Restriction map for
pF1KE0855
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanII BbeI BbrPI BcgI BciVI
BcnI BfrI BglII BlnI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspHI
BspMI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cac8I ClaI CpoI DraI DraII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflIII 203 ACATGT A^C[GA][CT]GT
AocI 774 CCTGAGG CC^T[ACGT]AGG
AvaI 618 CTCGGG C^[CT]CG[GA]G
BamHI 677 GGATCC G^GATCC
BanI 225 GGTGCC G^G[CT][GA]CC
BbsI 82 GTCTTC GAAGAC(2/6)
BbvCI 572 CCTCAGC CCTCAGC(-5/-2)
BclI 137 TGATCA T^GATCA
BfaI 610 CTAG C^TAG
BglI 421 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 292 GCTCAGC GC^T[ACGT]AGC
BpmI 786 CTCCAG CTGGAG(16/14)
Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC
BpuAI 82 GTCTTC GAAGAC(2/6)
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaBI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 618 CTCGGG C^[CT]CG[GA]G
Bsp1407I 857 TGTACA T^GTACA
BspLU11I 203 ACATGT A^CATGT
BsrBI 382 CCGCTC CCGCTC(-3/-3)
BsrFI 433 ACCGGC [GA]^CCGG[CT]
BsrGI 857 TGTACA T^GTACA
BssSI 414 CTCGTG CACGAG(-5/-1)
BstXI 578 CCAACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 774 CCTGAGG CC^T[ACGT]AGG
CelII 292 GCTCAGC GC^T[ACGT]AGC
CfoI 411 GCGC GCG^C
Cfr10I 433 ACCGGC [GA]^CCGG[CT]
DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 243 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 774 CCTGAGG CC^T[ACGT]AGG
EcoT14I 245 CCTTGG C^C[AT][AT]GG
FauI 381 CCCGC CCCGC(4/6)
FbaI 137 TGATCA T^GATCA
HaeII 410 GGCGCT [GA]GCGC^[CT]
HapII 434 CCGG C^CGG
HhaI 411 GCGC GCG^C
HinP1I 411 GCGC G^CGC
HpaII 434 CCGG C^CGG
MaeI 610 CTAG C^TAG
MaeII 98 ACGT A^CGT
MamI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 83 TCTTC GAAGA(8/7)
MspA1I 282 CAGCTG C[AC]G^C[GT]G
MspI 434 CCGG C^CGG
NspI 203 ACATGT [GA]CATG^[CT]
PvuII 282 CAGCTG CAG^CTG
SspBI 857 TGTACA T^GTACA
SspI 601 AATATT AAT^ATT
StyI 245 CCTTGG C^C[AT][AT]GG
TfiI 148 GATTC G^A[AT]TC
XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 64 GACGCC G[GA]^CG[CT]C
793 GACGCC
AspHI 100 GTGCTC G[AT]GC[AT]^C
321 GAGCAC
AvaII 505 GGACC G^G[AT]CC
738 GGTCC
BalI 368 TGGCCA TGG^CCA
428 TGGCCA
BbvI 284 GCTGC GCAGC(8/12)
623 GCTGC
BsaHI 64 GACGCC G[GA]^CG[CT]C
793 GACGCC
BseRI 121 CTCCTC GAGGAG(10/8)
836 CTCCTC
BsgI 457 CTGCAC GTGCAG(16/14)
563 GTGCAG
BsiHKAI 100 GTGCTC G[AT]GC[AT]^C
321 GAGCAC
BsmFI 791 GGGAC GGGAC(10/14)
252 GTCCC
BsrI 406 ACTGG ACTGG(1/-1)
555 ACTGG
BstYI 515 AGATCC [GA]^GATC[CT]
677 GGATCC
Hin1I 64 GACGCC G[GA]^CG[CT]C
793 GACGCC
MflI 515 AGATCC [GA]^GATC[CT]
677 GGATCC
MluNI 368 TGGCCA TGG^CCA
428 TGGCCA
MscI 368 TGGCCA TGG^CCA
428 TGGCCA
PflMI 70 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
309 CCACTTCCTGG
PleI 834 GACTC GAGTC(4/5)
80 GAGTC
TseI 284 GCTGC G^C[AT]GC
623 GCTGC
Tsp45I 220 GTCAC ^GT[GC]AC
533 GTGAC
Van91I 70 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
309 CCACTTCCTGG
XhoII 515 AGATCC [GA]^GATC[CT]
677 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AlwI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI MaeIII
MboI MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TspRI