Restriction map for pF1KE0852
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstYI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MroI        MseI        MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI           25  GAATTC               [GA]^AATT[CT]
AfaI          320  GTAC                 GT^AC
AlwI          465  GATCC                GGATC(4/5)
AocI          861  CCTTAGG              CC^T[ACGT]AGG
Aor51HI       718  AGCGCT               AGC^GCT
ApoI           25  GAATTC               [GA]^AATT[CT]
AvaII         434  GGACC                G^G[AT]CC
AvrII         312  CCTAGG               C^CTAGG
BalI          425  TGGCCA               TGG^CCA
BlnI          312  CCTAGG               C^CTAGG
BlpI          680  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      680  GCTCAGC              GC^T[ACGT]AGC
BspHI         398  TCATGA               T^CATGA
BspMI         712  ACCTGC               ACCTGC(4/8)
BsrBI         678  CCGCTC               CCGCTC(-3/-3)
BstEII        828  GGTAACC              G^GT[ACGT]ACC
BstPI         828  GGTAACC              G^GT[ACGT]ACC
Bsu36I        861  CCTTAGG              CC^T[ACGT]AGG
CelII         680  GCTCAGC              GC^T[ACGT]AGC
CfoI          719  GCGC                 GCG^C
DraIII        406  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          428  CCACGG               C^C[GA][CT]GG
EaeI          425  TGGCCA               [CT]^GGCC[GA]
EarI          213  CTCTTC               CTCTTC(1/4)
Eco47III      718  AGCGCT               AGC^GCT
Eco81I        861  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       828  GGTAACC              G^GT[ACGT]ACC
EcoRI          25  GAATTC               G^AATTC
EcoT14I       312  CCTAGG               C^C[AT][AT]GG
FauI          699  GCGGG                CCCGC(4/6)
HaeII         718  AGCGCT               [GA]GCGC^[CT]
HhaI          719  GCGC                 GCG^C
HinP1I        719  GCGC                 G^CGC
Ksp632I       213  CTCTTC               CTCTTC(1/4)
MaeII         119  ACGT                 A^CGT
MluNI         425  TGGCCA               TGG^CCA
MscI          425  TGGCCA               TGG^CCA
MspA1I        697  CAGCGG               C[AC]G^C[GT]G
NdeI          369  CATATG               CA^TATG
PpuMI         433  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          398  TCATGA               T^CATGA
RsaI          320  GTAC                 GT^AC
StyI          312  CCTAGG               C^C[AT][AT]GG
TaqI          153  TCGA                 T^CGA
TthHB8I       153  TCGA                 T^CGA
XcmI          194  CCATCTTTGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AluI          135  AGCT                 AG^CT
              293  AGCT                
BseRI          87  CTCCTC               GAGGAG(10/8)
              144  CTCCTC              
BsiYI         493  CCTTACTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              504  CCCTAACAAGG         
BslI          493  CCTTACTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              504  CCCTAACAAGG         
BsmFI         433  GGGAC                GGGAC(10/14)
              288  GTCCC               
BssKI         204  CCTGG                ^CC[ACGT]GG
              437  CCTGG               
BstNI         204  CCTGG                CC^[AT]GG
              437  CCTGG               
DpnII         465  GATC                 ^GATC
              886  GATC                
DraII          56  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              433  GGGACCT             
EcoO109I       56  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              433  GGGACCT             
EcoRII        204  CCTGG                ^CC[AT]GG
              437  CCTGG               
HinfI          41  GAATC                G^A[ACGT]TC
              843  GAATC               
MboI          465  GATC                 ^GATC
              886  GATC                
MboII         214  TCTTC                GAAGA(8/7)
              299  TCTTC               
MvaI          204  CCTGG                CC^[AT]GG
              437  CCTGG               
NdeII         465  GATC                 ^GATC
              886  GATC                
NlaIV         433  GGGACC               GG[ACGT]^[ACGT]CC
              799  GGTTCC              
PstI          539  CTGCAG               CTGCA^G
              714  CTGCAG              
Sau3AI        465  GATC                 ^GATC
              886  GATC                
ScrFI         204  CCTGG                CC^[ACGT]GG
              437  CCTGG               
TfiI           41  GAATC                G^A[AT]TC
              843  GAATC               
Tsp45I         19  GTGAC                ^GT[GC]AC
              353  GTGAC               
Tsp509I        26  AATT                 ^AATT
              807  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        BbvI        BfaI        BsaJI       BsmAI       
BsrI        BstF5I      Cac8I       Cfr13I      DdeI        
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MaeI        MaeIII      MnlI        MslI        NlaIII      
Sau96I      SfcI        TseI        TspRI       
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