Restriction map for pF1KE0853
 (Linear) (Six-base) MAPSORT of: KIEE0853.seq  Check: 5779  from: 1  to: 930



KIEE0853.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AatII G_ACGT'C

Cuts at:      0     623     930 
   Size:        623     307
 
AccI GT'mk_AC

Cuts at:      0     764     930 
   Size:        764     166
 
Acc65I G'GTAC_C

Cuts at:      0     380     930 
   Size:        380     550
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712     930 
   Size:        712     218
 
AflIII A'CryG_T

Cuts at:      0     224     930 
   Size:        224     706
 
AlwNI CAG_nnn'CTG

Cuts at:      0     402     832     930 
   Size:        402     430      98

  Fragments arranged by size: 

                 430     402      98
 
ApaI G_GGCC'C

Cuts at:      0     527     930 
   Size:        527     403
 
BanI G'GyrC_C

Cuts at:      0     380     716     930 
   Size:        380     336     214

  Fragments arranged by size: 

                 380     336     214
 
BanII G_rGCy'C

Cuts at:      0     527     930 
   Size:        527     403
 
BbeI G_GCGC'C

Cuts at:      0     720     930 
   Size:        720     210
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      93     930 
   Size:         93     837
 
BfrBI ATG'CAT

Cuts at:      0     477     930 
   Size:        477     453
 
Bme1580I G_kGCm'C

Cuts at:      0     527     930 
   Size:        527     403
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     339     406     930 
   Size:        339      67     524

  Fragments arranged by size: 

                 524     339      67
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     930 
   Size:        473     457
 
BsaHI Gr'CG_yC

Cuts at:      0     620     717     930 
   Size:        620      97     213

  Fragments arranged by size: 

                 620     213      97
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     446     476     930 
   Size:        446      30     454

  Fragments arranged by size: 

                 454     446      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     225     930 
   Size:         79     146     705

  Fragments arranged by size: 

                 705     146      79
 
BseYI C'CCAG_C

Cuts at:      0     129     930 
   Size:        129     801
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     930 
   Size:        388     542
 
BsiHKAI G_wGCw'C

Cuts at:      0     183     212     419     930 
   Size:        183      29     207     511

  Fragments arranged by size: 

                 511     207     183      29
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     212     419     527     930 
   Size:        183      29     207     108     403

  Fragments arranged by size: 

                 403     207     183     108      29
 
BspHI T'CATG_A

Cuts at:      0     419     930 
   Size:        419     511
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146     916     930 
   Size:        146     770      14

  Fragments arranged by size: 

                 770     146      14
 
BsrFI r'CCGG_y

Cuts at:      0     466     930 
   Size:        466     464
 
BsrGI T'GTAC_A

Cuts at:      0     191     930 
   Size:        191     739
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     347     930 
   Size:        347     583
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     534     930 
   Size:        534     396
 
Bsu36I CC'TnA_GG

Cuts at:      0     793     930 
   Size:        793     137
 
BtgI C'CryG_G

Cuts at:      0      32     930 
   Size:         32     898
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       5     930 
   Size:          5     925
 
DraIII CAC_nnn'GTG

Cuts at:      0     158     347     930 
   Size:        158     189     583

  Fragments arranged by size: 

                 583     189     158
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     592     930 
   Size:        592     338
 
EaeI y'GGCC_r

Cuts at:      0     632     930 
   Size:        632     298
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     907     930 
   Size:         97     810      23

  Fragments arranged by size: 

                 810      97      23
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     496     930 
   Size:        496     434
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     712     930 
   Size:        473     239     218

  Fragments arranged by size: 

                 473     239     218
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     375     930 
   Size:        375     555
 
HaeII r_GCGC'y

Cuts at:      0     720     930 
   Size:        720     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     446     478     930 
   Size:        446      32     452

  Fragments arranged by size: 

                 452     446      32
 
KasI G'GCGC_C

Cuts at:      0     716     930 
   Size:        716     214
 
KpnI G_GTAC'C

Cuts at:      0     384     930 
   Size:        384     546
 
MslI CAynn'nnrTG

Cuts at:      0     364     930 
   Size:        364     566
 
MspA1I CmG'CkG

Cuts at:      0     129     706     829     930 
   Size:        129     577     123     101

  Fragments arranged by size: 

                 577     129     123     101
 
NaeI GCC'GGC

Cuts at:      0     468     930 
   Size:        468     462
 
NarI GG'CG_CC

Cuts at:      0     717     930 
   Size:        717     213
 
NgoMIV G'CCGG_C

Cuts at:      0     466     930 
   Size:        466     464
 
NsiI A_TGCA'T

Cuts at:      0     479     930 
   Size:        479     451
 
NspI r_CATG'y

Cuts at:      0     228     930 
   Size:        228     702
 
PciI A'CATG_T

Cuts at:      0     224     930 
   Size:        224     706
 
PfoI T'CCnGG_A

Cuts at:      0     218     930 
   Size:        218     712
 
PspOMI G'GGCC_C

Cuts at:      0     523     930 
   Size:        523     407
 
PvuII CAG'CTG

Cuts at:      0     129     930 
   Size:        129     801
 
SfoI GGC'GCC

Cuts at:      0     718     930 
   Size:        718     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     358     930 
   Size:        358     572
 
TatI w'GTAC_w

Cuts at:      0     191     930 
   Size:        191     739
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     813     930 
   Size:        813     117
 
ZraI GAC'GTC

Cuts at:      0     621     930 
   Size:        621     309
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII    AlwNI     ApaI     BanI
    BanII     BbeI     BbsI    BfrBI Bme1580I     BmrI     BpmI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrFI    BsrGI   BstAPI    BstXI   Bsu36I     BtgI    BtgZI   DraIII
     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI    HaeII    Hin4I
     KasI     KpnI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI
     NspI     PciI     PfoI   PspOMI    PvuII     SfoI    TaqII     TatI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I     BtsI
     ClaI    CspCI     DraI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI
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