Restriction map for pF1KE0853
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       BspDI       
BspEI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DpnII       
DraI        DraII       EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HinfI       HpaI        KspI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        NcoI        NdeI        NdeII       
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII         619  GACGTC               GACGT^C
Acc65I        380  GGTACC               G^GTACC
AccI          763  GTCTAC               GT^[AC][GT]AC
AccII         559  CGCG                 CG^CG
AflIII        224  ACATGT               A^C[GA][CT]GT
AocI          792  CCTTAGG              CC^T[ACGT]AGG
ApaI          523  GGGCCC               GGGCC^C
Asp718I       380  GGTACC               G^GTACC
BanII         523  GGGCCC               G[GA]GC[CT]^C
BbeI          716  GGCGCC               GGCGC^C
BbsI          100  GTCTTC               GAAGAC(2/6)
BcnI          720  CCGGG                CC^[GC]GG
BfaI          385  CTAG                 C^TAG
BpmI          452  CTGGAG               CTGGAG(16/14)
BpuAI         100  GTCTTC               GAAGAC(2/6)
BsgI          403  CTGCAC               GTGCAG(16/14)
BsmFI         772  GTCCC                GGGAC(10/14)
Bsp1407I      191  TGTACA               T^GTACA
BspHI         419  TCATGA               T^CATGA
BspLU11I      224  ACATGT               A^CATGT
BsrFI         466  GCCGGC               [GA]^CCGG[CT]
BsrGI         191  TGTACA               T^GTACA
BstAPI        341  GCACCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         559  CGCG                 CG^CG
BstXI         527  CCAATCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        792  CCTTAGG              CC^T[ACGT]AGG
Cfr10I        466  GCCGGC               [GA]^CCGG[CT]
DrdI          586  GACACAACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           32  CCGTGG               C^C[GA][CT]GG
EaeI          632  TGGCCG               [CT]^GGCC[GA]
Eco81I        792  CCTTAGG              CC^T[ACGT]AGG
EcoNI         371  CCTATGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       475  ATGCAT               ATGCA^T
HaeII         716  GGCGCC               [GA]GCGC^[CT]
KasI          716  GGCGCC               G^GCGCC
KpnI          380  GGTACC               GGTAC^C
MaeI          385  CTAG                 C^TAG
MaeII         620  ACGT                 A^CGT
MseI          652  TTAA                 T^TAA
MslI          360  CACCTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          559  CGCG                 CG^CG
NaeI          466  GCCGGC               GCC^GGC
NarI          716  GGCGCC               GG^CGCC
NciI          720  CCGGG                CC^[GC]GG
NgoMIV        466  GCCGGC               G^CCGGC
NsiI          475  ATGCAT               ATGCA^T
NspI          224  ACATGT               [GA]CATG^[CT]
PvuII         127  CAGCTG               CAG^CTG
RcaI          419  TCATGA               T^CATGA
SfaNI          28  GATGC                GCATC(5/9)
SfoI          716  GGCGCC               GGC^GCC
SspBI         191  TGTACA               T^GTACA
Tru9I         652  TTAA                 T^TAA
Tsp509I       654  AATT                 ^AATT
XcmI          806  CCAAAGACCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          619  GACGTC               G[GA]^CG[CT]C
              716  GGCGCC              
AfaI          192  GTAC                 GT^AC
              381  GTAC                
AlwNI         397  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              827  CAGCGGCTG           
BanI          380  GGTACC               G^G[CT][GA]CC
              716  GGCGCC              
BbvI          338  GCAGC                GCAGC(8/12)
              826  GCAGC               
BsaHI         619  GACGTC               G[GA]^CG[CT]C
              716  GGCGCC              
BseRI          88  CTCCTC               GAGGAG(10/8)
              234  CTCCTC              
BsmAI         570  GAGAC                GTCTC(1/5)
               63  GTCTC               
BspMI         137  ACCTGC               ACCTGC(4/8)
              925  GCAGGT              
CfoI          560  GCGC                 GCG^C
              717  GCGC                
DraIII        153  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
              342  CACCCAGTG           
EarI           91  CTCTTC               CTCTTC(1/4)
              912  GAAGAG              
HapII         467  CCGG                 C^CGG
              720  CCGG                
HhaI          560  GCGC                 GCG^C
              717  GCGC                
Hin1I         619  GACGTC               G[GA]^CG[CT]C
              716  GGCGCC              
HinP1I        560  GCGC                 G^CGC
              717  GCGC                
HpaII         467  CCGG                 C^CGG
              720  CCGG                
Ksp632I        91  CTCTTC               CTCTTC(1/4)
              912  GAAGAG              
MspI          467  CCGG                 C^CGG
              720  CCGG                
RsaI          192  GTAC                 GT^AC
              381  GTAC                
TseI          338  GCAGC                G^C[AT]GC
              826  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        AspHI       BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HphI        ItaI        MaeIII      MboII       MnlI        
MspA1I      MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com