arranged by size: 

                 714     149      32      32      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0     265     948 
   Size:        265     683
 
SfcI C'TryA_G

Cuts at:      0      15     759     948 
   Size:         15     744     189

  Fragments arranged by size: 

                 744     189      15
 
SmlI C'TyrA_G

Cuts at:      0     681     948 
   Size:        681     267
 
SphI G_CATG'C

Cuts at:      0     246     948 
   Size:        246     702
 
StyI C'CwwG_G

Cuts at:      0     791     948 
   Size:        791     157
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189     280     401     450     586     948 
   Size:        189      91     121      49     136     362

  Fragments arranged by size: 

                 362     189     136     121      91      49
 
TatI w'GTAC_w

Cuts at:      0     809     948 
   Size:        809     139
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     282     948 
   Size:        282     666
 

 Enzymes that do cut:

     AcuI     AleI     AloI    AlwNI     ApoI     BaeI     BbsI    BbvCI
    BciVI     BclI     BglI Bme1580I   Bpu10I    BpuEI    BsaAI    BsaXI
    BseYI     BsmI Bsp1286I    BsrDI    BsrFI    BsrGI   BstAPI     BtgI
     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I     MscI     MslI
     NaeI   NgoMIV     NspI    PflMI     PpiI     SapI     SfcI     SmlI
     SphI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BcgI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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